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However, little is known about the mechanism of homocysteine on the degeneration of fetal brain. Thus, our study is aimed to investigate the effects of maternal hyperhomocysteinemia on oxidative stress and apoptosis in pup brain. Hyperhomocysteinemia was induced in female rats by way of administrating methionine dissolved in water at a dose of 1 g\/kg body weight throughout the pregnancy. After delivery, level of lipid peroxidation (LPO; as malondialdehyde + 4\u2010hydroxyalkenals) was determined in various fractions of pub brains. Furthermore, DNA fragmentation, levels of Bcl\u20102 protein and p53 mRNA expression were determined to evaluate apoptosis. Significant elevation was found in the levels of LPO in subcellular fractions of pup brains delivered from hyperhomocysteinemic mothers. DNA fragmentation, a hallmark of apoptosis was observed in the brain of pups of homocysteine group while significant reduction was seen in the levels of anti\u2010apoptotic Bcl\u20102 levels. In addition, maternal hyperhomocysteinemia increased cerebral p53 mRNA expression above the control value. As a conclusion, we demonstrate and suggest that the pups of hyperhomocysteinemic mothers have an increased oxidative stress in brain tissues. The increased oxidative stress appears to cause apoptosis and cell death. These results may be significant to understand chronic pathology of the complications of hyperhomocysteinemia and congenital malformations of fetuses.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2010.02.006","type":"journal-article","created":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T10:36:51Z","timestamp":1267180611000},"page":"325-329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Effects of maternal hyperhomocysteinemia induced by methionine intake on oxidative stress and apoptosis in pup rat brain"],"prefix":"10.1002","volume":"28","author":[{"given":"Sema T.","family":"Koz","sequence":"first","affiliation":[{"name":"Health Institute Bingol University 12000 Bingol Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nevhayat T.","family":"Gouwy","sequence":"additional","affiliation":[{"name":"Darussafaka Institute Department of Health Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nevgul","family":"Demir","sequence":"additional","affiliation":[{"name":"Kecioren Research Hospital Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor S.","family":"Nedzvetsky","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Biochemistry, Faculty of Biology Dnepropetrovsk National University Dnepropetrovsk Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebru","family":"Etem","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Firat University 23119 Elazig Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giyasettin","family":"Baydas","sequence":"additional","affiliation":[{"name":"Rectorate, Bingol University 12000 Bingol Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,2,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00448.2003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-918889"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297906130153"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.04.011"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.05.048"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079X.2003.02939.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2007.03.001"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2007.00506.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060339"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.3290"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i1.46"},{"key":"e_1_2_6_13_1","first-page":"9","article-title":"NF\u2010kappaB activation is associated with homocysteine\u2010induced injury in Neuro2a cells","volume":"7","author":"Ferlazzo N.","year":"2008","journal-title":"BMC Neurosci."},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490320108"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2002.01307.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00384.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10416"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/44.8.1833"},{"key":"e_1_2_6_19_1","first-page":"76","article-title":"Is metabolic evidence for vitamin B\u201012 and folate deficiency more frequent in elderly patients with Alzheimer's disease?","volume":"52","author":"Joosten E.","year":"1977","journal-title":"J. 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