{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:40:35Z","timestamp":1770597635062,"version":"3.49.0"},"reference-count":33,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Human Immunology"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1016\/j.humimm.2019.03.015","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T14:32:33Z","timestamp":1553092353000},"page":"487-492","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Patients with immunological diseases or on peritoneal dialysis are prone to false positive flow cytometry crossmatch"],"prefix":"10.1016","volume":"80","author":[{"given":"Qingyong","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew A.","family":"House","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Leckie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8845-1126","authenticated-orcid":false,"given":"Lakshman","family":"Gunaratnam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick P.","family":"Luke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony M.","family":"Jevnikar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"14","key":"10.1016\/j.humimm.2019.03.015_b0005","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1056\/NEJM196904032801401","article-title":"Significance of the positive crossmatch test in kidney transplantation","volume":"280","author":"Patel","year":"1969","journal-title":"N. Engl. J. Med."},{"issue":"12","key":"10.1016\/j.humimm.2019.03.015_b0010","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1046\/j.1600-6135.2003.00273.x","article-title":"Pre-transplant assessment of donor-reactive, HLA-specific antibodies in renal transplantation: contraindication vs. risk","volume":"3","author":"Gebel","year":"2003","journal-title":"Am. J. Transplant."},{"key":"10.1016\/j.humimm.2019.03.015_b0015","doi-asserted-by":"crossref","DOI":"10.1111\/ajt.14752","article-title":"Sensitization in transplantation: assessment of risk (STAR) 2017 working group meeting report","author":"Tambur","year":"2018","journal-title":"Am. J. Transplant."},{"issue":"12","key":"10.1016\/j.humimm.2019.03.015_b0020","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.1681\/ASN.V12122807","article-title":"Flow cytometric crossmatching in primary renal transplant recipients with a negative anti-human globulin enhanced cytotoxicity crossmatch","volume":"12","author":"Karpinski","year":"2001","journal-title":"J. Am. Soc. Nephrol."},{"issue":"5","key":"10.1016\/j.humimm.2019.03.015_b0025","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1097\/00007890-198911000-00022","article-title":"The flow cytometric crossmatch. Dual-color analysis of T cell and B cell reactivities","volume":"48","author":"Bray","year":"1989","journal-title":"Transplantation"},{"issue":"3","key":"10.1016\/j.humimm.2019.03.015_b0030","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1097\/00007890-200102150-00015","article-title":"Improved flow cytometric detection of HLA alloantibodies using pronase: potential implications in renal transplantation","volume":"71","author":"Vaidya","year":"2001","journal-title":"Transplantation"},{"issue":"1S Suppl 1","key":"10.1016\/j.humimm.2019.03.015_b0035","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1097\/TP.0000000000001818","article-title":"Of cells and microparticles: assets and liabilities of HLA antibody detection","volume":"102","author":"Liwski","year":"2018","journal-title":"Transplantation"},{"issue":"8","key":"10.1016\/j.humimm.2019.03.015_b0040","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1111\/j.1600-6143.2009.02724.x","article-title":"Perception versus reality?: Virtual crossmatch\u2013how to overcome some of the technical and logistic limitations","volume":"9","author":"Tambur","year":"2009","journal-title":"Am. J. Transplant."},{"issue":"2","key":"10.1016\/j.humimm.2019.03.015_b0045","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.transproceed.2004.12.066","article-title":"New crossmatch technique eliminates interference by humanized and chimeric monoclonal antibodies","volume":"37","author":"Book","year":"2005","journal-title":"Transplant. Proc."},{"issue":"3","key":"10.1016\/j.humimm.2019.03.015_b0050","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.trim.2015.04.004","article-title":"Interference of therapeutic antibodies used in desensitization protocols on lymphocytotoxicity crossmatch results","volume":"32","author":"Milongo","year":"2015","journal-title":"Transpl. Immunol."},{"issue":"5","key":"10.1016\/j.humimm.2019.03.015_b0055","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1097\/TP.0b013e31822872dd","article-title":"HLA antibody specification using single-antigen beads\u2013a technical solution for the prozone effect","volume":"92","author":"Schnaidt","year":"2011","journal-title":"Transplantation"},{"issue":"7\u20138","key":"10.1016\/j.humimm.2019.03.015_b0060","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.humimm.2017.05.001","article-title":"It's about time: the development and validation of a rapid optimized single antigen bead (ROB) assay protocol for LABScreen","volume":"78","author":"Liwski","year":"2017","journal-title":"Hum. Immunol."},{"issue":"1","key":"10.1016\/j.humimm.2019.03.015_b0065","doi-asserted-by":"crossref","first-page":"37","DOI":"10.21037\/hbsn.2019.01.01","article-title":"Detecting DSA: the importance of sorting the wheat from the chaff","volume":"8","author":"McCaughan","year":"2019","journal-title":"Hepatobiliary Surg. Nutr."},{"issue":"12","key":"10.1016\/j.humimm.2019.03.015_b0070","doi-asserted-by":"crossref","first-page":"3194","DOI":"10.1111\/ajt.13355","article-title":"cPRA increases with DQA, DPA, and DPB unacceptable antigens in the Canadian cPRA calculator","volume":"15","author":"Tinckam","year":"2015","journal-title":"Am. J. Transplant."},{"key":"10.1016\/j.humimm.2019.03.015_b0075","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.humimm.2012.07.062","article-title":"30-OR: Canada-wide evaluation of rapid optimized flow crossmatch (ROFCXM) protocol","volume":"73","author":"Liwski","year":"2012","journal-title":"Hum. Immunol."},{"issue":"4","key":"10.1016\/j.humimm.2019.03.015_b0080","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1097\/MOT.0000000000000423","article-title":"Becoming a chef in the human leukocyte antigen kitchen: interpretation and modification of human leukocyte antigen-antibody assays","volume":"22","author":"Liwski","year":"2017","journal-title":"Curr. Opin. Organ Transplant."},{"key":"10.1016\/j.humimm.2019.03.015_b0085","doi-asserted-by":"crossref","DOI":"10.1681\/ASN.2011050464","article-title":"The pathophysiology of IgA nephropathy","author":"Suzuki","year":"2011","journal-title":"J. Am. Soc. Nephrol."},{"key":"10.1016\/j.humimm.2019.03.015_b0090","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1159\/000382134","article-title":"New insights into the pathogenesis of IgA nephropathy","author":"Novak","year":"2015","journal-title":"Kidney Dis. (Basel)"},{"issue":"11","key":"10.1016\/j.humimm.2019.03.015_b0095","doi-asserted-by":"crossref","first-page":"4053","DOI":"10.1093\/ndt\/gfs130","article-title":"Acidic stress-ER stress axis for blunted activation of NF-kappaB in mesothelial cells exposed to peritoneal dialysis fluid","volume":"27","author":"Johno","year":"2012","journal-title":"Nephrol. Dial. Transplant."},{"issue":"4","key":"10.1016\/j.humimm.2019.03.015_b0100","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1189\/jlb.0902451","article-title":"Peritoneal dialysis solutions inhibit the differentiation and maturation of human monocyte-derived dendritic cells: effect of lactate and glucose-degradation products","volume":"73","author":"Puig-Kroger","year":"2003","journal-title":"J. Leukoc. Biol."},{"issue":"2","key":"10.1016\/j.humimm.2019.03.015_b0105","first-page":"219","article-title":"The frequency of Th2 type cells increases with time on peritoneal dialysis in patients with diabetic nephropathy","volume":"10","author":"Zamauskaite","year":"1999","journal-title":"Eur. Cytokine Netw."},{"issue":"1","key":"10.1016\/j.humimm.2019.03.015_b0110","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1684\/ecn.2011.0280","article-title":"Effects of different peritoneal dialysis fluids on the TH1\/TH2 balance","volume":"22","author":"Libetta","year":"2011","journal-title":"Eur. Cytokine Netw."},{"issue":"8","key":"10.1016\/j.humimm.2019.03.015_b0115","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1093\/ndt\/15.8.1217","article-title":"T-cell activation follows Th1 rather than Th2 pattern in haemodialysis patients","volume":"15","author":"Sester","year":"2000","journal-title":"Nephrol. Dial. Transplant."},{"issue":"6","key":"10.1016\/j.humimm.2019.03.015_b0120","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1159\/000141041","article-title":"Receptor-interacting protein-2 deficiency delays macrophage migration and increases intracellular infection during peritoneal dialysis-associated peritonitis","volume":"28","author":"McCully","year":"2008","journal-title":"Am. J. Nephrol."},{"issue":"6","key":"10.1016\/j.humimm.2019.03.015_b0125","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.humimm.2016.04.014","article-title":"Flow cytometry crossmatch reactivity with pronase-treated T cells induced by non-HLA autoantibodies in human immunodeficiency virus-infected patients","volume":"77","author":"Szewczyk","year":"2016","journal-title":"Hum. Immunol."},{"issue":"11\u201312","key":"10.1016\/j.humimm.2019.03.015_b0130","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.humimm.2017.10.002","article-title":"Reanalysis of the role of pronase treatment of B cells in the flow cytometric crossmatch assay: Fc receptor is not the primary target","volume":"78","author":"Brown","year":"2017","journal-title":"Hum. Immunol."},{"issue":"10","key":"10.1016\/j.humimm.2019.03.015_b0135","doi-asserted-by":"crossref","first-page":"4191","DOI":"10.1016\/j.transproceed.2005.10.051","article-title":"Elevated B-cell autoantibodies among HIV+ transplant candidates: crossmatch implications","volume":"37","author":"Kimball","year":"2005","journal-title":"Transplant Proc."},{"issue":"6","key":"10.1016\/j.humimm.2019.03.015_b0140","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1111\/j.1432-2277.1995.tb01558.x","article-title":"The use of pronase-digested human leukocytes to improve specificity of the flow cytometric crossmatch","volume":"8","author":"Lobo","year":"1995","journal-title":"Transpl. Int."},{"issue":"10S","key":"10.1016\/j.humimm.2019.03.015_b0145","doi-asserted-by":"crossref","DOI":"10.1097\/00007890-201211271-01557","article-title":"Pronase treatment interfering with T cell crossmatch: 2403: transplantation","volume":"94","author":"Backer","year":"2012","journal-title":"Transplantation"},{"issue":"1","key":"10.1016\/j.humimm.2019.03.015_b0150","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.transproceed.2011.12.048","article-title":"Frequent false-positive reactions in pronase-treated T-cell flow cytometric cross-match tests","volume":"44","author":"Park","year":"2012","journal-title":"Transplant Proc."},{"issue":"3","key":"10.1016\/j.humimm.2019.03.015_b0155","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.transproceed.2014.12.022","article-title":"Falsely incompatible B-cell flow cytometry crossmatch after pronase treatment: a case report","volume":"47","author":"Hart","year":"2015","journal-title":"Transplant Proc."},{"issue":"4","key":"10.1016\/j.humimm.2019.03.015_b0160","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.humimm.2011.01.005","article-title":"Impact of pronase on flow cytometric crossmatch outcome","volume":"72","author":"Hetrick","year":"2011","journal-title":"Hum. Immunol."},{"key":"10.1016\/j.humimm.2019.03.015_b0165","article-title":"A novel DSA flow cytometer crossmatch (DSA-FXM) to eliminate ambiguous auto- and allo-FXM results (abstracts)","volume":"15","author":"Chen","year":"2015","journal-title":"Am. J. Transplant."}],"container-title":["Human Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0198885918310991?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0198885918310991?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T01:58:18Z","timestamp":1663120698000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0198885918310991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["S0198885918310991"],"URL":"https:\/\/doi.org\/10.1016\/j.humimm.2019.03.015","relation":{},"ISSN":["0198-8859"],"issn-type":[{"value":"0198-8859","type":"print"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Patients with immunological diseases or on peritoneal dialysis are prone to false positive flow cytometry crossmatch","name":"articletitle","label":"Article Title"},{"value":"Human Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.humimm.2019.03.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}