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We also verified whether the alterations on the enzyme after administration of Met and\/or antioxidants were associated with changes in relative expression of Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase catalytic subunits (isoforms \u03b11, \u03b12 and \u03b13). For acute treatment, young rats received a single subcutaneous injection of Met or saline (control) and were sacrificed 12 h later. In another set of experiments, rats were pretreated for 1 week with daily intraperitoneal administration of vitamins E (40 mg\/kg) and C (100 mg\/kg) or saline. After that, rats received a single injection of Met or saline and were killed 12 h later. For chronic treatment, Met was administered to rats from the 6th to the 28th day of life; controls and treated rats were sacrificed 12 h after the last injection. In parallel to chronic treatment, rats received a daily intraperitoneal injection of vitamins E and C from the 6th to the 28th day of life and were killed 12 h after the last injection. Results showed that administration of antioxidants partially prevented the inhibition of enzyme activity caused by acute and chronic hypermethioninemia. Besides, we demonstrated that transcription of catalytic subunits of Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase was not altered by chronic and acute exposure to Met and\/or vitamins E plus C. These data strongly suggest the oxidative damage as one possible mechanism involved in the reduction of Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase activity caused by hypermethioninemia and if confirmed in human beings, we might propose the use of antioxidants as an adjuvant therapy in hypermethioninemic patients.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2011.02.001","type":"journal-article","created":{"date-parts":[[2011,2,26]],"date-time":"2011-02-26T12:37:03Z","timestamp":1298723823000},"page":"483-488","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Acute and chronic hypermethioninemia alter Na<sup>+<\/sup>,K<sup>+<\/sup>\u2010ATPase activity in rat hippocampus: prevention by antioxidants"],"prefix":"10.1002","volume":"29","author":[{"given":"Francieli M.","family":"Stefanello","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Neuroprote\u00e7\u00e3o e Doen\u00e7a Metab\u00f3lica 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Molecular Departamento de Biologia Celular e Molecular, Faculdade de Bioci\u00eancias Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul Avenida Ipiranga 6681 90619\u2010900 Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maira J.","family":"da Cunha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroprote\u00e7\u00e3o e Doen\u00e7a Metab\u00f3lica Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul Rua Ramiro Barcelos, 2600\u2010Anexo CEP 90035\u2010003 Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla D.","family":"Bonan","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroqu\u00edmica e Psicofarmacologia Departamento de Biologia Celular e Molecular, Faculdade de Bioci\u00eancias Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul Avenida Ipiranga 6681 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