{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T18:23:06Z","timestamp":1794594186068,"version":"4.9.1"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-64-00017p"],"award-info":[{"award-number":["21-64-00017p"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biological Macromolecules"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.ijbiomac.2026.154663","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:27:42Z","timestamp":1790688462000},"page":"154663","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"P2","title":["Pre-steady-state kinetic analysis of dNTP and DNA binding by human terminal deoxynucleotidyl transferase"],"prefix":"10.1016","volume":"383","author":[{"given":"Svetlana S.","family":"Senchurova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra A.","family":"Kuznetsova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita A.","family":"Kuznetsov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijbiomac.2026.154663_bb0005","first-page":"1","article-title":"Tdt the story of a misguided DNA polymerase","volume":"23","author":"Motea","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0010","doi-asserted-by":"crossref","first-page":"2780","DOI":"10.1016\/S0021-9258(18)99635-3","article-title":"Deoxynucleotide-polymerizing enzymes of calf thymus gland. II. Properties of the terminal deoxynucleotidyltransferase","volume":"242","author":"Kato","year":"1967","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0092-8674(78)90078-8","article-title":"A complete immunoglobulin gene is created by somatic recombination","volume":"15","author":"Brack","year":"1978","journal-title":"Cell"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0020","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/312036a0","article-title":"A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes","volume":"312","author":"Saito","year":"1984","journal-title":"Nature"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0025","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1093\/nar\/29.7.1399","article-title":"The RAG proteins in V(D)J recombination: more than just a nuclease","volume":"29","author":"Sadofsky","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0030","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nri2941","article-title":"Recombination centres and the orchestration of V(D)J recombination","volume":"11","author":"Schatz","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0035","doi-asserted-by":"crossref","first-page":"6373","DOI":"10.3390\/ijms23126373","article-title":"Structural and molecular kinetic features of activities of DNA polymerases","volume":"23","author":"Kuznetsova","year":"2022","journal-title":"IJMS"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0040","doi-asserted-by":"crossref","first-page":"5905","DOI":"10.2741\/3125","article-title":"phosphopeptide binding and signaling modules","volume":"13","author":"Rodriguez","year":"2008","journal-title":"Front. Biosci."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0045","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1093\/emboj\/21.3.427","article-title":"Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase","volume":"21","author":"Delarue","year":"2002","journal-title":"EMBO J."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0050","doi-asserted-by":"crossref","first-page":"4334","DOI":"10.1016\/j.jmb.2013.07.009","article-title":"Structures of intermediates along the catalytic cycle of terminal deoxynucleotidyltransferase: dynamical aspects of the two-metal ion mechanism","volume":"425","author":"Gouge","year":"2013","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0055","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25020879","article-title":"Substrate specificity diversity of human terminal deoxynucleotidyltransferase may be a naturally programmed feature facilitating its biological function","volume":"25","author":"Kuznetsova","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0060","first-page":"1","article-title":"The role of active site hydrophobic interactions in facilitating catalysis in human terminal deoxynucleotidyl transferase","author":"Senchurova","year":"2026","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0065","doi-asserted-by":"crossref","first-page":"4642","DOI":"10.1093\/nar\/gkp460","article-title":"Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region","volume":"37","author":"Romain","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0070","doi-asserted-by":"crossref","first-page":"11859","DOI":"10.1016\/S0021-9258(17)32652-2","article-title":"Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase","volume":"269","author":"Yang","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0075","doi-asserted-by":"crossref","first-page":"6498","DOI":"10.1073\/pnas.90.14.6498","article-title":"A general two-metal-ion mechanism for catalytic RNA","volume":"90","author":"Steitz","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0080","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1134\/S0006297925602345","article-title":"Coordination of nucleoside triphosphates in the active site of the human TdT during template-independent DNA synthesis","volume":"91","author":"Senchurova","year":"2026","journal-title":"Biochem. Mosc."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0085","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1073\/pnas.69.8.2039","article-title":"A fluorescent analog of nicotinamide adenine dinucleotide","volume":"69","author":"Barrio","year":"1972","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0090","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1073\/pnas.72.10.3966","article-title":"Dynamic and static quenching of 1,N6-ethenoadenine fluorescence in nicotinamide 1,NV6-ethenoadenine dinucleotide and in 1,N6-etheno-9-[3-(indol-3-yl)propyl]adenine","volume":"72","author":"Gruber","year":"1975","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0095","doi-asserted-by":"crossref","first-page":"20189","DOI":"10.1039\/C6CP01559J","article-title":"Photophysical properties of pyrrolocytosine, a cytosine fluorescent base analogue","volume":"18","author":"Nguyen","year":"2016","journal-title":"Phys. Chem. Chem. Phys."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0100","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1002\/cbic.200700096","article-title":"The use of non-natural nucleotides to probe template-independent DNA synthesis","volume":"8","author":"Berdis","year":"2007","journal-title":"ChemBioChem"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0105","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1039\/c0ob00856g","article-title":"Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl transferase. Application in electrochemical DNA hybridization and protein-DNA binding assays","volume":"9","author":"Hor\u00e1kov\u00e1","year":"2011","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0110","doi-asserted-by":"crossref","first-page":"5162","DOI":"10.1039\/c3ob40842f","article-title":"Deoxynucleoside triphosphates bearing histamine, carboxylic acid, and hydroxyl residues-synthesis and biochemical characterization","volume":"11","author":"Hollenstein","year":"2013","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0115","doi-asserted-by":"crossref","first-page":"13031","DOI":"10.1039\/C7CC07207D","article-title":"Facile immobilization of DNA using an enzymatic his-tag mimic","volume":"53","author":"R\u00f6thlisberger","year":"2017","journal-title":"Chem. Commun."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0120","doi-asserted-by":"crossref","first-page":"1","DOI":"10.26508\/lsa.202201428","article-title":"Insight into the mechanism of DNA synthesis by human terminal deoxynucleotidyltransferase","volume":"5","author":"Kuznetsova","year":"2022","journal-title":"Life Sci. Alliance"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0125","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1006\/abio.1996.0238","article-title":"Program DYNAFIT for the analysis of enzyme kinetic data: application to HIV proteinase","volume":"237","author":"Kuzmi\u010d","year":"1996","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0130","series-title":"Computer Methods Part B","first-page":"247","article-title":"Chapter 10 - dynafit\u2014a software package for enzymology","author":"Kuzmi\u010d","year":"2009"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0135","doi-asserted-by":"crossref","first-page":"190","DOI":"10.3390\/genes9040190","article-title":"Kinetics and thermodynamics of DNA processing by wild type DNA-glycosylase endo III and its catalytically inactive mutant forms","volume":"9","author":"Kladova","year":"2018","journal-title":"Genes"},{"key":"10.1016\/j.ijbiomac.2026.154663_bb0140","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1080\/07391102.2009.10507278","article-title":"Conformational dynamics of human AP endonuclease in base excision and nucleotide incision repair pathways","volume":"26","author":"Timofeyeva","year":"2009","journal-title":"J. Biomol. Struct. Dyn."}],"container-title":["International Journal of Biological Macromolecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S014181302604612X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S014181302604612X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T17:48:14Z","timestamp":1794592094000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S014181302604612X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":28,"alternative-id":["S014181302604612X"],"URL":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2026.154663","relation":{},"ISSN":["0141-8130"],"issn-type":[{"value":"0141-8130","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Pre-steady-state kinetic analysis of dNTP and DNA binding by human terminal deoxynucleotidyl transferase","name":"articletitle","label":"Article Title"},{"value":"International Journal of Biological Macromolecules","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijbiomac.2026.154663","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"154663"}}