{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T05:16:45Z","timestamp":1770095805268,"version":"3.49.0"},"reference-count":75,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001849","name":"Defence Research and Development Organisation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001849","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.ijbiomac.2026.150396","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T16:37:26Z","timestamp":1769013446000},"page":"150396","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"P1","title":["Design and development of a novel recombinant multi-epitope antigen: A promising candidate for melioidosis disease diagnosis"],"prefix":"10.1016","volume":"344","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4398-9838","authenticated-orcid":false,"given":"Pranjal Kumar","family":"Yadav","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3777-0707","authenticated-orcid":false,"given":"Jyotsna","family":"Dhubkarya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4122-6129","authenticated-orcid":false,"given":"Suchetna","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2752-4340","authenticated-orcid":false,"given":"Moumita","family":"Paul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4233-3506","authenticated-orcid":false,"given":"Sanjay","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9813-7373","authenticated-orcid":false,"given":"Ponmariappan","family":"Sarkaraisamy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0046-9426","authenticated-orcid":false,"given":"Duraipandian","family":"Thavaselvam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.ijbiomac.2026.150396_bb0005","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","article-title":"Melioidosis: epidemiology, pathophysiology, and management","volume":"18","author":"Cheng","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"issue":"12","key":"10.1016\/j.ijbiomac.2026.150396_bb0010","doi-asserted-by":"crossref","first-page":"E17","DOI":"10.2807\/esm.09.12.00507-en","article-title":"Task force on biological and chemical agent threat, public health directorate, European Commission, Luxembourg, Bichat guidelines for the clinical management of glanders and melioidosis and bioterrorism-related glanders and melioidosis","volume":"9","author":"Bossi","year":"2004","journal-title":"Euro Surveill."},{"issue":"4","key":"10.1016\/j.ijbiomac.2026.150396_bb0015","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/nrmicro1385","article-title":"Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei","volume":"4","author":"Wiersinga","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"issue":"2","key":"10.1016\/j.ijbiomac.2026.150396_bb0020","doi-asserted-by":"crossref","first-page":"149","DOI":"10.3390\/pathogens11020149","article-title":"The epidemiology of melioidosis and its association with diabetes mellitus: a systematic review and meta-analysis","volume":"11","author":"Chowdhury","year":"2022","journal-title":"Pathogens"},{"issue":"7","key":"10.1016\/j.ijbiomac.2026.150396_bb0025","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1007\/s00284-024-03748-z","article-title":"Diabetes and infectious diseases with a focus on melioidosis","volume":"81","author":"Uthaya Kumar","year":"2024","journal-title":"Curr. Microbiol."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0030","doi-asserted-by":"crossref","DOI":"10.1038\/nmicrobiol.2015.8","article-title":"Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis","volume":"1","author":"Limmathurotsakul","year":"2016","journal-title":"Nat. Microbiol."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0035","doi-asserted-by":"crossref","DOI":"10.1038\/nrdp.2017.107","article-title":"Melioidosis","author":"Wiersinga","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"issue":"6","key":"10.1016\/j.ijbiomac.2026.150396_bb0040","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.3201\/eid2806.211526","article-title":"Burkholderia pseudomallei in environment of adolescent siblings with melioidosis, Kerala, India","volume":"28","author":"Bhaskaran","year":"2022","journal-title":"Emerg. Infect. Dis."},{"issue":"9","key":"10.1016\/j.ijbiomac.2026.150396_bb0045","doi-asserted-by":"crossref","first-page":"4981","DOI":"10.4103\/jfmpc.jfmpc_1_22","article-title":"Prevention of melioidosis","volume":"11","author":"Mohapatra","year":"2022","journal-title":"J. Family Med. Prim. Care"},{"issue":"2","key":"10.1016\/j.ijbiomac.2026.150396_bb0050","doi-asserted-by":"crossref","first-page":"e00006","DOI":"10.1128\/CMR.00006-19","article-title":"Human Melioidosis","volume":"33","author":"Gassiep","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"issue":"2\u20133","key":"10.1016\/j.ijbiomac.2026.150396_bb0055","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0001-706X(99)00060-1","article-title":"The epidemiology of melioidosis in Australia and Papua New Guinea","volume":"74","author":"Currie","year":"2000","journal-title":"Acta Trop."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0060","doi-asserted-by":"crossref","DOI":"10.1136\/bcr-2017-221787","article-title":"Melioidosis mimicking tubercular cold abscess","volume":"2017","author":"Kundangar","year":"2017","journal-title":"BMJ Case Rep."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0065","series-title":"The Sunday Times, Sri Lanka, Melioidosis: A Deadly Bug Hiding Under Another Identity","author":"Hettiarachchi","year":"2016"},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0070","doi-asserted-by":"crossref","first-page":"84.e1","DOI":"10.1016\/j.cmi.2017.07.029","article-title":"A simple laboratory algorithm for diagnosis of melioidosis in resource-constrained areas: a study from north-Central Vietnam","volume":"24","author":"Trinh","year":"2018","journal-title":"Clin. Microbiol. Infect."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0075","doi-asserted-by":"crossref","DOI":"10.1080\/21505594.2025.2505698","article-title":"Melioidosis molecular diagnostics: an update","volume":"16","author":"Gangil","year":"2025","journal-title":"Virulence"},{"issue":"8","key":"10.1016\/j.ijbiomac.2026.150396_bb0080","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0012485","article-title":"Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models","volume":"5","author":"Limmathurotsakul","year":"2010","journal-title":"PLoS One"},{"issue":"2","key":"10.1016\/j.ijbiomac.2026.150396_bb0085","article-title":"Melioidosis diagnostic workshop, 2013","volume":"21","author":"Hoffmaster","year":"2015","journal-title":"Emerg. Infect. Dis."},{"issue":"Pt 10","key":"10.1016\/j.ijbiomac.2026.150396_bb0090","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1099\/jmm.0.041525-0","article-title":"Misidentification of Burkholderia pseudomallei as Burkholderia cepacia by the VITEK 2 system","volume":"61","author":"Zong","year":"2012","journal-title":"J. Med. Microbiol."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0095","doi-asserted-by":"crossref","first-page":"7","DOI":"10.4103\/jgid.jgid_39_17","article-title":"Influence of biochemical features of Burkholderia pseudomallei strains on identification reliability by Vitek 2 system","volume":"10","author":"Zakharova","year":"2018","journal-title":"J. Global Infect. Dis."},{"issue":"Pt 1","key":"10.1016\/j.ijbiomac.2026.150396_bb0100","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1099\/jmm.0.080317-0","article-title":"Performance of the VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid bacterial identification in two diagnostic centres in China","volume":"64","author":"Luo","year":"2015","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0105","first-page":"415","article-title":"Evaluation of the Bruker Biotyper matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry system for identification of clinical and environmental isolates of Burkholderia pseudomallei","volume":"7","author":"Wang","year":"2016","journal-title":"Front. Microbiol."},{"issue":"8","key":"10.1016\/j.ijbiomac.2026.150396_bb0110","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1099\/jmm.0.000098","article-title":"Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay","volume":"64","author":"Robertson","year":"2015","journal-title":"J. Med. Microbiol."},{"issue":"7","key":"10.1016\/j.ijbiomac.2026.150396_bb0115","doi-asserted-by":"crossref","first-page":"e02002","DOI":"10.1128\/JCM.02002-17","article-title":"Evaluation of a rapid diagnostic test for detection of Burkholderia pseudomallei in the Lao People\u2019s Democratic Republic","volume":"56","author":"Woods","year":"2018","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.ijbiomac.2026.150396_bb0120","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0194595","article-title":"Performance evaluation of active Melioidosis detect-lateral flow assay (AMD-LFA) for diagnosis of melioidosis in endemic settings with limited resources","volume":"13","author":"Shaw","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0125","first-page":"23","article-title":"PCR-based methodologies used to detect and differentiate the Burkholderia pseudomallei complex: B. pseudomallei, B. mallei, and B. thailandensis","volume":"16","author":"Lowe","year":"2014","journal-title":"Curr Issues Mol Biol."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0130","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.mimet.2011.10.023","article-title":"Towards a rapid molecular diagnostic for melioidosis: comparison of DNA extraction methods from clinical specimens","volume":"88","author":"Richardson","year":"2012","journal-title":"J. Microbiol. Methods"},{"issue":"5","key":"10.1016\/j.ijbiomac.2026.150396_bb0135","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1590\/S0036-46652006000500001","article-title":"PCR-based identification of Burkholderia pseudomallei","volume":"48","author":"Merritt","year":"2006","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"issue":"5","key":"10.1016\/j.ijbiomac.2026.150396_bb0140","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0037723","article-title":"Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications","volume":"7","author":"Price","year":"2012","journal-title":"PLoS One"},{"issue":"5","key":"10.1016\/j.ijbiomac.2026.150396_bb0145","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1128\/JCM.02856-15","article-title":"Development of rapid enzyme-linked immunosorbent assays for detection of antibodies to Burkholderia pseudomallei","volume":"54","author":"Suttisunhakul","year":"2016","journal-title":"J. Clin. Microbiol."},{"issue":"6","key":"10.1016\/j.ijbiomac.2026.150396_bb0150","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.4269\/ajtmh.2006.74.1074","article-title":"Development of antibodies to Burkholderia pseudomallei during childhood in melioidosis-endemic Northeast Thailand","volume":"74","author":"Wuthiekanun","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"2","key":"10.1016\/j.ijbiomac.2026.150396_bb0155","doi-asserted-by":"crossref","first-page":"330","DOI":"10.4269\/ajtmh.2006.74.330","article-title":"Indirect hemagglutination assay in patients with melioidosis in northern Australia","volume":"74","author":"Cheng","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"6","key":"10.1016\/j.ijbiomac.2026.150396_bb0160","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.4269\/ajtmh.17-0998","article-title":"Antibodies in Melioidosis: the role of the indirect Hemagglutination assay in evaluating patients and exposed populations","volume":"99","author":"Chaichana","year":"2018","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"7","key":"10.1016\/j.ijbiomac.2026.150396_bb0165","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004847","article-title":"Rapid and sensitive multiplex detection of Burkholderia pseudomallei-specific antibodies in Melioidosis patients based on a protein microarray approach","volume":"10","author":"Kohler","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"8","key":"10.1016\/j.ijbiomac.2026.150396_bb0170","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00346-18","article-title":"A rapid Immunochromatography test based on Hcp1 is a potential point-of-care test for serological diagnosis of Melioidosis","volume":"56","author":"Phokrai","year":"2018","journal-title":"J. Clin. Microbiol."},{"issue":"7","key":"10.1016\/j.ijbiomac.2026.150396_bb0175","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0008452","article-title":"Melioidosis DS rapid test: a standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection","volume":"14","author":"Wagner","year":"2020","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2020.122002","article-title":"Point-of-care cancer diagnostic devices: from academic research to clinical translation","volume":"225","author":"Syedmoradi","year":"2021","journal-title":"Talanta"},{"issue":"3","key":"10.1016\/j.ijbiomac.2026.150396_bb0185","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1128\/CVI.05553-11","article-title":"Evaluation of a recombinant multiepitope peptide for serodiagnosis of toxoplasma gondii infection","volume":"19","author":"Dai","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0190","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s13071-016-1418-4","article-title":"Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay","volume":"9","author":"Lv","year":"2016","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0195","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1007\/s10989-019-09940-1","article-title":"Designing and modeling of multi-epitope proteins for diagnosis of Toxocara canis infection","volume":"26","author":"Ebrahimi","year":"2020","journal-title":"Int. J. Pept. Res. Ther."},{"issue":"32","key":"10.1016\/j.ijbiomac.2026.150396_bb0200","doi-asserted-by":"crossref","first-page":"13499","DOI":"10.1073\/pnas.0812080106","article-title":"A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens","volume":"106","author":"Felgner","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"14","key":"10.1016\/j.ijbiomac.2026.150396_bb0205","doi-asserted-by":"crossref","first-page":"5203","DOI":"10.3390\/ijerph17145203","article-title":"Distribution of serological response to Burkholderia pseudomallei in swine from three provinces of Vietnam","volume":"17","author":"Norris","year":"2020","journal-title":"Int. J. Environ. Res. Public Health"},{"issue":"13","key":"10.1016\/j.ijbiomac.2026.150396_bb0210","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1093\/bioinformatics\/btq249","article-title":"PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes","volume":"26","author":"Yu","year":"2010","journal-title":"Bioinformatics"},{"issue":"3","key":"10.1016\/j.ijbiomac.2026.150396_bb0215","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"issue":"4","key":"10.1016\/j.ijbiomac.2026.150396_bb0220","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/s41587-019-0036-z","article-title":"SignalP 5.0 improves signal peptide predictions using deep neural networks","volume":"37","author":"Almagro Armenteros","year":"2019","journal-title":"Nat. Biotechnol."},{"issue":"23","key":"10.1016\/j.ijbiomac.2026.150396_bb0225","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1093\/bioinformatics\/btq551","article-title":"High-throughput prediction of protein antigenicity using protein microarray data","volume":"26","author":"Magnan","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0230","doi-asserted-by":"crossref","first-page":"298","DOI":"10.3389\/fimmu.2019.00298","article-title":"Antibody specific B-cell epitope predictions: leveraging information from antibody-antigen protein complexes","volume":"10","author":"Jespersen","year":"2019","journal-title":"Front. Immunol."},{"issue":"9","key":"10.1016\/j.ijbiomac.2026.150396_bb0235","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0045152","article-title":"SVMTriP: a method to predict antigenic epitopes using support vector machine to integrate tri-peptide similarity and propensity","volume":"7","author":"Yao","year":"2012","journal-title":"PLoS One"},{"issue":"W1","key":"10.1016\/j.ijbiomac.2026.150396_bb0240","doi-asserted-by":"crossref","first-page":"W24","DOI":"10.1093\/nar\/gkx346","article-title":"BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes","volume":"45","author":"Jespersen","year":"2017","journal-title":"Nucleic Acids Res."},{"issue":"4","key":"10.1016\/j.ijbiomac.2026.150396_bb0245","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/jmr.893","article-title":"Predicting linear B-cell epitopes using string kernels","volume":"21","author":"El-Manzalawy","year":"2008","journal-title":"J. Mol. Recognit."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0250","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1186\/1471-2105-9-514","article-title":"ElliPro: a new structure-based tool for the prediction of antibody epitopes","volume":"9","author":"Ponomarenko","year":"2008","journal-title":"BMC Bioinformatics"},{"issue":"11","key":"10.1016\/j.ijbiomac.2026.150396_bb0255","doi-asserted-by":"crossref","first-page":"5929","DOI":"10.1073\/pnas.95.11.5929","article-title":"Optimizing the stability of single-chain proteins by linker length and composition mutagenesis","volume":"95","author":"Robinson","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"42","key":"10.1016\/j.ijbiomac.2026.150396_bb0260","doi-asserted-by":"crossref","first-page":"34979","DOI":"10.1074\/jbc.M112.374504","article-title":"The methionine-aromatic motif plays a unique role in stabilizing protein structure","volume":"287","author":"Valley","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0265","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.mie.2014.11.003","article-title":"Purification of his-tagged proteins","volume":"559","author":"Spriestersbach","year":"2015","journal-title":"Methods Enzymol."},{"issue":"6","key":"10.1016\/j.ijbiomac.2026.150396_bb0270","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"EMBOSS: the European molecular biology open software suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0275","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gene.2016.02.047","article-title":"Synonymous codon usage pattern in glycoprotein gene of rabies virus","volume":"584","author":"Morla","year":"2016","journal-title":"Gene"},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0280","first-page":"531","article-title":"Protein identification and analysis tools in the ExPASy server","volume":"112","author":"Wilkins","year":"1999","journal-title":"Methods Mol. Biol."},{"issue":"4","key":"10.1016\/j.ijbiomac.2026.150396_bb0285","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1093\/bioinformatics\/16.4.404","article-title":"The PSIPRED protein structure prediction server","volume":"16","author":"McGuffin","year":"2000","journal-title":"Bioinformatics"},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0290","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1005324","article-title":"Accurate de novo prediction of protein contact map by ultra-deep learning model","volume":"13","author":"Wang","year":"2017","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0295","first-page":"253","article-title":"Transformation of plasmid DNA into E. coli using the heat shock method","volume":"6","author":"Froger","year":"2007","journal-title":"J. Vis. Exp."},{"issue":"3","key":"10.1016\/j.ijbiomac.2026.150396_bb0300","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1006\/jmbi.1996.0399","article-title":"Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels","volume":"260","author":"Miroux","year":"1996","journal-title":"J. Mol. Biol."},{"issue":"5259","key":"10.1016\/j.ijbiomac.2026.150396_bb0305","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0310","article-title":"Falke, reprint of: purification of proteins using polyhistidine affinity tags","volume":"3","author":"Bornhorst","year":"2011","journal-title":"Protein Expr. Purif."},{"issue":"6","key":"10.1016\/j.ijbiomac.2026.150396_bb0315","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.parint.2014.07.004","article-title":"Molecular cloning and biochemical characterization of iron superoxide dismutase from the rodent malaria parasite Plasmodium vinckei","volume":"63","author":"Prakash","year":"2014","journal-title":"Parasitol. Int."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0320","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"issue":"2","key":"10.1016\/j.ijbiomac.2026.150396_bb0325","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/BF02789065","article-title":"Identification of glycoproteins on nitrocellulose membranes and gels","volume":"5","author":"Thornton","year":"1996","journal-title":"Mol. Biotechnol."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0330","doi-asserted-by":"crossref","first-page":"8543","DOI":"10.1038\/s41598-020-65347-9","article-title":"Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis","volume":"10","author":"Hans","year":"2020","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.ijbiomac.2026.150396_bb0335","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.diagmicrobio.2014.10.003","article-title":"Cloning, expression and purification of outer membrane protein (OmpA) of Burkholderia pseudomallei and evaluation of its potential for serodiagnosis of melioidosis","volume":"81","author":"Arora","year":"2015","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ijbiomac.2026.150396_bb0340","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/3039106","article-title":"Burkholderia pseudomallei adaptation for survival in stressful conditions","volume":"2018","author":"Duangurai","year":"2018","journal-title":"Biomed. Res. Int."},{"issue":"9","key":"10.1016\/j.ijbiomac.2026.150396_bb0345","doi-asserted-by":"crossref","first-page":"5945","DOI":"10.1128\/IAI.73.9.5945-5951.2005","article-title":"Humoral and cell-mediated adaptive immune responses are required for protection against Burkholderia pseudomallei challenge and bacterial clearance postinfection","volume":"73","author":"Healey","year":"2005","journal-title":"Infect. Immun."},{"issue":"16","key":"10.1016\/j.ijbiomac.2026.150396_bb0350","doi-asserted-by":"crossref","first-page":"4992","DOI":"10.1128\/JB.185.16.4992-4996.2003","article-title":"A Burkholderia pseudomallei type III secreted protein, BopE, facilitates bacterial invasion of epithelial cells and exhibits guanine nucleotide exchange factor activity","volume":"185","author":"Stevens","year":"2003","journal-title":"J. Bacteriol."},{"issue":"3","key":"10.1016\/j.ijbiomac.2026.150396_bb0355","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1046\/j.1365-2958.2002.03190.x","article-title":"An Inv\/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen","volume":"46","author":"Stevens","year":"2002","journal-title":"Mol. Microbiol."},{"issue":"Pt 8","key":"10.1016\/j.ijbiomac.2026.150396_bb0360","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1099\/mic.0.27146-0","article-title":"Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis","volume":"150","author":"Stevens","year":"2004","journal-title":"Microbiology"},{"issue":"18","key":"10.1016\/j.ijbiomac.2026.150396_bb0365","doi-asserted-by":"crossref","first-page":"6556","DOI":"10.1128\/JB.187.18.6556-6560.2005","article-title":"Multinucleated giant cell formation and apoptosis in infected host cells is mediated by Burkholderia pseudomallei type III secretion protein BipB","volume":"187","author":"Suparak","year":"2005","journal-title":"J. Bacteriol."},{"issue":"4","key":"10.1016\/j.ijbiomac.2026.150396_bb0370","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1128\/IAI.01218-10","article-title":"The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei","volume":"79","author":"Burtnick","year":"2011","journal-title":"Infect. Immun."},{"issue":"1","key":"10.1016\/j.ijbiomac.2026.150396_bb0375","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1574-695X.2007.00345.x","article-title":"Evaluating Burkholderia pseudomallei Bip proteins as vaccines and Bip antibodies as detection agents","volume":"52","author":"Druar","year":"2008","journal-title":"FEMS Immunol. Med. Microbiol."}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813026003223?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0141813026003223?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T17:14:22Z","timestamp":1770052462000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0141813026003223"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":75,"alternative-id":["S0141813026003223"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2026.150396","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Design and development of a novel recombinant multi-epitope antigen: A promising candidate for melioidosis disease diagnosis","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2026.150396","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"150396"}}