{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:48:50Z","timestamp":1789591730345,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,1,7]],"date-time":"2007-01-07T00:00:00Z","timestamp":1168128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007042","name":"Third World Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007042","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J of Devlp Neuroscience"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Thyroid hormone (T3) deficiency impairs the development of the CNS, particularly myelination. We have previously described an increase in the frequency of morphological abnormalities in the central myelin sheath in a hypothyroidism model, which reinforced the hypothesis of a role for T3 in myelin compaction. However, there are no data concerning the cellular distribution of myelin proteins in hypothyroid animals. In the present work, we describe the distribution of 2\u2032,3\u2032\u2010cyclic nucleotide 3\u2032\u2010phosphodiesterase (CNPase), myelin basic protein (MBP) and proteolipid protein (PLP) throughout the central myelin sheath of a hypothyroidism model. We used euthyroid and hypothyroid adult rats at 90 days of age. In order to induce hypothyroid status, animals received 0.02% methimazol from the 19th gestation day onwards. After perfusion with a fixative mixture, small pieces of corpus callosum were obtained, dehydrated and embedded in LR White resin. Ultrathin sections were immunoreacted, using specific antibodies revealed by a secondary antibody coupled to colloidal gold particles of 10 nm. Gold particle density per region of myelin sheath for each one of these proteins was obtained. In normal animals, CNPase, PLP and MBP were identified in sites that had already been described in previous studies. In hypothyroid animals, CNPase was identified in the region corresponding to compact lamellae, which normally does not contain this protein, while, in this same region, PLP and MBP immunolabeling were decreased. These results suggest that thyroid hormone deficiency impairs the distribution of the major oligodendrocyte\/myelin markers. This effect may justify the reduction in myelin sheath compaction previously demonstrated in a similar model of hypothyroidism.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.ijdevneu.2006.12.007","type":"journal-article","created":{"date-parts":[[2007,1,8]],"date-time":"2007-01-08T12:04:02Z","timestamp":1168257842000},"page":"87-94","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Ultrastructural identification of oligodendrocyte\/myelin proteins in corpus callosum of hypothyroid animals"],"prefix":"10.1002","volume":"25","author":[{"given":"A.A.","family":"Ferreira","sequence":"first","affiliation":[{"name":"Depto. Farmacologia e Psicobiologia Instituto de Biologia Roberto Alcantara Gomes Universidade do Estado do Rio de Janeiro 20551\u2010030 Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.J.S.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Depto. Ci\u00eancias Fisiol\u00f3gicas Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro 20551\u2010030 Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.C.","family":"Manh\u00e3es","sequence":"additional","affiliation":[{"name":"Depto. Ci\u00eancias Fisiol\u00f3gicas Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro 20551\u2010030 Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.C.","family":"Barradas","sequence":"additional","affiliation":[{"name":"Depto. Farmacologia e Psicobiologia Instituto de Biologia Roberto Alcantara Gomes Universidade do Estado do Rio de Janeiro 20551\u2010030 Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-5748(00)00028-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.1218"},{"issue":"1","key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1006\/exnr.1993.1080","article-title":"Appearance of PLP mRNA in specific regions of the developing rat lumbosacral spinal cord as revealed by in situ hybridization","volume":"121","author":"Baron P.","year":"1993","journal-title":"Exp. 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