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In order to verify the hypothesis that GAP\u201043 may influence the orientation of cell division through interacting with G proteins during neurogenesis, the GAP\u201043 RNA from adult C57 mouse was cloned into the pEGFP\u2010N1 vector, which was then transfected into Madin\u2013Darby Canine Kidney (MDCK) cells cultured in a three\u2010dimensional (3D) cell culture system. The interaction of GAP\u201043 with G\u03b1i was detected by co\u2010immunoprecipitation (co\u2010IP), while cystogenesis of 3D morphogenesis of MDCK cells and expression of GAP\u201043 and G\u03b1i were determined by immunofluorescence and Western blotting. The results showed are as follows: After being transfected by pEGFP\u2010N1\u2010GAP\u201043, GAP\u201043 was localized on the cell membrane and co\u2010localized with G\u03b1i, and this dramatically induced a defective cystogenesis in 3D morphogenesis of MDCK cells. The functional interaction between GAP\u201043 and G\u03b1i proteins was proven by the co\u2010IP assay. It can be considered from the results that the GAP\u201043 is involved in the orientation of cell division by interacting with G\u03b1i and this should be an important mechanism for neurogenesis in the mammalian brain.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2015.07.013","type":"journal-article","created":{"date-parts":[[2015,9,14]],"date-time":"2015-09-14T18:05:13Z","timestamp":1442253913000},"page":"333-339","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The influence of GAP\u201043 on orientation of cell division through G proteins"],"prefix":"10.1002","volume":"47","author":[{"given":"Rui","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurobiology Beijing Institute for Brain Disorders Beijing Center of Neural Regeneration and Repair Key Laboratory for Neurodegenerative Diseases of the Ministry of Education Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junpeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Beijing Institute for Brain Disorders Beijing Center of Neural Regeneration and Repair Key Laboratory for Neurodegenerative Diseases of the Ministry of Education Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Ju","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Beijing Institute for Brain Disorders Beijing Center of Neural Regeneration and Repair Key Laboratory for Neurodegenerative Diseases of the Ministry of Education Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujun","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Beijing Institute for Brain Disorders Beijing Center of Neural Regeneration and Repair Key Laboratory for Neurodegenerative Diseases of the Ministry of Education Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qunyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Beijing Institute for Brain Disorders Beijing Center of Neural Regeneration and Repair Key Laboratory for Neurodegenerative Diseases of the Ministry of Education Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.43.28478"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"9351","DOI":"10.1016\/S0021-9258(17)42705-0","article-title":"Activation of the inhibitory GTP\u2010binding protein of adenylate cyclase, Gi, by beta\u2010adrenergic receptors in reconstituted phospholipid vesicles","volume":"259","author":"Asano T.","year":"1984","journal-title":"J. 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