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After 1 h home cage deprivation we observed an increase in the number of c\u2010Fos (neuronal activity marker) immunoreactive cells in several brain regions of the olfactory and stress\u2010related areas in normal neonates at 11 days. Next, pregnant rats were exposed to 50 mg\/kg of BrdU from gestation days 9\u201315, and their offspring at 11 days were home\u2010cage deprived. Compared to vehicle control, the number of c\u2010Fos immunoreactive cells in BrdU group was found to be decreased in the piriform cortex and locus coeruleus, which are known to play an important role in neonatal learning and memory. We also analyzed Pearson product\u2013moment correlation coefficient of the number of c\u2010Fos immunoreactive cells, focusing on the piriform cortex and locus coeruleus <jats:italic>versus<\/jats:italic> numerous other brain areas (11 areas including amygdala). Numerous significant correlations were observed in the vehicle control group, however, correlations of the locus coeruleus disappeared in the BrdU group. By observing c\u2010Fos immunoreactivity after home cage deprivation our study uncovers abnormal brain functions as early as postnatal day 11 in this disorder model. Based on these results, we propose a new histological approach for functional characterization of developmental disorder models.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2012.05.002","type":"journal-article","created":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T01:28:08Z","timestamp":1337218088000},"page":"507-515","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Abnormal brain function of the rat neonate in a prenatal 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdU)\u2010induced developmental disorder model"],"prefix":"10.1002","volume":"30","author":[{"given":"Tetsuo","family":"Ogawa","sequence":"first","affiliation":[{"name":"Department of Anatomy Showa University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makiko","family":"Kuwagata","sequence":"additional","affiliation":[{"name":"Department of Anatomy Showa University School of Medicine Tokyo 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