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A rat model with a down\u2010regulated <jats:italic>Mecp2<\/jats:italic> was established using a recombinant lentiviral vector expressing small hairpin RNA of the rat <jats:italic>Mecp2<\/jats:italic> gene. Four recombinant vectors were constructed by inserting sequences of small hairpin RNA targeting the rat <jats:italic>Mecp2<\/jats:italic> gene. To determine which vector resulted in optimal down\u2010regulation, rat skin fibroblasts were transfected with four recombinant vectors, respectively. The recombinant vector mecp2\u2010sh\u20101 decreased expression of <jats:italic>Mecp2<\/jats:italic> mRNA and protein relative to the Control group. 12\u201324 h after birth, neonatal rat pups were divided into three groups, Re\u2010Lenti group (injected with recombinant lentiviral vector, mecp2\u2010sh\u20101), Lenti group (injected with lentivirus containing no inserted sequences) and a non\u2010injected Control group (Control group). Rats in the injection groups were given intraventricular injections. 7 days and 21 days following injection, no inflammation was observed in the rat brain in the two injected groups, whereas EGFP expression was readily detectable. Sensory\u2010motor reflexes were transiently abnormal in Re\u2010Lenti group, whereas no abnormality was observed in either Control group. <jats:italic>Mecp2<\/jats:italic> mRNA was lower in the hippocampus and cerebral cortex in the Re\u2010Lenti group relative to the Lenti and Control groups. Although no typical RTT like symptoms were observed, the neonatal rats injected with recombinant lentivirus displayed some transient neurobehavioral abnormalities during early development. <jats:italic>Bdnf<\/jats:italic> mRNA expression decreased in the hippocampus, supporting the hypothesis that Bdnf may be a target gene of MeCP2 in the CNS.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.ijdevneu.2008.02.009","type":"journal-article","created":{"date-parts":[[2008,3,5]],"date-time":"2008-03-05T19:11:51Z","timestamp":1204744311000},"page":"457-465","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["RNAi\u2010induced down\u2010regulation of <i>Mecp2<\/i> expression in the rat brain"],"prefix":"10.1002","volume":"26","author":[{"given":"Jing","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Pediatrics Peking University First Hospital Beijing 100034 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