{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T18:25:46Z","timestamp":1759602346874},"reference-count":31,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2014,10,11]],"date-time":"2014-10-11T00:00:00Z","timestamp":1412985600000},"content-version":"vor","delay-in-days":344,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Human Pathology"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1016\/j.humpath.2013.04.026","type":"journal-article","created":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T22:04:58Z","timestamp":1376949898000},"page":"2410-2418","source":"Crossref","is-referenced-by-count":9,"title":["Human papillomavirus multiplex ligation-dependent probe amplification assay for the assessment of viral load, integration, and gain of telomerase-related genes in cervical malignancies"],"prefix":"10.1016","volume":"44","author":[{"given":"Wendy","family":"Theelen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rogier J.N.T.M.","family":"Litjens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Vinokurova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annick","family":"Haesevoets","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Reijans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guus","family":"Simons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Smedts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Simon","family":"Herrington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frans C.S.","family":"Ramaekers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"von Knebel Doeberitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernst-Jan M.","family":"Speel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton H.N.","family":"Hopman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.humpath.2013.04.026_bb0005","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3322\/caac.20107","article-title":"Global cancer statistics","volume":"61","author":"Jemal","year":"2011","journal-title":"CA Cancer J Clin"},{"key":"10.1016\/j.humpath.2013.04.026_bb0010","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1111\/j.1525-1438.2005.00246.x","article-title":"Human papillomavirus infection: biology, epidemiology, and prevention","volume":"15","author":"Scheurer","year":"2005","journal-title":"Int J Gynecol Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0015","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F","article-title":"Human papillomavirus is a necessary cause of invasive cervical cancer worldwide","volume":"189","author":"Walboomers","year":"1999","journal-title":"J Pathol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0020","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1002\/1097-0142(20011201)92:11<2875::AID-CNCR10130>3.0.CO;2-7","article-title":"Prevalence, distribution, and viral load of human papillomavirus 16 DNA in tonsillar carcinomas","volume":"92","author":"Klussmann","year":"2001","journal-title":"Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0025","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1097\/01.qai.0000179460.61987.33","article-title":"High-resolution analysis of genomic alterations and human papillomavirus integration in anal intraepithelial neoplasia","volume":"40","author":"Gagne","year":"2005","journal-title":"J Acquir Immune Defic Syndr"},{"key":"10.1016\/j.humpath.2013.04.026_bb0030","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1002\/ijc.23707","article-title":"Human papillomavirus-16 and -18 in penile carcinomas: DNA methylation, chromosomal recombination and genomic variation","volume":"123","author":"Kalantari","year":"2008","journal-title":"Int J Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0035","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1136\/jcp.55.4.244","article-title":"The causal relation between human papillomavirus and cervical cancer","volume":"55","author":"Bosch","year":"2002","journal-title":"J Clin Pathol"},{"issue":"Suppl 3","key":"10.1016\/j.humpath.2013.04.026_bb0040","doi-asserted-by":"crossref","first-page":"S26","DOI":"10.1016\/j.vaccine.2006.05.026","article-title":"Chapter 3: HPV type-distribution in women with and without cervical neoplastic diseases","volume":"24","author":"Clifford","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.humpath.2013.04.026_bb0045","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1155\/2007\/678793","article-title":"Biomarkers in cervical cancer screening","volume":"23","author":"Wentzensen","year":"2007","journal-title":"Dis Markers"},{"key":"10.1016\/j.humpath.2013.04.026_bb0050","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1128\/JVI.65.2.606-612.1991","article-title":"Analysis of the physical state of different human papillomavirus DNAs in intraepithelial and invasive cervical neoplasm","volume":"65","author":"Cullen","year":"1991","journal-title":"J Virol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0055","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/jmv.1890440216","article-title":"Analysis of deletion of the integrated human papillomavirus 16 sequence in cervical cancer: a rapid multiplex polymerase chain reaction approach","volume":"44","author":"Chen","year":"1994","journal-title":"J Med Virol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0060","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1128\/JCM.44.5.1755-1762.2006","article-title":"Human papillomavirus type 16 integration in cervical carcinoma in situ and in invasive cervical cancer","volume":"44","author":"Arias-Pulido","year":"2006","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0065","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/(SICI)1097-0215(19970220)74:1<50::AID-IJC9>3.0.CO;2-#","article-title":"Association of human papillomavirus type 16 integration in the E2 gene with poor disease-free survival from cervical cancer","volume":"74","author":"Vernon","year":"1997","journal-title":"Int J Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0070","first-page":"6132","article-title":"Detection of high-risk cervical intraepithelial neoplasia and cervical cancer by amplification of transcripts derived from integrated papillomavirus oncogenes","volume":"59","author":"Klaes","year":"1999","journal-title":"Cancer Res"},{"key":"10.1016\/j.humpath.2013.04.026_bb0075","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.5858\/132.12.1929","article-title":"Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue","volume":"132","author":"Hewitt","year":"2008","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.humpath.2013.04.026_bb0080","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/1097-0215(200102)9999:9999<::AID-IJC1144>3.0.CO;2-L","article-title":"Detection of integrated papillomavirus sequences by ligation-mediated PCR (DIPS-PCR) and molecular characterization in cervical cancer cells","volume":"92","author":"Luft","year":"2001","journal-title":"Int J Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0085","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1086\/509622","article-title":"Viral load, E2 gene disruption status, and lineage of human papillomavirus type 16 infection in cervical neoplasia","volume":"194","author":"Cheung","year":"2006","journal-title":"J Infect Dis"},{"key":"10.1016\/j.humpath.2013.04.026_bb0090","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1128\/JCM.40.3.886-891.2002","article-title":"Integrated human papillomavirus type 16 is frequently found in cervical cancer precursors as demonstrated by a novel quantitative real-time PCR technique","volume":"40","author":"Peitsaro","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0095","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1002\/ijc.24844","article-title":"A new multiparameter assay to assess HPV 16\/18, viral load and physical status together with gain of telomerase genes in HPV-related cancers","volume":"126","author":"Theelen","year":"2010","journal-title":"Int J Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0100","doi-asserted-by":"crossref","first-page":"e57","DOI":"10.1093\/nar\/gnf056","article-title":"Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification","volume":"30","author":"Schouten","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.humpath.2013.04.026_bb0105","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S1673-8527(08)60113-7","article-title":"Designing a simple multiplex ligation-dependent probe amplification (MLPA) assay for rapid detection of copy number variants in the genome","volume":"36","author":"Shen","year":"2009","journal-title":"J Genet Genomics"},{"key":"10.1016\/j.humpath.2013.04.026_bb0110","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.2353\/ajpath.2010.090901","article-title":"Increase in viral load, viral integration, and gain of telomerase genes during uterine cervical carcinogenesis can be simultaneously assessed by the HPV 16\/18 MLPA assay","volume":"177","author":"Theelen","year":"2010","journal-title":"Am J Pathol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0115","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/path.2070","article-title":"Genomic integration of oncogenic HPV and gain of the human telomerase gene TERC at 3q26 are strongly associated events in the progression of uterine cervical dysplasia to invasive cancer","volume":"210","author":"Hopman","year":"2006","journal-title":"J Pathol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0120","doi-asserted-by":"crossref","first-page":"3977","DOI":"10.1038\/sj.onc.1206629","article-title":"A comprehensive analysis of HPV integration loci in anogenital lesions combining transcript and genome-based amplification techniques","volume":"22","author":"Ziegert","year":"2003","journal-title":"Oncogene"},{"key":"10.1016\/j.humpath.2013.04.026_bb0125","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1002\/ijc.1174","article-title":"Overexpression of p16(INK4A) as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri","volume":"92","author":"Klaes","year":"2001","journal-title":"Int J Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0130","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1002\/ijc.20862","article-title":"HPV in situ hybridization: impact of different protocols on the detection of integrated HPV","volume":"115","author":"Hopman","year":"2005","journal-title":"Int J Cancer"},{"key":"10.1016\/j.humpath.2013.04.026_bb0135","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1177\/002215549704501013","article-title":"Sensitive multicolor fluorescence in situ hybridization using catalyzed reporter deposition (CARD) amplification","volume":"45","author":"Speel","year":"1997","journal-title":"J Histochem Cytochem"},{"key":"10.1016\/j.humpath.2013.04.026_bb0140","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1136\/jcp.44.12.990","article-title":"Episomal and integrated human papillomavirus in cervical neoplasia shown by non-isotopic in situ hybridisation","volume":"44","author":"Cooper","year":"1991","journal-title":"J Clin Pathol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0145","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/0076-6879(93)18026-9","article-title":"Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE","volume":"218","author":"Frohman","year":"1993","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0150","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1002\/path.2192","article-title":"Integration of high-risk human papillomavirus: a key event in cervical carcinogenesis?","volume":"212","author":"Pett","year":"2007","journal-title":"J Pathol"},{"key":"10.1016\/j.humpath.2013.04.026_bb0155","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1128\/JVI.61.4.962-971.1987","article-title":"Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines","volume":"61","author":"Baker","year":"1987","journal-title":"J Virol"}],"container-title":["Human Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0046817713002281?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0046817713002281?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T21:04:09Z","timestamp":1715893449000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0046817713002281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":31,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["S0046817713002281"],"URL":"https:\/\/doi.org\/10.1016\/j.humpath.2013.04.026","relation":{},"ISSN":["0046-8177"],"issn-type":[{"value":"0046-8177","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]}}}