{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:10:19Z","timestamp":1792789819451,"version":"4.5.0"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2016,8]]},"DOI":"10.1016\/j.ijbiomac.2016.04.086","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T13:04:59Z","timestamp":1462194299000},"page":"700-706","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":67,"special_numbering":"C","title":["Biosynthesis, purification and characterization of polyhydroxybutyrate from Botryococcus braunii k\u00fctz"],"prefix":"10.1016","volume":"89","author":[{"given":"Ganapathy","family":"Kavitha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chidambaram","family":"Kurinjimalar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnan","family":"Sivakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perumal","family":"Palani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramasamy","family":"Rengasamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0005","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1574-6976.2011.00304.x","article-title":"Microalgae in the postgenomic era: a blooming reservoir for new natural products","volume":"36","author":"Sasso","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0010","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1080\/10408410802086783","article-title":"A perspective on the biotechnological potential of microalgae","volume":"34","author":"Raja","year":"2008","journal-title":"Crit. Rev. Microbiol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0015","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.rser.2009.07.020","article-title":"Microalgae for biodiesel production and other applications","volume":"14","author":"Mata","year":"2010","journal-title":"Renew. Sust. Energy Rev."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0020","series-title":"Novel biodegradable microbial polymers","year":"1990"},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0025","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.procbio.2009.01.008","article-title":"Poly (3hydroxybutyrate) production by Cupriavidus necator using waste glycerol","volume":"44","author":"Joa","year":"2009","journal-title":"Process Biochem."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0030","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.jhazmat.2010.11.014","article-title":"Simultaneous methane production and wastewater reuse by a membrane \u2212 based process: evaluation with raw domestic wastewater","volume":"186","author":"Gao","year":"2011","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0035","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.tibtech.2007.12.002","article-title":"Biodiesel from microalgae beats bioethanol","volume":"26","author":"Chisti","year":"2008","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0040","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.biotechadv.2011.05.015","article-title":"Production and harvesting of microalgae for wastewater treatment biofuels, and bioproducts","volume":"29","author":"Christenson","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0045","doi-asserted-by":"crossref","first-page":"6","DOI":"10.4172\/2155-6199.1000e113","article-title":"Bioremediation of domestic wastewater and production of bioproducts from microalgae using waste stabilization ponds","volume":"3","author":"Rahman","year":"2012","journal-title":"J. Bioremed. Biodegrad."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0050","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1002\/bit.24451","article-title":"Rotating algal biofilm reactor and spool harvester for wastewater treatment with biofuels by-products","volume":"109","author":"Christenson","year":"2012","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0055","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1016\/j.biortech.2004.10.009","article-title":"Accumulation of poly-hydroxybutyrate in Nostoc muscorum: regulation by pH, light-dark cycles, N and P status and carbon sources","volume":"96","author":"Sharma","year":"2005","journal-title":"Bioresour. Technol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0060","volume":"I","author":"Fritsch","year":"1935"},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0065","unstructured":"G. Mahendraperumal, N. Anand. Manual of fresh water algae of Tamil Nadu. Bishen Singh, Mahendrapal Singh, 23-A, Dehra Dun. (2009)."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0070","series-title":"Physiological Studies, Morphological and Taxonomical Investigation of Nostoc and Anabaena in Culture","author":"Kanz","year":"1969"},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0075","first-page":"493","article-title":"Lipid composition of a green alga: Botryococcus braunii","volume":"51","author":"Yamaguchi","year":"1987","journal-title":"Agric. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0080","series-title":"Methods in Enzymology","doi-asserted-by":"crossref","DOI":"10.1016\/0076-6879(87)48036-1","article-title":"Chlorophylls and carotenoids: pigments of photosynthetic biomembranes","author":"Lichtenthaler","year":"1987"},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0085","first-page":"305","article-title":"Calorimetric methods for determination of sugars and related substance","volume":"28","author":"Dubois","year":"1956","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0090","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipids from animal tissues","volume":"226","author":"Folch","year":"1956","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0095","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1078\/0944-5013-00161","article-title":"Poly-hydroxybutyrate (PHB) synthesis by recombinant Escherichia coli harbouring, streptomyces aureoficiens PHB biosynthesis genes: effects of various carbon and nitrogen sources","volume":"158","author":"Mahishi","year":"2003","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0100","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1128\/jb.82.1.33-36.1961","article-title":"Assay of poly-hydroxybutyric acid","volume":"82","author":"John","year":"1961","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0105","first-page":"52","article-title":"Assay of poly\u2013hydroxybutyric acid","volume":"82","author":"Law","year":"1969","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0110","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/BF02815620","article-title":"Polyhydroxybutyrate production by Azotobacter chroococcum","volume":"46","author":"Parshad","year":"2001","journal-title":"Folia Microbiol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0115","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.bej.2006.11.021","article-title":"Poly3(hydroxyalkanoates) produced from oily substrates by Pseudomonas aeruginosa 47T2 (NCBIM 40044): effect of nutrients and incubation temperature on polymer composition","volume":"35","author":"Haba","year":"2007","journal-title":"Biochem. Enginee. J."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0120","doi-asserted-by":"crossref","first-page":"18","DOI":"10.5897\/AJB2004.000-2004","article-title":"Production of polyhydroxyalkanoates a bacterial biodegradable polymer","volume":"3","author":"Ojumun","year":"2004","journal-title":"Afr. J. Biotechnol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0125","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1128\/MMBR.63.1.21-53.1999","article-title":"Metabolic engineering of poly\u2013hydroxy alkanoates from DNA to plastic","volume":"3","author":"Madison","year":"1999","journal-title":"Microbiol. Mol. Bio. Rev."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0130","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s10811-011-9663-6","article-title":"PHB accumulation in Nostoc muscorum under different carbon stress situations","volume":"24","author":"Hase","year":"2011","journal-title":"J. Appl. Phycol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0135","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1574-6968.1992.tb05835.x","article-title":"Poly(hydroxyalkanoates) in cyanobacteria: a review","volume":"103","author":"Stal","year":"1992","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0140","first-page":"308","article-title":"The occurrance of poly-b-hydroxybutyrate in the blue\u2013green alga: Chlorogloea fritschii","volume":"120","author":"Carr","year":"1966","journal-title":"Biochem. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0145","first-page":"2","article-title":"Investigation of the poly-\u03b2-hydroxybutyrate (PHB) producing in mountain bacterial strains by transmission electron microscopy","volume":"16","author":"Carpa","year":"2011","journal-title":"Rom. Biotech. Lett."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0150","doi-asserted-by":"crossref","first-page":"9765","DOI":"10.1016\/j.biortech.2010.07.089","article-title":"Production and characterization of a biodegradable poly(hydroxybutyrate-co-hydroxyvalerate) (PHB-co-PHV) copolymer by moderately haloalkalitolerant Halomonas campisalis MCM B-1027 isolated from Lonar Lake, India","volume":"101","author":"Kulkarni","year":"2010","journal-title":"Biores. Technol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0155","first-page":"276","article-title":"Synthesis and characterization of PHB from Bacillus thuringienesis R1","volume":"5","author":"Rohini","year":"2006","journal-title":"Ind. J. Biotechnol."},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0160","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.polymer.2009.02.025","article-title":"Change of structure and free volume properties of semi-crystalline poly (3-hydroxybutyrate-cohydroxyvaletae) duringthermal treatments by positron annihilation lifetime","volume":"50","author":"Cheng","year":"2009","journal-title":"Polymer"},{"key":"10.1016\/j.ijbiomac.2016.04.086_bib0165","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1002\/pi.2431","article-title":"Synthesis, characterization and biocompatibility of novel biodegradable poly[((R)-3-hydroxybutyrate)-block-(d, l- lactide)-block-(epsilon-aprolactone)] triblock copolymers","volume":"57","author":"Wu","year":"2008","journal-title":"Polym. Int."}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813016304032?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813016304032?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:36:58Z","timestamp":1761489418000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141813016304032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8]]},"references-count":33,"alternative-id":["S0141813016304032"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2016.04.086","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2016,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biosynthesis, purification and characterization of polyhydroxybutyrate from Botryococcus braunii k\u00fctz","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2016.04.086","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}