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This inherited metabolic disease, caused by deficiency of the enzyme aspartoacylase, is clinically characterized by severe mental retardation, hypotonia and macrocephaly, and also generalized tonic and clonic type seizures in about half of the patients. Considering that the mechanisms of brain damage in this disease remain not fully understood, in the present study we investigated whether oxidative stress is elicited by <jats:italic>N<\/jats:italic>\u2010acetylaspartic acid. The <jats:italic>in vitro<\/jats:italic> effect of <jats:italic>N<\/jats:italic>\u2010acetylaspartic acid (10\u201380 mM) was studied on oxidative stress parameters: total radical\u2010trapping antioxidant potential (TRAP), total antioxidant reactivity (TAR), chemiluminescence, thiobarbituric acid\u2010reactive substances (TBA\u2010RS), reduced glutathione content, sufhydryl content and carbonyl content in the cerebral cortex of 14\u2010day\u2010old rats. The effect of the acute administration of <jats:italic>N<\/jats:italic>\u2010acetylaspartic acid (0.1\u20130.6 mmol\/g body weight) was studied on TRAP, TAR, carbonyl content, chemiluminescence and TBA\u2010RS. TRAP, TAR, reduced glutathione content and sulfhydryl content were significantly reduced, while chemiluminescence, TBA\u2010RS and carbonyl content were significantly enhanced by <jats:italic>N<\/jats:italic>\u2010acetylaspartic acid <jats:italic>in vitro<\/jats:italic>. The enhancement in TBA\u2010RS promoted by <jats:italic>N<\/jats:italic>\u2010acetylaspartic acid was completely prevented by ascorbic acid plus Trolox, and partially prevented by glutathione and dithiothreitol. The acute administration of <jats:italic>N<\/jats:italic>\u2010acetylaspartic acid also significantly reduced TRAP and TAR, and significantly enhanced carbonyl content, chemiluminescence and TBA\u2010RS. Our results indicate that <jats:italic>N<\/jats:italic>\u2010acetylaspartic acid promotes oxidative stress by stimulating lipid peroxidation, protein oxidation and by decreasing non\u2010enzymatic antioxidant defenses in rat brain. This could be another pathophysiological mechanism involved in Canavan Disease.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.ijdevneu.2007.04.002","type":"journal-article","created":{"date-parts":[[2007,5,8]],"date-time":"2007-05-08T13:33:55Z","timestamp":1178631235000},"page":"317-324","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["<i>N<\/i>\u2010Acetylaspartic acid promotes oxidative stress in cerebral cortex of rats"],"prefix":"10.1002","volume":"25","author":[{"given":"Carolina D.","family":"Pederzolli","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline P.","family":"Mescka","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Scapin","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francieli J.","family":"Rockenbach","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngela M.","family":"Sgaravatti","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirian B.","family":"Sgarbi","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela T.S.","family":"Wyse","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00f3vis M.D.","family":"Wannmacher","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir","family":"Wajner","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos S.","family":"Dutra\u2010Filho","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600 Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,7]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00687899"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1176\/ajp.150.9.1405"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(81)77050-2"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02028-X"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(01)01636-6"},{"issue":"1","key":"e_1_2_4_7_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.mce.2006.02.005","article-title":"Myocardial antioxidant enzyme activities and concentration and glutathione metabolism in experimental hyperthyroidism","volume":"249","author":"Araujo A.S.R.","year":"2006","journal-title":"Mol. 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