{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T05:53:13Z","timestamp":1792389193669,"version":"4.4.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T00:00:00Z","timestamp":1674604800000},"content-version":"vor","delay-in-days":330,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1016\/j.ijbiomac.2022.01.107","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T10:56:47Z","timestamp":1642762607000},"page":"597-607","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"special_numbering":"C","title":["NLRP3 inflammasome activation in human peripheral blood mononuclear cells induced by venoms secreted PLA2s"],"prefix":"10.1016","volume":"202","author":[{"given":"Milena Daniela Souza","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica Amaral","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro Valentino","family":"Paloschi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles Nunes","family":"Boeno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Matiele Alves","family":"Rego","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ort\u00eancia","family":"de Oliveira Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hallison Mota","family":"Santana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdison Pereira","family":"dos Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne Nery","family":"Serrath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulamita","family":"da S. Set\u00fabal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson Maciel","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreimar M.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana P.","family":"Zuliani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2020","key":"10.1016\/j.ijbiomac.2022.01.107_bb0005","first-page":"4063562","article-title":"NLRP3 inflammasome and inflammatory diseases","author":"Wang","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0010","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3390\/toxins12010022","article-title":"Inflammasome activation induced by a snake venom Lys49-phospholipase A2 homologue","volume":"12","author":"Boeno","year":"2019","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0015","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1042\/CS20201419","article-title":"Inflammatory effect of Bothropstoxin-I from Bothrops jararacussu venom mediated by NLRP3 inflammasome involves ATP and P2X7 receptor","volume":"135","author":"Silva","year":"2021","journal-title":"Clin. Sci. (Lond)"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0020","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1016\/0041-0101(94)90414-6","article-title":"Edematogenic responses induced by Bothrops jararaca venom in rats: role of lymphocytes","volume":"32","author":"Cury","year":"1994","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0025","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1002\/mus.10453","article-title":"Neutrophils do not contribute to local tissue damage, but play a key role in skeletal muscle regeneration, in mice injected with Bothrops asper snake venom","volume":"28","author":"Teixeira","year":"2003","journal-title":"Muscle Nerve"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0030","series-title":"Inflamm. - From Mol. Cell. Mech. to Clin","first-page":"99","article-title":"Venoms","author":"Teixeira","year":"2017"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0035","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/0006-291X(91)90515-9","article-title":"Interleukin-1\u03b2, tumor necrosis factor and forskolin stimulate the synthesis and secretion of group II phospholipase A2 in rat mesangial cells","volume":"174","author":"Schalkwijk","year":"1991","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0040","doi-asserted-by":"crossref","first-page":"29799","DOI":"10.1074\/jbc.M211763200","article-title":"Potentiation of tumor necrosis factor \u03b1-induced secreted phospholipase A2 (sPLA2)-IIA expression in mesangial cells by an autocrine loop involving sPLA2 and peroxisome proliferator-activated receptor \u03b1 activation","volume":"278","author":"Beck","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0045","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/0041-0101(88)90244-9","article-title":"Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin","volume":"26","author":"Homsi-Brandeburgo","year":"1988","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0050","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0014-2999(97)01546-X","article-title":"Mast cell degranulation induced by two phospholipase A2 homologues: dissociation between enzymatic and biological activities","volume":"343","author":"Landucci","year":"1998","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0055","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1006\/abbi.2000.1790","article-title":"Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A2 homologue from Bothrops neuwiedi pauloensis venom","volume":"378","author":"Soares","year":"2000","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0060","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/S0300-9084(00)01150-0","article-title":"Myotoxic phospholipases A(2) in bothrops snake venoms: effect of chemical modifications on the enzymatic and pharmacological properties of bothropstoxins from Bothrops jararacussu","volume":"82","author":"Andri\u00e3o-Escarso","year":"2000","journal-title":"Biochimie"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0065","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/j.toxicon.2003.11.006","article-title":"Inflammatory effects of snake venom myotoxic phospholipases A2","volume":"42","author":"Teixeira","year":"2003","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0070","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s10565-008-9106-6","article-title":"Toxicity of phospholipases A2 D49 (6\u20131 and 6\u20132) and K49 (Bj-VII) from Bothrops jararacussu venom","volume":"25","author":"Bonfim","year":"2009","journal-title":"Cell Biol. Toxicol."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0075","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1016\/0041-0101(90)90094-N","article-title":"Differences in distribution of myotoxic proteins in venoms from different bothrops species","volume":"28","author":"Moura-da-Silva","year":"1990","journal-title":"Toxicon"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0080","doi-asserted-by":"crossref","DOI":"10.4269\/ajtmh.20-1065","article-title":"Circulating secretory phospholipase A2 activity following snakebites and its relationship with envenomation status and progression of local swelling","author":"Bhardwaj","year":"2020","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0085","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1016\/S0022-2275(20)32226-4","article-title":"A simple assay for a human serum phospholipase A2 that is associated with high-density lipoproteins","volume":"42","author":"Petrovic","year":"2001","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0090","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.tiv.2017.02.003","article-title":"Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells","volume":"41","author":"Pires","year":"2017","journal-title":"Toxicol. in Vitro"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0095","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative CT method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0100","doi-asserted-by":"crossref","first-page":"7271","DOI":"10.1021\/bi900660f","article-title":"N-tosyl-L-phenylalanine chloromethyl ketone inhibits NF-kappaB activation by blocking specific cysteine residues of IkappaB kinase beta and p65\/RelA","volume":"48","author":"Ha","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0105","doi-asserted-by":"crossref","DOI":"10.1038\/cddis.2016.206","article-title":"TLR and NLRP3 inflammasome-dependent innate immune responses to tumor-derived autophagosomes (DRibbles)","volume":"7","author":"Xing","year":"2016","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0110","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1038\/s41589-019-0278-6","article-title":"MCC950 closes the active conformation of NLRP3 to an inactive state","volume":"15","author":"Tapia-Abell\u00e1n","year":"2019","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0115","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nm.3806","article-title":"A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases","volume":"21","author":"Coll","year":"2015","journal-title":"Nat. Med."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0120","doi-asserted-by":"crossref","first-page":"3720","DOI":"10.1182\/blood-2010-07-273417","article-title":"Interleukin-1 in the pathogenesis and treatment of inflammatory diseases","volume":"117","author":"Dinarello","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0125","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/978-1-62703-523-1_7","article-title":"Detection of pyroptosis by measuring released lactate dehydrogenase activity","volume":"1040","author":"Rayamajhi","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0130","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1038\/s41419-019-1413-8","article-title":"Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors","volume":"10","author":"Yang","year":"2019","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0135","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1388-1981(00)00105-0","article-title":"The expanding superfamily of phospholipase A(2) enzymes: classification and characterization","volume":"1488","author":"Six","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0140","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.etp.2005.09.004","article-title":"Paralyzing and myotoxic effects of a recombinant bothropstoxin-I (BthTX-I) on mouse neuromuscular preparations","volume":"57","author":"Gallacci","year":"2006","journal-title":"Exp. Toxicol. Pathol."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0145","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s40409-017-0099-6","article-title":"Structural determinants of the hyperalgesic activity of myotoxic Lys49-phospholipase A2","volume":"23","author":"Zambelli","year":"2017","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0150","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1042\/bj3620089","article-title":"Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis","volume":"362","author":"Ward","year":"2002","journal-title":"Biochem. J."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0155","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1006\/prep.2000.1353","article-title":"Refolding and purification of bothropstoxin-I, a Lys49\u2013Phospholipase A2 homologue, expressed as inclusion bodies in Escherichia coli","volume":"21","author":"Ward","year":"2001","journal-title":"Protein Expr. Purif."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0160","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/BF01024915","article-title":"Bothropstoxin-I: amino acid sequence and function","volume":"12","author":"Cintra","year":"1993","journal-title":"J. Protein Chem."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0165","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0006-2952(01)00841-3","article-title":"Human neutrophil migration in vitro induced by secretory phospholipases A2: a role for cell surface glycosaminoglycans","volume":"63","author":"Gambero","year":"2002","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0175","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.1182\/blood-2008-03-146720","article-title":"Differential requirement for the activation of the inflammasome for processing and release of IL-1\u03b2 in monocytes and macrophages","volume":"113","author":"Netea","year":"2009","journal-title":"Blood"},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0180","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/cr.2011.1","article-title":"A special issue on NF-\u03baB signaling and function","volume":"21","author":"Sun","year":"2011","journal-title":"Cell Res."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0185","doi-asserted-by":"crossref","first-page":"17023","DOI":"10.1038\/sigtrans.2017.23","article-title":"NF-\u03baB signaling in inflammation","volume":"2","author":"Liu","year":"2017","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.ijbiomac.2022.01.107_bb0190","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1002\/eji.201747404","article-title":"The gasdermin-D pore acts as a conduit for IL-1\u03b2 secretion in mice","volume":"48","author":"Heilig","year":"2018","journal-title":"Eur. J. Immunol."}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813022001222?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813022001222?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T09:25:45Z","timestamp":1758878745000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141813022001222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":37,"alternative-id":["S0141813022001222"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2022.01.107","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"NLRP3 inflammasome activation in human peripheral blood mononuclear cells induced by venoms secreted PLA2s","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2022.01.107","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V.","name":"copyright","label":"Copyright"}]}}