{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:35:01Z","timestamp":1787841301905,"version":"build-2784847793"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,6,18]],"date-time":"2016-06-18T00:00:00Z","timestamp":1466208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective\/background:<\/jats:title>\n                    <jats:p>\n                      Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t(8;14)(q24;q32), which involves the\n                      <jats:italic toggle=\"yes\">c-myc<\/jats:italic>\n                      gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene\/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14)\n                      <jats:italic toggle=\"yes\">c-myc\/IgH<\/jats:italic>\n                      gene rearrangement by LD-PCR in patients with DLBCL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the\n                      <jats:italic toggle=\"yes\">IgH<\/jats:italic>\n                      gene (joining region and \u03b3 switch region) and the\n                      <jats:italic toggle=\"yes\">myc<\/jats:italic>\n                      gene (Exons 2 and 3).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      As much as 1.85% of the samples were positive for the \u03b3 constant region and Exon 2 of the\n                      <jats:italic toggle=\"yes\">myc<\/jats:italic>\n                      gene.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      LD-PCR can be used for the detection of t(8;14)\n                      <jats:italic toggle=\"yes\">c-myc\/IgH<\/jats:italic>\n                      gene rearrangement in patients with DLBCL.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1016\/j.hemonc.2016.05.006","type":"journal-article","created":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T09:52:08Z","timestamp":1466675528000},"page":"141-146","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":14,"title":["Detection of t(8;14) c-myc\/IgH gene rearrangement by long-distance polymerase chain reaction in patients with diffuse large B-cell 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