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We observed that cells showed a fusiform shape with processes after 3 h treatment. Cell death was also observed when cells were incubated in the presence of the BCAA for 3 and 24 h. Val\u2010treated cells presented the most dramatic morphological alterations. Immunocytochemistry with anti\u2010actin and anti\u2010GFAP antibodies revealed that all BCAA induced reorganization of actin and GFAP cytoskeleton. Although phosphorylation regulates intermediate filament (IF) assembly\/disassembly, we verified that the BCAA did not change the in vitro phosphorylation of IF proteins either in C6 cells or in slices of cerebral cortex of rats during development (9\u2010, 12\u2010, 17\u2010 and 21\u2010day\u2010old). Furthermore, we observed that 3 h cell exposure to 5 mM of each BCAA resulted in a marked reduction of reduced glutathione (GSH) levels and significantly increased nitric oxide production. Finally, we observed that the morphological features caused by the BCAA on C6 cells were prevented by the use of the antioxidants GSH (1 mM) and\n                      <jats:italic>N<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>\u03c9<\/jats:italic>\n                      <\/jats:sup>\n                      \u2010nitro\u2010\n                      <jats:sc>l<\/jats:sc>\n                      \u2010arginine methyl ester (\n                      <jats:sc>l<\/jats:sc>\n                      \u2010NAME, 0.5 mM). On the basis of the present results, we conclude that free radical attack might be involved in the cell morphological alterations, as well as, in the cytoskeletal reorganization elicited by the BCAA. It is therefore presumed that these findings could be involved in the neuropathological features observed in patients affected by MSUD.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1016\/j.ijdevneu.2007.01.001","type":"journal-article","created":{"date-parts":[[2007,1,22]],"date-time":"2007-01-22T09:18:57Z","timestamp":1169457537000},"page":"181-189","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Branched\u2010chain amino acids accumulating in maple syrup urine disease induce morphological alterations in C6 glioma cells probably through reactive species"],"prefix":"10.1002","volume":"25","author":[{"given":"Priscila","family":"de Lima Pelaez","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udia","family":"Funchal","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samanta Oliveira","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana","family":"Heimfarth","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Zamoner","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmem","family":"Gottfried","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Latini","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir","family":"Wajner","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Pessoa\u2010Pureur","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041430116"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.161.3839.370"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-5748(03)00074-1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-005-4152-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715760400022350"},{"key":"e_1_2_6_7_1","first-page":"347","article-title":"Reduced glutathione disulfide","volume":"108","author":"Browne R.W.","year":"1998","journal-title":"Methods Mol. 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