{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:18:40Z","timestamp":1788297520653,"version":"build-2803163510"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1016\/j.ijbiomac.2020.07.190","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T10:50:25Z","timestamp":1595242225000},"page":"1112-1123","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Molecular cloning and functional characterization of recombinant Loxtox from Loxosceles similis venom"],"prefix":"10.1016","volume":"164","author":[{"given":"Hort\u00eansia Gomes","family":"Leal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e1rbara Bruna Ribeiro","family":"de Oliveira Mendes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Campolina Rebello","family":"Horta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00fabia Braga","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas Sales Medina","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Soares","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Lago","family":"Biscoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Kalapothakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo A.","family":"Machado de Avila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Ch\u00e1vez-Ol\u00f3rtegui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evanguedes","family":"Kalapothakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0005","author":"Minist\u00e9rio da Sa\u00fade"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0010","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s40409-017-0097-8","article-title":"Highlights in the knowledge of brown spider toxins","volume":"23","author":"Chaves-Moreira","year":"2017","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0015","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.toxicon.2019.11.002","article-title":"From taxonomy to molecular characterization of brown spider venom: an overview focused on Loxosceles similis","volume":"173","author":"Oliveira-Mendes","year":"2020","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0020","first-page":"113","article-title":"Sobre a presen\u00e7a de tr\u00eas esp\u00e9cies de Loxosceles Heineken & Lowe (Araneae: Sicariidae) no munic\u00edpio de Belo Horizonte, Minas Gerais, Brasil","volume":"5","author":"Machado","year":"2005","journal-title":"Lundiana."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0025","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.toxicon.2005.09.002","article-title":"Characterization of the venom from the Brazilian Brown Spider Loxosceles similis Moenkhaus, 1898 (Araneae, Sicariidae)","volume":"46","author":"Silvestre","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0030","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.toxicon.2012.03.007","article-title":"Cutaneous loxoscelism caused by Loxosceles similis venom and neutralization capacity of its specific antivenom","volume":"60","author":"Chatzaki","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0035","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1016\/S0140-6736(10)62230-1","article-title":"Spider bite","volume":"378","author":"Ibister","year":"2011","journal-title":"Lancet"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0040","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.toxicon.2007.07.001","article-title":"The Loxtox protein family in Loxosceles intermedia (Mello-Leitao) venom","volume":"50","author":"Kalapothakis","year":"2007","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0045","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.bbalip.2010.11.005","article-title":"Phospholipase-D activity and inflammatory response induced by brown spider dermonecrotic toxin: endothelial cell membrane phospholipids as targets for toxicity","volume":"1811","author":"Chaim","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0050","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.toxicon.2006.05.005","article-title":"The efficacy of antivenom in loxoscelism treatment","volume":"48","author":"Pauli","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0055","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1016\/j.vaccine.2009.04.038","article-title":"An in vivo protective response against toxic effects of the dermonecrotic protein from Loxosceles intermedia spider venom elicited by synthetic epitopes","volume":"27","author":"Felicori","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0060","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.toxicon.2006.04.010","article-title":"North and South American Loxosceles spiders: development of a polyvalent antivenom with recombinant sphingomyelinases D as antigens","volume":"48","author":"Olvera","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0065","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.toxicon.2014.05.007","article-title":"Innovative immunization protocols using chimeric recombinant protein for the production of polyspecific loxoscelic antivenom in horses","volume":"86","author":"Figueiredo","year":"2014","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0070","doi-asserted-by":"crossref","first-page":"653","DOI":"10.3389\/fimmu.2018.00653","article-title":"Recombinant protein containing B-cell epitopes of different Loxosceles spider toxins generates neutralizing antibodies in immunized rabbits","volume":"9","author":"Lima","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0075","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3390\/toxins11020108","article-title":"Design and production of a recombinant hybrid toxin to raise protective antibodies against Loxosceles spider venom","volume":"11","author":"Calabria","year":"2019","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0080","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1038\/jid.2013.40","article-title":"Lysophosphatidic acid mediates the release of cytokines and chemokines by human fibroblasts treated with loxosceles spider venom","volume":"133","author":"Horta","year":"2013","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0085","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.toxicon.2014.04.002","article-title":"Whole venom of Loxosceles similis activates caspases-3, -6, -7,and -9 in human primary skin fibroblasts","volume":"84","author":"Dantas","year":"2014","journal-title":"Toxicon."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0090","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1590\/S1678-91992012000300005","article-title":"Histopathological characterization of experimentally induced cutaneous loxoscelism in rabbits inoculated with Loxosceles similis venom","volume":"18","author":"Pereira","year":"2012","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0095","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.toxicon.2014.03.003","article-title":"Apoptosis, mast cell degranulation and collagen breakdown in the pathogenesis of loxoscelism in subcutaneously implanted sponges","volume":"84","author":"Pereira","year":"2014","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0100","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.toxicon.2016.08.002","article-title":"Description of Loxtox protein family and identification of a new group of phospholipases D from Loxosceles similis venom gland","volume":"120","author":"Dantas","year":"2016","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0105","doi-asserted-by":"crossref","first-page":"2693","DOI":"10.1371\/journal.pntd.0002693","article-title":"Molecular, immunological, and biological characterization of Tityus serrulatus venom hyaluronidase: new insights into its role in envenomation","volume":"8","author":"Horta","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0110","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1016\/j.biochi.2013.06.002","article-title":"Cloning, expression and characterization of a phospholipase D from Loxosceles gaucho venom gland","volume":"95","author":"Magalh\u00e3es","year":"2013","journal-title":"Biochime"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0115","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1042\/BJ20050043","article-title":"Brown recluse spider (Loxosceles reclusa) venom phospholipase D (PLD) generates lysophosphatidic acid (LPA)","volume":"391","author":"Lee","year":"2005","journal-title":"Biochem. J."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0120","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/j.biochi.2006.02.008","article-title":"Molecular cloning and functional characterization of two isoforms of dermonecrotic toxin from Loxosceles intermedia (brown spider) venom gland","volume":"88","author":"Silveira","year":"2006","journal-title":"Biochime"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.toxicon.2004.06.013","article-title":"Genetic and enzymatic characterization of sphingomyelinase D isoforms from the North American fiddleback spiders Loxosceles boneti and Loxosceles reclusa","volume":"44","author":"Ramos-Cerrillo","year":"2004","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0130","doi-asserted-by":"crossref","first-page":"10994","DOI":"10.1074\/jbc.M115.636951","article-title":"Variable substrate preference among phospholipase D toxins from Sicariid spiders","volume":"290","author":"Lajoie","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0135","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0140","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/0264-410X(91)90012-U","article-title":"In vivo protection against scorpion toxins by liposomal immunization","volume":"9","author":"Ch\u00e1vez-Ol\u00f3rtegui","year":"1991","journal-title":"Vaccine"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0145","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0022-1759(02)00140-0","article-title":"Application of the spot method to the identification of peptides and amino acids from the antibody paratope that contribute to antigen binding","volume":"267","author":"Laune","year":"2002","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0150","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0155","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/nar\/gkn750","article-title":"The SWISS-MODEL repository and associated resources","volume":"37","author":"Kiefer","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0160","article-title":"Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom","volume":"67","author":"Ullah","year":"2011","journal-title":"Acta Crystallogaphica Sect. F Struct. Biol. Cryst. Commun."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0165","author":"Delano"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0170","doi-asserted-by":"crossref","DOI":"10.1007\/s00705-002-0903-6","article-title":"Apoptosis in canine distemper","volume":"148","author":"Moro","year":"2003","journal-title":"Arch. Virol."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0175","series-title":"R Package Version 1.4.1","article-title":"nlme: linear and nonlinear mixed effects models","author":"Pinheiro","year":"2019"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0180","series-title":"R Package Version 1.4.1","article-title":"Emmeans: Estimated marginal means, aka least-squares means","author":"Lenth","year":"2019"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0185","author":"R.C. Team"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0190","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.bbrc.2006.01.123","article-title":"Structural insights into the catalytic mechanism of sphingomyelinases D and evolutionary relationship to glycerophosphodiester phosphodiesterases","volume":"342","author":"Murakami","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0195","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12862-018-1302-2","article-title":"Evolutionary dynamics of origin and loss in the deep history of phospholipase D toxin genes","volume":"18","author":"Cordes","year":"2018","journal-title":"BMC Evol. Biol."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0200","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.bbalip.2016.05.009","article-title":"Active site mapping of Loxosceles phospholipases D: biochemical and biological features","volume":"1861","author":"Vuitika","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0205","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0079240","article-title":"Identification of new sphingomyelinases D in pathogenic fungi and other pathogenic organisms","volume":"8","author":"Dias-Lopes","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0210","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002206","article-title":"A novel Hyaluronidase from Brown spider (Loxosceles intermedia) venom (Dietrich\u2019s Hyaluronidase): from cloning to functional characterization","volume":"7","author":"Ferrer","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0215","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.bbrc.2004.11.146","article-title":"Conformational changes of Loxosceles venom sphingomyelinases monitored by circular dichroism","volume":"327","author":"Andrade","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0220","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.toxicon.2005.12.005","article-title":"Kinetic and mechanistic characterization of the Sphingomyelinase D from Loxosceles intermedia spider venom","volume":"47","author":"Andrade","year":"2006","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0225","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.toxicon.2015.10.008","article-title":"Spider, bacterial and fungal phospholipase D toxins make cyclic phosphate products","volume":"108","author":"Lajoie","year":"2015","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0230","first-page":"27","article-title":"Loxosceles spiders (Araneae: Sicariidae) preying on invertebrates in Brazilian caves","volume":"6","author":"Souza-Silva","year":"2014","journal-title":"Speleobiology Notes."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0235","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0022-2011(84)90046-6","article-title":"Hemolymph pH of the larvae of three species of mosquitoes, and the effect of Romanomermis culicivorax parasitism on the blood pH of Culex pipiens","volume":"44","author":"Giblin","year":"1984","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0240","first-page":"431","article-title":"Physico-chemical properties of haemolymph of Chilopoda and Diplopoda (Myriapoda, Arthopoda): protein content, pH, osmolarity","volume":"81","author":"Xylander","year":"2009","journal-title":"Soil Org."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0245","doi-asserted-by":"crossref","first-page":"3062","DOI":"10.1242\/jeb.053991","article-title":"Regulation of gas exchange and haemolymph pH in the cockroach Nauphoeta cinerea","volume":"214","author":"Mattheus","year":"2011","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0250","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.toxicon.2005.03.024","article-title":"Localization of epitopes in the toxins of Tityus serrulatus scorpions and neutralizing potential of therapuetics antivenoms","volume":"46","author":"Maria","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0255","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.toxicon.2013.07.007","article-title":"Synthetic peptides for in vitro evaluation of the neutralizing potency of Loxosceles antivenoms","volume":"73","author":"Ramada","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0260","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1016\/j.vaccine.2013.03.048","article-title":"Generation and characterization of a recombinant chimeric protein (rCpLi) consisting of B-cell epitopes of a dermonecrotic protein from Loxosceles intermedia spider venom","volume":"31","author":"Mendes","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0265","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0121673","article-title":"Dissecting antibodies with regards to linear and conformational epitopes","volume":"10","author":"Forstr\u00f6m","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.ijbiomac.2020.07.190_bb0270","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.toxicon.2009.09.019","article-title":"A protective immune response against lethal, dermonecrotic and hemorrhagic effects of Loxosceles intermedia venom elicited by a 27-residue peptide","volume":"55","author":"Dias-Lopes","year":"2010","journal-title":"Toxicon"}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813020339453?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813020339453?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:57:01Z","timestamp":1788296221000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141813020339453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":54,"alternative-id":["S0141813020339453"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2020.07.190","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular cloning and functional characterization of recombinant Loxtox from Loxosceles similis venom","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2020.07.190","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}