{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T22:31:35Z","timestamp":1786487495078,"version":"build-2736575974"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100008363","name":"Sichuan Agricultural University","doi-asserted-by":"publisher","award":["NCTIP-MY23006"],"award-info":[{"award-number":["NCTIP-MY23006"]}],"id":[{"id":"10.13039\/501100008363","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Provincial Science and Technology Support Program","doi-asserted-by":"publisher","award":["2023YFN0021"],"award-info":[{"award-number":["2023YFN0021"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2024,10]]},"DOI":"10.1016\/j.ijbiomac.2024.134151","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T14:51:21Z","timestamp":1721832681000},"page":"134151","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"special_numbering":"P1","title":["Rapid, sensitive, and visual detection of swine Japanese encephalitis virus with a one-pot RPA-CRISPR\/EsCas13d-based dual readout portable platform"],"prefix":"10.1016","volume":"277","author":[{"given":"Dong","family":"You","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing-Zhou","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Shuang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe-Yan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Qi","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Meng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Peng","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuo-Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Qiao","family":"Lang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Cheng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di-Shi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Yuan","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Ru","family":"Ai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Bo","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Wen","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijbiomac.2024.134151_bb0005","doi-asserted-by":"crossref","unstructured":"G.L. Campbell, S.L. Hills, M. Fischer, J.A. Jacobson, C.H. Hoke, J.M. Hombach, A.A. Marfin, T. Solomon, T.F. Tsai, V.D. Tsu, A.S. Ginsburg, Estimated global incidence of Japanese encephalitis: a systematic review, Bull. World Health Organ. 89(10) (2011) 766-74, 774A-774E.","DOI":"10.2471\/BLT.10.085233"},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0010","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/s41598-022-27236-1","article-title":"Worldwide transmission and infection risk of mosquito vectors of West Nile, St. Louis encephalitis, Usutu and Japanese encephalitis viruses: a systematic review","volume":"13","author":"Tols\u00e1-Garc\u00eda","year":"2023","journal-title":"Sci. Rep."},{"issue":"6","key":"10.1016\/j.ijbiomac.2024.134151_bb0015","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.pathol.2022.07.001","article-title":"Emergence of Japanese encephalitis in Australia: a diagnostic perspective","volume":"54","author":"Pham","year":"2023","journal-title":"Pathology"},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0020","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1186\/s13071-019-3501-0","article-title":"Serological evidence of continued Japanese encephalitis virus transmission in Singapore nearly three decades after end of pig farming","volume":"12","author":"Yap","year":"2019","journal-title":"Parasit. Vectors"},{"issue":"10","key":"10.1016\/j.ijbiomac.2024.134151_bb0025","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009385","article-title":"The current burden of Japanese encephalitis and the estimated impacts of vaccination: combining estimates of the spatial distribution and transmission intensity of a zoonotic pathogen","volume":"15","author":"Moore","year":"2021","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0030","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1080\/21505594.2021.1899674","article-title":"Pathogenicity and virulence of Japanese encephalitis virus: neuroinflammation and neuronal cell damage","volume":"12","author":"Ashraf","year":"2021","journal-title":"Virulence"},{"issue":"9","key":"10.1016\/j.ijbiomac.2024.134151_bb0035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0000437","article-title":"Japanese encephalitis-a pathological and clinical perspective","volume":"3","author":"Ghosh","year":"2009","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"6","key":"10.1016\/j.ijbiomac.2024.134151_bb0040","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.pathol.2022.07.001","article-title":"Emergence of Japanese encephalitis in Australia: a diagnostic perspective","volume":"54","author":"Pham","year":"2022","journal-title":"Pathology"},{"issue":"3","key":"10.1016\/j.ijbiomac.2024.134151_bb0045","first-page":"370","article-title":"Japanese encephalitis can be devastating","volume":"36","author":"Lopalco","year":"2024","journal-title":"Ann. Ig."},{"issue":"9","key":"10.1016\/j.ijbiomac.2024.134151_bb0050","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.4014\/jmb.2207.07068","article-title":"Prevalence and genetic characteristics of Japanese encephalitis virus among mosquitoes and pigs in Hunan Province, China from 2019 to 2021","volume":"32","author":"Tang","year":"2022","journal-title":"J. Microbiol. Biotechnol."},{"issue":"3","key":"10.1016\/j.ijbiomac.2024.134151_bb0055","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0194412","article-title":"Development of an improved RT-qPCR assay for detection of Japanese encephalitis virus (JEV) RNA including a systematic review and comprehensive comparison with published methods","volume":"13","author":"Bharucha","year":"2018","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.ijbiomac.2024.134151_bb0060","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.jcv.2005.11.004","article-title":"Development and evaluation of a rapid IgM capture ELISA (JEV-Chex) for the diagnosis of Japanese encephalitis","volume":"35","author":"Ravi","year":"2006","journal-title":"J. Clin. Virol."},{"issue":"2","key":"10.1016\/j.ijbiomac.2024.134151_bb0065","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.molcel.2014.03.011","article-title":"CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity","volume":"54","author":"Barrangou","year":"2014","journal-title":"Mol. Cell"},{"issue":"5","key":"10.1016\/j.ijbiomac.2024.134151_bb0070","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.cell.2016.04.059","article-title":"Rapid, low-cost detection of Zika virus using programmable biomolecular components","volume":"165","author":"Pardee","year":"2016","journal-title":"Cell"},{"issue":"7","key":"10.1016\/j.ijbiomac.2024.134151_bb0075","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.00261-22","article-title":"COVID-19 variant detection with a high-fidelity CRISPR-Cas12 enzyme","volume":"60","author":"Fasching","year":"2022","journal-title":"J. Clin. Microbiol."},{"issue":"10","key":"10.1016\/j.ijbiomac.2024.134151_bb0080","article-title":"CRISPR-enhanced hydrogel microparticles for multiplexed detection of nucleic acids","volume":"10","author":"Roh","year":"2023","journal-title":"Adv. Sci. (Weinh)"},{"issue":"6387","key":"10.1016\/j.ijbiomac.2024.134151_bb0085","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0090","doi-asserted-by":"crossref","first-page":"1060947","DOI":"10.3389\/fmicb.2022.1060947","article-title":"CRISPR-Cas13a system: a novel tool for molecular diagnostics","volume":"13","author":"Zhao","year":"2022","journal-title":"Front. Microbiol."},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0095","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1186\/s13059-021-02495-9","article-title":"PAM-repeat associations and spacer selection preferences in single and co-occurring CRISPR-Cas systems","volume":"22","author":"Vink","year":"2021","journal-title":"Genome Biol."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0100","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.866800","article-title":"Cas13d: a new molecular scissor for transcriptome engineering","volume":"10","author":"Gupta","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0105","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.cell.2018.09.001","article-title":"Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d","volume":"175","author":"Zhang","year":"2018","journal-title":"Cell"},{"issue":"18","key":"10.1016\/j.ijbiomac.2024.134151_bb0110","doi-asserted-by":"crossref","first-page":"5739","DOI":"10.1007\/s00253-023-12690-2","article-title":"Development of an RT-PCR-based RspCas13d system to detect porcine deltacoronavirus","volume":"107","author":"Zhao","year":"2023","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0115","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.976137","article-title":"Visual detection and differentiation of porcine epidemic diarrhea virus wild-type strains and attenuated vaccine strains using CRISPR\/Cas13a -based lateral flow strip","volume":"12","author":"Yin","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0120","doi-asserted-by":"crossref","first-page":"1273988","DOI":"10.3389\/fbioe.2023.1273988","article-title":"Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection","volume":"11","author":"Mao","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0125","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2023.104730","article-title":"Development of CRISPR\/Cas13a-based assays for the diagnosis of schistosomiasis","volume":"94","author":"MacGregor","year":"2023","journal-title":"EBioMedicine"},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2023.125413","article-title":"Establishment of an ultrasensitive and visual detection platform for Neospora caninum based-on the RPA-CRISPR\/Cas12a system","volume":"269","author":"Wang","year":"2024","journal-title":"Talanta"},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114819","article-title":"Hybrid CRISPR\/Cas protein for one-pot detection of DNA and RNA","volume":"219","author":"Guk","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0140","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2023.341059","article-title":"One-pot RPA-Cas12a assay for instant and visual detection of Burkholderia pseudomallei","volume":"1252","author":"Deng","year":"2023","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.113994","article-title":"A one-pot CRISPR\/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics","volume":"202","author":"Hu","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0150","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2022.132767","article-title":"Increasing trans-cleavage catalytic efficiency of Cas12a and Cas13a with chemical enhancers: application to amplified nucleic acid detection","volume":"373","author":"Deng","year":"2022","journal-title":"Sensors Actuators B Chem."},{"issue":"8","key":"10.1016\/j.ijbiomac.2024.134151_bb0155","doi-asserted-by":"crossref","first-page":"3037","DOI":"10.1002\/bit.27813","article-title":"Sensitive analysis of single nucleotide variation by Cas13d orthologs, EsCas13d and RspCas13d","volume":"118","author":"Qiao","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0160","article-title":"Rapid differential detection of Japanese encephalitis virus and Getah virus in pigs or mosquitos by a duplex TaqMan real-time RT-PCR assay","volume":"9","author":"Zhang","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0165","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.125967","article-title":"Rapid, sensitive, and visual detection of Clonorchis sinensis with an RPA-CRISPR\/Cas12a-based dual readout portable platform","volume":"249","author":"Huang","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0170","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3201\/eid2601.190235","article-title":"Distribution of Japanese encephalitis virus, Japan and Southeast Asia, 2016-2018","volume":"26","author":"Kuwata","year":"2020","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0175","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/21645515.2017.1380756","article-title":"Risk assessment for Japanese encephalitis vaccination","volume":"14","author":"Turtle","year":"2018","journal-title":"Hum. Vaccin. Immunother."},{"issue":"3","key":"10.1016\/j.ijbiomac.2024.134151_bb0180","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1007\/s11481-013-9455-7","article-title":"Japanese encephalitis virus infection alters both neuronal and astrocytic differentiation of neural stem\/progenitor cells","volume":"8","author":"Ariff","year":"2013","journal-title":"J. NeuroImmune Pharmacol."},{"issue":"11","key":"10.1016\/j.ijbiomac.2024.134151_bb0185","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.3390\/vaccines11111683","article-title":"Japanese encephalitis vaccine acceptance and strategies for travelers: insights from a scoping review and practitioners in endemic countries","volume":"11","author":"Asawapaithulsert","year":"2023","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0190","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2022.198869","article-title":"A one-tube rapid visual CRISPR assay for the field detection of Japanese encephalitis virus","volume":"319","author":"Xu","year":"2022","journal-title":"Virus Res."},{"issue":"8","key":"10.1016\/j.ijbiomac.2024.134151_bb0195","doi-asserted-by":"crossref","first-page":"769","DOI":"10.3390\/bios13080769","article-title":"A CRISPR-Cas12a-based diagnostic method for Japanese encephalitis virus genotypes I, III, and V","volume":"13","author":"Kwak","year":"2023","journal-title":"Biosensors (Basel)"},{"issue":"1","key":"10.1016\/j.ijbiomac.2024.134151_bb0200","doi-asserted-by":"crossref","first-page":"9392","DOI":"10.1038\/s41598-022-13604-4","article-title":"Epidemiological investigation of swine Japanese encephalitis virus based on RT-RAA detection method","volume":"12","author":"Nie","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijbiomac.2024.134151_bb0205","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113701","article-title":"Exploiting the orthogonal CRISPR-Cas12a\/Cas13a trans-cleavage for dual -gene virus detection using a handheld device","volume":"196","author":"Tian","year":"2022","journal-title":"Biosens. Bioelectron."},{"issue":"2","key":"10.1016\/j.ijbiomac.2024.134151_bb0210","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.cell.2020.12.001","article-title":"Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy","volume":"184","author":"Fozouni","year":"2021","journal-title":"Cell"},{"issue":"7811","key":"10.1016\/j.ijbiomac.2024.134151_bb0215","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/s41586-020-2279-8","article-title":"Massively multiplexed nucleic acid detection with Cas13","volume":"582","author":"Ackerman","year":"2020","journal-title":"Nature"}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813024049560?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813024049560?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T22:15:40Z","timestamp":1786486540000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141813024049560"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":43,"alternative-id":["S0141813024049560"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2024.134151","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid, sensitive, and visual detection of swine Japanese encephalitis virus with a one-pot RPA-CRISPR\/EsCas13d-based dual readout portable platform","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2024.134151","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"134151"}}