{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T04:43:35Z","timestamp":1792989815752,"version":"4.5.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,8,9]],"date-time":"2016-08-09T00:00:00Z","timestamp":1470700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J of Devlp Neuroscience"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The actions and responses of hypothalamic appetite regulatory factors change markedly during the neonatal to pre\u2010pubertal period in order to maintain appropriate metabolic and nutritional conditions. In this study, we examined the developmental changes in the hypothalamic mRNA levels of brain\u2010derived neurotrophic factor (BDNF), which is a potent anorectic factor and the changes in the sensitivity of the hypothalamic expression of this factor to fasting during the neonatal to pre\u2010pubertal period. Under fed conditions, hypothalamic BDNF mRNA expression decreased during development in both male and female rats. Similarly, the serum levels of leptin, which is a positive regulator of hypothalamic BDNF expression, also tended to fall during the developmental period. The serum leptin level and the hypothalamic BDNF mRNA level were found to be positively correlated in both sexes under the fed conditions. Hypothalamic BDNF mRNA expression was decreased by 24 h fasting (separating the rats from their mothers) in the early neonatal period (postnatal day 10) in both males and females, but no such changes were seen at postnatal day 20. Twenty\u2010four hours\u2019 fasting (food deprivation) did not affect hypothalamic BDNF mRNA expression in the pre\u2010pubertal period (postnatal day 30). On the other hand, the rats\u2019 serum leptin levels were decreased by 24 h fasting (separating the rats from their mothers at postnatal day 10 and 20, and food deprivation at postnatal day 30) throughout the early neonatal to pre\u2010pubertal period. The correlation between serum leptin and hypothalamic BDNF mRNA levels was not significant under the fasted conditions. It can be speculated that leptin partially regulates hypothalamic BDNF mRNA levels, but only in fed conditions. Such changes in hypothalamic BDNF expression might play a role in maintaining appropriate metabolic and nutritional conditions and promoting normal physical development. In addition, because maternal separation induces a negative energy balance and short\u2010 and long\u2010term stress responses, it is also possible that reductions in hypothalamic BDNF mRNA levels in the early neonatal period (postnatal day 10) may be partially induced by stress responses of the maternal deprivation.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2016.08.001","type":"journal-article","created":{"date-parts":[[2016,8,10]],"date-time":"2016-08-10T00:31:44Z","timestamp":1470789104000},"page":"1-5","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Developmental changes in the hypothalamic mRNA expression levels of brain\u2010derived neurotrophic factor and serum leptin levels: Their responses to fasting in male and female rats"],"prefix":"10.1002","volume":"54","author":[{"given":"Takeshi","family":"Iwasa","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Institute of Biomedical Sciences, Tokushima University Graduate School 3\u201018\u201015 Kuramoto\u2010Cho Tokushima 770\u20108503 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiya","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Institute of Biomedical Sciences, Tokushima University Graduate School 3\u201018\u201015 Kuramoto\u2010Cho Tokushima 770\u20108503 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyohito","family":"Yano","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Institute of Biomedical Sciences, Tokushima University Graduate School 3\u201018\u201015 Kuramoto\u2010Cho Tokushima 770\u20108503 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munkhsaikhan","family":"Munkhzaya","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Institute of Biomedical Sciences, Tokushima University Graduate 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