{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:30:56Z","timestamp":1791307856172,"version":"4.3.1"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T00:00:00Z","timestamp":1500336000000},"content-version":"vor","delay-in-days":259,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Pesquisa Translacional em Sa\u00fade e Ambiente na Regi\u00e3o Amaz\u00f4nica"},{"DOI":"10.13039\/501100004933","name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Toxinas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004933","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Rond\u00f4nia"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2016,11]]},"DOI":"10.1016\/j.ijbiomac.2016.07.022","type":"journal-article","created":{"date-parts":[[2016,7,8]],"date-time":"2016-07-08T00:11:26Z","timestamp":1467936686000},"page":"329-337","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":34,"special_numbering":"C","title":["Mechanism of the cytotoxic effect of l-amino acid oxidase isolated from Bothrops alternatus snake venom"],"prefix":"10.1016","volume":"92","author":[{"given":"Patr\u00edcia H.","family":"Ribeiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana P.","family":"Zuliani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla F.C.","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo A.","family":"Calderon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo G.","family":"St\u00e1beli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auro","family":"Nomizo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreimar M.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0005","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/S0041-0101(02)00102-2","article-title":"Snake venom L-amino acid oxidases","volume":"40","author":"Du","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0010","doi-asserted-by":"crossref","first-page":"908","DOI":"10.2174\/092986609788923347","article-title":"Snake venom L-amino acid oxidases: some consideration about their functional characterization","volume":"16","author":"Zuliani","year":"2009","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0015","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.toxicon.2012.05.001","article-title":"Past decade study of snake venom L-amino acid oxidase","volume":"60","author":"Guo","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0020","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1155\/2014\/196754","article-title":"Snake venom L-amino acid oxidases: trends in pharmacology and biochemistry","volume":"2014","author":"Izidoro","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0025","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0167-4838(00)00229-6","article-title":"Molecular characterization of L-amino acid oxidase from Agkistrodon halys blomhoffii with special reference to platelet aggregation","volume":"1544","author":"Takatsuka","year":"2001","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0030","first-page":"937","article-title":"Isolation and characterization of an unusual form of L-amino acid oxidase from King cobra (Ophiophagus hannah) venom","volume":"19","author":"Tan","year":"1989","journal-title":"Biochem. Int."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0035","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1016\/0041-0101(94)90407-3","article-title":"Purification and characterization of L-amino acid oxidase from king cobra (Ophiophagus hannah) venom and its effects on human platelet aggregation","volume":"32","author":"Li","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0040","doi-asserted-by":"crossref","first-page":"3197","DOI":"10.1021\/bi992416z","article-title":"Molecular cloning and functional analysis of apoxin I, a snake venom-derived apoptosis inducing factor with L-amino acid oxidase activity","volume":"39","author":"Torii","year":"2000","journal-title":"Biochemistry"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0045","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/S1357-2725(97)00024-1","article-title":"Characterization and cytotoxicity of L-amino acid oxidase from the venom of king cobra (Ophiophagus Hannah)","volume":"29","author":"Ahn","year":"1997","journal-title":"Int. J. Biochem. Cell. Biol."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0050","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1248\/bpb.21.924","article-title":"Characterization of an apoptosis-inducing factor in habu snake venom as a glycyrrhizin (GL)-binding protein potently inhibited by GL in vitro","volume":"21","author":"Abe","year":"1998","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0055","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1006\/abbi.1999.1287","article-title":"Isolation and structural characterization of a cytotoxic L-amino acid oxidase from Agkistrodon contortrix laticinctus snake venom: preliminary crystallographic data","volume":"368","author":"Souza","year":"1999","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0060","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1006\/abbi.2000.2130","article-title":"Isolation structural, and functional characterization of an apoptosis-inducing L-amino acid oxidase from leaf-nosed viper (Eristocophis macmahoni) snake venom","volume":"384","author":"Ali","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0065","doi-asserted-by":"crossref","first-page":"4204","DOI":"10.1093\/emboj\/19.16.4204","article-title":"The structure of L-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site","volume":"19","author":"Pawelek","year":"2000","journal-title":"EMBO J."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0070","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.cbpa.2008.07.007","article-title":"Evidence of caspase-mediated apoptosis induced by l-amino acid oxidase isolated from Bothrops atrox snake venom","volume":"151","author":"Alves","year":"2008","journal-title":"Comp. Biochem. Physiol."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0075","first-page":"91","article-title":"The research of l-amino acid oxidase in snake venom","volume":"8","author":"Huang","year":"2009","journal-title":"World J. Tumor"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0080","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.bbapap.2006.01.021","article-title":"Isolation and characterization of an apoptotic and platelet aggregation inhibiting L-amino acid oxidase from Vipera berus berus (common viper) venom","volume":"1764","author":"Samel","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0085","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1678-9199-20-23","article-title":"Snake venom L-amino acid oxidases: an overview on their antitumor effects","volume":"20","author":"Costa","year":"2014","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0090","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1006\/bbrc.1996.0996","article-title":"Identification of the snake venom substance that induces apoptosis","volume":"224","author":"Suhr","year":"1996","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0095","doi-asserted-by":"crossref","first-page":"9539","DOI":"10.1074\/jbc.272.14.9539","article-title":"Apoxin I a novel apoptosis-inducing factor with L-amino acid oxidase activity purified from western diamondback rattlesnake venom","volume":"272","author":"Torii","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0100","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1006\/bbrc.1993.1023","article-title":"Induction of apoptosis by hemorrhagic snake venom in vascular endothelial cells","volume":"190","author":"Araki","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0105","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1016\/j.bmc.2004.03.049","article-title":"Platelet aggregation and antibacterial effects of an L-amino acid oxidase purified from Bothrops alternatus snake venom","volume":"12","author":"St\u00e1beli","year":"2004","journal-title":"Bioorg. M\u00e9d. Chem."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0110","first-page":"612","article-title":"Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader","volume":"27","author":"Hong","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0115","doi-asserted-by":"crossref","first-page":"7034","DOI":"10.1016\/j.bmc.2006.06.025","article-title":"Biochemical and functional characterization of an l-amino acid oxidase isolated from Bothrops pirajai snake venom","volume":"14","author":"Izidoro","year":"2006","journal-title":"Bioorg. Med. Chem."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0120","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.ijbiomac.2007.01.006","article-title":"Cytotoxic l-amino acid oxidase from Bothrops moojeni: biochemical and functional characterization","volume":"41","author":"Stabeli","year":"2007","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0125","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.toxicon.2013.12.013","article-title":"Effect of L-amino acid oxidase from Calloselasma rhodosthoma snake venom on human neutrophils","volume":"80","author":"Pontes","year":"2014","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0130","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.ijbiomac.2015.07.004","article-title":"Evaluating the microbicidal, antiparasitic and antitumor effects of CR-LAAO from Calloselasma rhodostoma venom","volume":"80","author":"Costa","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0135","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.toxicon.2015.08.007","article-title":"L-amino acid oxidase from Bothrops marajoensis causes nephrotoxicity in isolated perfused kidney and cytotoxicity in MDCK renal cells","volume":"104","author":"Dantas","year":"2015","journal-title":"Toxicon"},{"issue":"5 Suppl","key":"10.1016\/j.ijbiomac.2016.07.022_bib0140","doi-asserted-by":"crossref","first-page":"613S","DOI":"10.1080\/07315724.2007.10719667","article-title":"Mechanisms and significance of cell volume regulation","author":"Lang","year":"2007","journal-title":"J. Am. Coll. Nutr."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0145","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1146\/annurev.pharmtox.41.1.367","article-title":"Cellular responses to DNA damage","volume":"41","author":"Norbury","year":"2001","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0150","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1080\/01926230701320337","article-title":"Apoptosis: a review of programmed cell death","volume":"35","author":"Elmore","year":"2007","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0155","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1007\/s10495-015-1157-6","article-title":"Apoptosis induction in human breast cancer (MCF-7) cells by a novel venom L-amino acid oxidase (Rusvinoxidase) is independent of its enzymatic activity and is accompanied by caspase-7 activation and reactive oxygen species production","volume":"20","author":"Mukherjee","year":"2015","journal-title":"Apoptosis"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0160","doi-asserted-by":"crossref","first-page":"e0132569","DOI":"10.1371\/journal.pone.0132569","article-title":"L-Aminoacid oxidase from Bothrops leucurus venom induces nephrotoxicity via apoptosis and necrosis","volume":"10","author":"Morais","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0165","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1002\/jps.20874","article-title":"Oxidative stress and apoptosis: impact on cancer therapy","volume":"96","author":"Ozben","year":"2007","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0170","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1042\/bj3330291","article-title":"The cellular response to oxidative stress: influences of mitogen-activated protein kinase signalling pathways on cell survival","volume":"333","author":"Wang","year":"1998","journal-title":"Biochem. J."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0175","doi-asserted-by":"crossref","first-page":"7013","DOI":"10.1074\/jbc.272.11.7013","article-title":"Selective activation of caspases during apoptotic induction in HL-60 cells. Effects of a tetrapeptide inhibitor","volume":"272","author":"Polverino","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2016.07.022_bib0180","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1248\/bpb.26.964","article-title":"Diethyldithiocarbamate-induced cytotoxicity and apoptosis in leukemia cell lines","volume":"26","author":"Kanno","year":"2003","journal-title":"Biol. Pharm. Bull."}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813016307565?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813016307565?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T05:24:01Z","timestamp":1566451441000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141813016307565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11]]},"references-count":36,"alternative-id":["S0141813016307565"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2016.07.022","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2016,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mechanism of the cytotoxic effect of l-amino acid oxidase isolated from Bothrops alternatus snake venom","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2016.07.022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V.","name":"copyright","label":"Copyright"}]}}