{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T21:19:49Z","timestamp":1768771189384,"version":"3.49.0"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1016\/j.ijbiomac.2019.09.032","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T02:13:12Z","timestamp":1567649592000},"page":"1044-1054","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom"],"prefix":"10.1016","volume":"141","author":[{"given":"Frank Denis","family":"Torres-Huaco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Maru\u00f1ak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Teibler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soledad","family":"Bustillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ofelia","family":"Acosta de P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Cristina","family":"Leiva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Alberto","family":"Ponce-Soto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Marangoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0005","doi-asserted-by":"crossref","first-page":"290","DOI":"10.3390\/toxins9090290","article-title":"A review and database of snake venom proteomes","volume":"9","author":"Tasoulis","year":"2017","journal-title":"Toxins"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0010","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3390\/toxins8050155","article-title":"ADAM and ADAMTS family proteins and snake venom metalloproteinases: a structural overview","volume":"8","author":"Takeda","year":"2016","journal-title":"Toxins"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0015","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.toxicon.2012.09.004","article-title":"Snake venom metalloproteinases","volume":"62","author":"Markland","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0020","doi-asserted-by":"crossref","first-page":"E304","DOI":"10.3390\/toxins8100304","article-title":"A comprehensive view of the structural and functional alterations of extracellular matrix by snake venom metalloproteinases (SVMPs): novel perspectives on the pathophysiology of envenoming","volume":"8","author":"Guti\u00e9rrez","year":"2016","journal-title":"Toxins"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0025","doi-asserted-by":"crossref","first-page":"E392","DOI":"10.3390\/toxins9120392","article-title":"Direct fibrinolytic snake venom metalloproteinases affecting hemostasis: structural, biochemical features and therapeutic potential","volume":"9","author":"Sanchez","year":"2017","journal-title":"Toxins"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0030","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1590\/S0074-02762005000900031","article-title":"Inflammatory effects of snake venom metalloproteinases","volume":"100","author":"Teixeira","year":"2005","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0000727","article-title":"Mechanisms of vascular damage by hemorrhagic snake venom metalloproteinases: tissue distribution and in situ hydrolysis","volume":"4","author":"Baldo","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0040","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1016\/j.jprot.2011.03.026","article-title":"Key events in microvascular damage induced by snake venom hemorrhagic metalloproteinases","volume":"74","author":"Escalante","year":"2011","journal-title":"J. Proteomics."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003731","article-title":"Tissue localization and extracellular matrix degradation by PI, PII and PIII snake venom metalloproteinases: clues on the mechanisms of venom-induced hemorrhage","volume":"9","author":"Herrera","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0050","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0300-9084(01)01282-2","article-title":"Pathological alterations induced by neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi snake venom","volume":"83","author":"Rodrigues","year":"2001","journal-title":"Biochimie"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0055","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.cbpc.2010.11.008","article-title":"Purification and functional characterization of a new metalloproteinase (BleucMP) from Bothrops leucurus snake venom","volume":"153","author":"Gomes","year":"2011","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0060","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/352420","article-title":"Rapid purification of a new P-I class metalloproteinase from Bothrops moojeni venom with antiplatelet activity","author":"de Queiroz","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0065","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.toxicon.2007.11.017","article-title":"Isolation and structural characterization of a new fibrin(ogen)olytic metalloproteinase from Bothrops moojeni snake venom","volume":"51","author":"Bernardes","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0070","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.toxicon.2008.10.007","article-title":"BthMP: a new weakly hemorrhagic metalloproteinase from Bothrops moojeni snake venom","volume":"53","author":"Gomes","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0075","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s10930-010-9267-z","article-title":"Purification and characterization of a new weak hemorrhagic metalloproteinase BmHF-1 from Bothrops marajoensis snake venom","volume":"29","author":"Torres-Huaco","year":"2010","journal-title":"Protein J."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0080","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.toxicon.2010.06.016","article-title":"Isolation and biological characterization of Batx-I, a weak hemorrhagic and fibrinogenolytic PI metalloproteinase from Colombian Bothrops atrox venom","volume":"56","author":"Pati\u00f1o","year":"2010","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0085","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/S0041-0101(98)00268-2","article-title":"Characterization of the local tissue damage induced by LHF-II, a metalloproteinase with weak hemorrhagic activity isolated from Lachesis muta muta snake venom","volume":"37","author":"Rucavado","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0090","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.toxicon.2015.09.005","article-title":"BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties","volume":"106","author":"Kayano","year":"2015","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0095","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.toxicon.2007.08.005","article-title":"BnP1, a novel P-I metalloproteinase from Bothrops neuwiedi venom: biological effects benchmarking relatively to jararhagin, a P-III SVMP","volume":"51","author":"Baldo","year":"2008","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0100","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.toxicon.2007.03.002","article-title":"Cytotoxic, thrombolytic and edematogenic activities of leucurolysin-a, a metalloproteinase from Bothrops leucurus snake venom","volume":"50","author":"Gremski","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0105","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.2174\/138161207782023711","article-title":"Importance of snake venom metalloproteinases in cell biology: effects on platelets, inflammatory and endothelial cells","volume":"13","author":"Moura-da-Silva","year":"2007","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0110","series-title":"The Venomous Reptiles of the Western Hemisphere","volume":"2 Vol.","author":"Campbell","year":"2004"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0115","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/0041-0101(96)00016-5","article-title":"Biochemical and biological characterization of Ecuadorian pitviper venoms (genera Bothriechis, Bothriopsis, Bothrops and Lachesis)","volume":"34","author":"Kuch","year":"1996","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0120","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.toxicon.2007.03.020","article-title":"Biochemical and biological characterization of the venoms of Bothriopsis bilineata and Bothriopsis taeniata (Serpentes: Viperidae)","volume":"50","author":"Porto","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0125","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1590\/S0037-86822009000300020","article-title":"First report of an accident with the speckled forest pit viper (Bothriopsis taeniata) in Brazil","volume":"42","author":"Torrez","year":"2009","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0130","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jprot.2015.10.020","article-title":"Revealing the functional structure of a new PLA2 K49 from Bothriopsis taeniata snake venom employing automatic \u201cde novo\u201d sequencing using CID\/HCD\/ETD MS\/MS analyses","volume":"131","author":"Carregari","year":"2016","journal-title":"J. Proteomics."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0135","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0140","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0145","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.toxicon.2012.06.010","article-title":"LmrTX, a basic PLA\u2082 (D49) purified from Lachesis muta rhombeata snake venom with enzymatic-related antithrombotic and anticoagulant activity","volume":"60","author":"Damico","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0150","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1055\/s-0037-1613061","article-title":"Purification and characterization of a novel metalloproteinase, acurhagin, from Agkistrodon acutus venom","volume":"87","author":"Wang","year":"2002","journal-title":"Thromb. Haemost."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0155","doi-asserted-by":"crossref","first-page":"4159","DOI":"10.1128\/iai.58.12.4159-4162.1990","article-title":"Purification and characterization of a protease produced by Vibrio mimicus","volume":"58","author":"Chowdhury","year":"1990","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0160","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/903292","article-title":"Rapid purification and procoagulant and platelet aggregating activities of rhombeobin: a thrombin-like\/gyroxin-like enzyme from Lachesis muta rhombeata snake venom","author":"Torres-Huaco","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0165","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0003-2697(84)90307-5","article-title":"Amino acid analysis by reverse-phase high performance liquid chromatography: precolumn derivatization with phenylisothiocyanate","volume":"136","author":"Heinrikson","year":"1984","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0170","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/0041-0101(85)90380-0","article-title":"Neutralization of proteolytic and hemorrhagic activities of Costa Rican snake venom by a polyvalent antivenom","volume":"23","author":"Guti\u00e9rrez","year":"1985","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0175","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.toxicon.2011.11.007","article-title":"Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells","volume":"59","author":"Bustillo","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0180","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0024-3205(01)01432-1","article-title":"Cytotoxicity and apoptosis produced by troglitazone in human hepatoma cells","volume":"70","author":"Yamamoto","year":"2001","journal-title":"Life Sci."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0185","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0041-0101(98)00171-8","article-title":"Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro","volume":"37","author":"Lomonte","year":"1999","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0190","first-page":"509","article-title":"Mice plasma fibrinogen consumption by thrombin-like enzyme present in rattlesnake venom from the north-east region of Argentina","volume":"64","author":"Maru\u00f1ak","year":"2004","journal-title":"Medicina"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0195","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.toxicon.2008.10.010","article-title":"Systemic alterations induced by a Bothrops alternatus hemorrhagic metalloproteinase (baltergin) in mice","volume":"53","author":"Gay","year":"2009","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0200","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0041-0101(94)00138-X","article-title":"Isolation and characterization of a metalloproteinase with weak hemorrhagic activity from the venom of the snake Bothrops asper (terciopelo)","volume":"33","author":"Guti\u00e9rrez","year":"1995","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0205","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.cbpc.2005.11.005","article-title":"Snake venom metalloproteases: structure and function of catalytic and disintegrin domains","volume":"142","author":"Ramos","year":"2006","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0210","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.toxicon.2011.01.006","article-title":"Haemostatically active proteins in snake venoms","volume":"57","author":"Sajevic","year":"2011","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0215","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/S0300-9084(00)01163-9","article-title":"Snake venom metalloproteinases: their role in the pathogenesis of local tissue damage","volume":"82","author":"Guti\u00e9rrez","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0220","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.ijbiomac.2014.08.050","article-title":"Biochemical properties of a new PI SVMP from Bothrops pauloensis: inhibition of cell adhesion and angiogenesis","volume":"72","author":"Ach\u00ea","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0225","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1016\/j.bbagen.2012.07.011","article-title":"BJ-PI2, a non-hemorrhagic metalloproteinase from Bothrops jararaca snake venom","volume":"1820","author":"da Silva","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0230","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1124\/mol.107.038018","article-title":"Antithrombotic effect of a protein-type I class snake venom metalloproteinase, kistomin, is mediated by affecting glycoprotein Ib-von Willebrand factor interaction","volume":"72","author":"Hsu","year":"2007","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.ijbiomac.2019.09.032_bb0235","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.ijbiomac.2016.11.063","article-title":"Antithrombotic activity of Batroxase, a metalloprotease from Bothrops atrox venom, in a model of venous thrombosis","volume":"95","author":"Jacob-Ferreira","year":"2017","journal-title":"Int. J. Biol. Macromol."}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813019347154?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813019347154?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T07:44:48Z","timestamp":1758872688000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141813019347154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":47,"alternative-id":["S0141813019347154"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2019.09.032","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Local and systemic effects of BtaMP-1, a new weakly hemorrhagic Snake Venom Metalloproteinase purified from Bothriopsis taeniata Snake Venom","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2019.09.032","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}