{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:48:07Z","timestamp":1791578887558,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T00:00:00Z","timestamp":1210809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J of Devlp Neuroscience"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Oligodendroglial cells differ in their ultrastructural appearance depending on their myelin producing and maintaining activity. To better understand the relationship between light and electron microscopic features of myelination, myelin formation in the corpus callosum was studied in young postnatal mice. Immunostaining for myelin basic protein (MBP), which has an important role in myelin compaction, was compared with conventional Luxol Fast Blue myelin staining and with electron microscopic images of unlabeled tissue.<\/jats:p><jats:p>MBP\u2010immunostaining labeled a few oligodendroglial cells at postnatal day (P)3, and a few axons at P7 in the corpus callosum, below the fronto\u2010parietal somatosensory cortex. By P10 there were more myelinated axons below the somatosensory cortex and the first MBP\u2010immunoreaction appeared in the cingulum: labeling appeared even later in the remaining areas of corpus callosum. Electron microscopy revealed numerous medium oligodendroglial cells at P7 in the corpus callosum, below the somatosensory cortex with the first sign of myelination at P10. By P14, there were numerous myelin sheaths with loosely built structure, and the number of myelin sheaths increased continuously thereafter. However, even as late as P28, the presence of both thick, compact and thin, loosely structured myelin sheaths in the same section suggested ongoing myelination. With Luxol Fast Blue myelin staining was first observed in the corpus callosum relatively late, at P14.<\/jats:p><jats:p>Areal differences in myelination of the corpus callosum, seen with MBP\u2010immunohistochemistry, indicate that myelin formation follows cortical maturation rather than the rostro\u2010caudal developmental growth of the corpus callosum. Myelination of the afferent and efferent fibers within the cortical areas seems to follow the inside\u2010out maturational pattern of cortical neurons, with the first myelinated axons always appearing in layers V\u2013VI.<\/jats:p><jats:p>In addition to the known neuronal and astroglial factors that regulate myelin formation by oligodendroglial cells, we suggest that these cells and their myelin covering may also influence axonal maturation. Light microscopic data obtained with MBP\u2010immunohistochemistry correlates well with electron microscopic observations but not with Luxol Fast Blue staining which reveals myelinated axons only relatively late in development. Therefore, both MBP\u2010immunostaining and electron microscopy are useful, alone or in combination, for the detection of myelination, demyelination as well as remyelination processes in animal models and also in humans.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.ijdevneu.2008.05.003","type":"journal-article","created":{"date-parts":[[2008,5,16]],"date-time":"2008-05-16T17:07:15Z","timestamp":1210957635000},"page":"575-584","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":88,"title":["A correlative light and electron microscopic study of postnatal myelination in the murine corpus callosum"],"prefix":"10.1002","volume":"26","author":[{"given":"Andr\u00e1s","family":"Vincze","sequence":"first","affiliation":[{"name":"Central Electron Microscopic Laboratory, Faculty of Medicine University of P\u00e9cs P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1ria","family":"M\u00e1zl\u00f3","sequence":"additional","affiliation":[{"name":"Central Electron Microscopic Laboratory, Faculty of Medicine University of P\u00e9cs P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Seress","sequence":"additional","affiliation":[{"name":"Central Electron Microscopic Laboratory, Faculty of Medicine University of P\u00e9cs P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e1muel","family":"Komoly","sequence":"additional","affiliation":[{"name":"Department of Neurology, Faculty of Medicine University of P\u00e9cs P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajnalka","family":"\u00c1brah\u00e1m","sequence":"additional","affiliation":[{"name":"Central Electron Microscopic Laboratory, Faculty of Medicine University of P\u00e9cs P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,5,15]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)90473-6"},{"key":"e_1_2_3_3_1","first-page":"487","article-title":"Normal postnatal development of the corpus callosum as demonstrated by MR imaging","volume":"9","author":"Barkovich A.J.","year":"1988","journal-title":"Am. J. Neuroradiol. (AJNR)"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.2.871"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199403000-00005"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00680-7"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2005.03.029"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9887"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200312012"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5369.1610"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490360202"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0387-7604(12)80271-3"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00810-9"},{"issue":"49","key":"e_1_2_3_14_1","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.neuron.2006.02.006","article-title":"Astrocytes promote myelination in response to electrical impulses","volume":"2006","author":"Ishibashi T.","year":"2006","journal-title":"Neuron"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00968708"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(88)90031-4"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1095"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.2007.00879.x"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902480304"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901650202"},{"key":"e_1_2_3_21_1","first-page":"109","volume-title":"Basic Neurochemistry: Molecular, Cellular and Medical Aspect","author":"Morell P.","year":"1989"},{"key":"e_1_2_3_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901390102"},{"key":"e_1_2_3_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg570"},{"key":"e_1_2_3_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200104025"},{"key":"e_1_2_3_25_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1983.0022"},{"key":"e_1_2_3_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(84)90012-2"},{"key":"e_1_2_3_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(79)91489-7"},{"issue":"1","key":"e_1_2_3_28_1","first-page":"25","article-title":"Neuronal migration and contact guidance in the primate telencephalon","volume":"54","author":"Rakic P.","year":"1978","journal-title":"Postgrad. Med. J."},{"key":"e_1_2_3_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01067791"},{"key":"e_1_2_3_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80110-0"},{"key":"e_1_2_3_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01002642"},{"key":"e_1_2_3_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.144.3616.309"},{"issue":"21","key":"e_1_2_3_33_1","doi-asserted-by":"crossref","first-page":"4381","DOI":"10.1242\/jcs.03242","article-title":"Neuron\u2010glia communication in the control of oligodendrocyte function and myelin biogenesis","volume":"119","author":"Simons M.","year":"2006","journal-title":"J. Cell Sci."},{"key":"e_1_2_3_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.5.2521"},{"key":"e_1_2_3_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01217922"},{"key":"e_1_2_3_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)01067-X"},{"key":"e_1_2_3_37_1","first-page":"67","article-title":"A quantitative electron microscopic study of myelination in the anterior limb of the anterior commissure of the mouse brain","volume":"119","author":"Sturrock R.R.","year":"1975","journal-title":"J. Anat."},{"key":"e_1_2_3_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1980.tb00219.x"},{"key":"e_1_2_3_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0387-7604(12)80124-0"},{"key":"e_1_2_3_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00686996"}],"container-title":["International Journal of Developmental Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0736574808000853?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0736574808000853?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.ijdevneu.2008.05.003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.ijdevneu.2008.05.003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1016\/j.ijdevneu.2008.05.003","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.ijdevneu.2008.05.003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T21:34:06Z","timestamp":1694036046000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/j.ijdevneu.2008.05.003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,15]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1016\/j.ijdevneu.2008.05.003"],"URL":"https:\/\/doi.org\/10.1016\/j.ijdevneu.2008.05.003","archive":["Portico"],"relation":{},"ISSN":["0736-5748","1873-474X"],"issn-type":[{"value":"0736-5748","type":"print"},{"value":"1873-474X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5,15]]},"assertion":[{"value":"2008-03-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}