{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T07:30:00Z","timestamp":1769931000754,"version":"3.49.0"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Human Pathology"],"published-print":{"date-parts":[[2021,11]]},"DOI":"10.1016\/j.humpath.2021.08.007","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T10:46:10Z","timestamp":1630061170000},"page":"108-114","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Effect of EDTA decalcification on estrogen receptor and progesterone receptor immunohistochemistry and HER2\/neu fluorescence in situ hybridization in breast carcinoma"],"prefix":"10.1016","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9551-2492","authenticated-orcid":false,"given":"Erik","family":"Washburn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Caruso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Walls","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.humpath.2021.08.007_bib1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.canep.2018.02.003","article-title":"Disparity in breast cancer mortality by age and geography in 10 racially diverse US cities","volume":"53","author":"Sighoko","year":"2018","journal-title":"Cancer Epidemiol"},{"key":"10.1016\/j.humpath.2021.08.007_bib2","doi-asserted-by":"crossref","first-page":"6243s","DOI":"10.1158\/1078-0432.CCR-06-0931","article-title":"Clinical features of metastatic bone disease and risk of skeletal morbidity","volume":"12","author":"Coleman","year":"2006","journal-title":"Clin Canc Res"},{"key":"10.1016\/j.humpath.2021.08.007_bib3","doi-asserted-by":"crossref","first-page":"3271","DOI":"10.1200\/JCO.2009.25.9820","article-title":"Metastatic behavior of breast cancer subtypes","volume":"28","author":"Kennecke","year":"2010","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.humpath.2021.08.007_bib4","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1200\/JCO.2015.61.1459","article-title":"Use of biomarkers to guide decisions on systemic therapy for women with metastatic breast cancer: American Society of clinical Oncology clinical practice guideline","volume":"33","author":"Van Poznak","year":"2015","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.humpath.2021.08.007_bib5","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.critrevonc.2012.05.002","article-title":"Soria JC Discrepancies between primary tumor and metastasis: a literature review on clinically established biomarkers","volume":"84","author":"Vignot","year":"2012","journal-title":"Crit Rev Oncol Hematol"},{"key":"10.1016\/j.humpath.2021.08.007_bib6","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.1200\/JCO.2012.42.6734","article-title":"Testing for discordance at metastatic relapse of breast cancer matters","volume":"30","author":"van Diest","year":"2012","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.humpath.2021.08.007_bib7","series-title":"National Comprehensive Cancer Network (NCCN) guideline version 1.2019 breast cancer","year":"2019"},{"key":"10.1016\/j.humpath.2021.08.007_bib8","doi-asserted-by":"crossref","first-page":"433","DOI":"10.6004\/jnccn.2017.0044","article-title":"NCCN guidelines insights: breast cancer, version 1.2017","volume":"15","author":"Gradishar","year":"2017","journal-title":"J Natl Compr Canc Netw"},{"key":"10.1016\/j.humpath.2021.08.007_bib9","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1097\/PAS.0000000000001321","article-title":"Decalcification of breast cancer bone metastases with EDTA does not affect ER, PR, and HER2 results","volume":"43","author":"van","year":"2019","journal-title":"Am J Surg Pathol"},{"key":"10.1016\/j.humpath.2021.08.007_bib10","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1097\/PAI.0000000000000277","article-title":"Effect of hydrochloric acid decalcification on expression pattern of prognostic markers in invasive breast carcinomas","volume":"25","author":"Maclary","year":"2017","journal-title":"Appl Immunohistochem Mol Morphol"},{"key":"10.1016\/j.humpath.2021.08.007_bib11","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1111\/j.1524-4741.2011.01168.x","article-title":"Impact of decalcification on receptor status in breast cancer","volume":"17","author":"Darvishian","year":"2011","journal-title":"Breast J"},{"key":"10.1016\/j.humpath.2021.08.007_bib12","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1038\/modpathol.2016.116","article-title":"Influence of decalcification procedures on immunohistochemistry and molecular pathology in breast cancer","volume":"29","author":"Schrijver","year":"2016","journal-title":"Mod Pathol"},{"key":"10.1016\/j.humpath.2021.08.007_bib13","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1177\/002215549904700512","article-title":"Effect of bone decalcification procedures on DNA in situ hybridization and comparative genomic hybridization. EDTA is highly preferable to a routinely used acid decalcifier","volume":"47","author":"Alers","year":"1999","journal-title":"J Histochem Cytochem"},{"key":"10.1016\/j.humpath.2021.08.007_bib14","doi-asserted-by":"crossref","first-page":"545","DOI":"10.5858\/arpa.2019-0904-SA","article-title":"Estrogen and progesterone receptor testing in breast cancer: American Society of Clinical Oncology\/College of American Pathologists guideline update","volume":"144","author":"Allison","year":"2020","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.humpath.2021.08.007_bib15","doi-asserted-by":"crossref","first-page":"236","DOI":"10.4132\/jptm.2015.03.16","article-title":"Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study","volume":"49","author":"Choi","year":"2015","journal-title":"J Pathol Transl Med"},{"key":"10.1016\/j.humpath.2021.08.007_bib16","first-page":"155","article-title":"Prognostic and predictive factors in breast cancer by immunohistochemical analysis","volume":"11","author":"Allred","year":"1998","journal-title":"Mod Pathol"},{"key":"10.1016\/j.humpath.2021.08.007_bib17","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1200\/JCO.1999.17.5.1474","article-title":"Estrogen receptor status by immunohistochemistry is superior to the ligand-binding assay for predicting response to adjuvant endocrine therapy in breast cancer","volume":"17","author":"Harvey","year":"1999","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.humpath.2021.08.007_bib18","doi-asserted-by":"crossref","first-page":"S34","DOI":"10.1007\/s00280-003-0592-1","article-title":"Clinical significance of the expression of estrogen receptors alpha and beta for endocrine therapy of breast cancer","volume":"52","author":"Iwase","year":"2003","journal-title":"Canc Chemother Pharmacol"},{"key":"10.1016\/j.humpath.2021.08.007_bib19","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1200\/JCO.2018.77.8738","article-title":"Human epidermal growth factor receptor 2 testing in breast cancer: American Society of clinical Oncology\/College of American Pathologists clinical practice guideline focused update","volume":"36","author":"Wolff","year":"2018","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.humpath.2021.08.007_bib20","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1136\/jcp.2008.059717","article-title":"HER-2\/neu analysis in breast cancer bone metastases","volume":"62","author":"Zustin","year":"2009","journal-title":"J Clin Pathol"},{"key":"10.1016\/j.humpath.2021.08.007_bib21","doi-asserted-by":"crossref","first-page":"dju055","DOI":"10.1093\/jnci\/dju055","article-title":"US incidence of breast cancer subtypes defined by joint hormone receptor and HER2 status","volume":"106","author":"Howlader","year":"2014","journal-title":"J Natl Canc Inst"}],"container-title":["Human Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0046817721001490?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0046817721001490?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T16:39:10Z","timestamp":1758645550000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0046817721001490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":21,"alternative-id":["S0046817721001490"],"URL":"https:\/\/doi.org\/10.1016\/j.humpath.2021.08.007","relation":{},"ISSN":["0046-8177"],"issn-type":[{"value":"0046-8177","type":"print"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effect of EDTA decalcification on estrogen receptor and progesterone receptor immunohistochemistry and HER2\/neu fluorescence in situ hybridization in breast carcinoma","name":"articletitle","label":"Article Title"},{"value":"Human Pathology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.humpath.2021.08.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}