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In pancreas Wfs1 attenuates unfolded protein response (UPR) and protects cells from apoptosis. Loss of Wfs1 function results in Wolfram syndrome (OMIM 222300) characterized by early\u2010onset diabetes mellitus, progressive optic atrophy, diabetes insipidus, deafness, and psychiatric disorders. Similarly, <jats:italic>Wfs1<\/jats:italic>\u2212\/\u2212 mice exhibit diabetes and increased basal anxiety. In the adult central nervous system Wfs1 is prominent in central extended amygdala, striatum and hippocampus, brain structures largely involved in behavioral adaptation of the organism. Here, we describe the initiation pattern of <jats:italic>Wfs1<\/jats:italic> expression in mouse forebrain using mRNA <jats:italic>in situ<\/jats:italic> hybridization and compare it with <jats:italic>Synaptophysin<\/jats:italic> (<jats:italic>Syp1<\/jats:italic>), a gene encoding synaptic vesicle protein widely used as neuronal differentiation marker. We show that the expression of <jats:italic>Wfs1<\/jats:italic> starts during late embryonic development in the dorsal striatum and amygdala, then expands broadly at birth, possessing several transitory regions during maturation. <jats:italic>Syp1<\/jats:italic> expression precedes <jats:italic>Wfs1<\/jats:italic> and it is remarkably upregulated during the period of <jats:italic>Wfs1<\/jats:italic> expression initiation and maturation, suggesting relationship between neural activation and <jats:italic>Wfs1<\/jats:italic> expression. Using <jats:italic>in situ<\/jats:italic> hybridization and quantitative real\u2010time PCR we show that UPR\u2010related genes (<jats:italic>Grp78<\/jats:italic>, <jats:italic>Grp94<\/jats:italic>, and <jats:italic>Chop<\/jats:italic>) display dynamic expression in the perinatal brain when <jats:italic>Wfs1<\/jats:italic> is initiated and their expression pattern is not altered in the brain lacking functional Wfs1.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2014.03.009","type":"journal-article","created":{"date-parts":[[2014,3,30]],"date-time":"2014-03-30T13:48:52Z","timestamp":1396187332000},"page":"80-88","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Initiation and developmental dynamics of <i>Wfs1<\/i> expression in the context of neural differentiation and ER stress in mouse forebrain"],"prefix":"10.1002","volume":"35","author":[{"given":"Triin","family":"Tekko","sequence":"first","affiliation":[{"name":"Department of Physiology Institute of Biomedicine and Translational Medicine University of Tartu 19 Ravila Street 50411 Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kersti","family":"Lillev\u00e4li","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute of Biomedicine and Translational Medicine University of Tartu 19 Ravila Street 50411 Tartu Estonia"},{"name":"Department of Developmental Biology Institute of Molecular and Cell Biology Faculty of Science and Technology University of Tartu 46 Vanemuise Street 51014 Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendrik","family":"Luuk","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute of Biomedicine and Translational Medicine University of Tartu 19 Ravila Street 50411 Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silva","family":"S\u00fctt","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute of Biomedicine and Translational Medicine University of Tartu 19 Ravila Street 50411 Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Truu","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology Institute of Molecular and Cell Biology Faculty of Science and Technology University of Tartu 46 Vanemuise Street 51014 Tartu Estonia"},{"name":"Competence Centre for Cancer Research Akadeemia tee 15 Tallinn 12618 Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiit","family":"\u00d6rd","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology University of Tartu Riia 23 51010 Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e4rt","family":"M\u00f6ls","sequence":"additional","affiliation":[{"name":"Institute of Mathematical Statistics University of Tartu J. 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