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In the dorsal root ganglia (DRG) the expression pattern of AT2R during development and the identity of the subpopulation expressing it remain unknown. Using a combination of semi\u2010quantitative PCR, western blotting and immunohistochemistry we examined the expression of AT2R at mRNA and protein levels in rat DRGs from embryonic day 15 (E15) until postnatal day 30 (PN30). We found that both AT2R mRNA and protein levels exhibited only minor (statistically non\u2010significant) fluctuations from E15 to PN30. Detailed quantitative analysis of ABC\/DAB AT2R staining showed a) that the receptor was present in most neurons at E15 and E18 and b) that postnatally it was predominantly expressed by small DRG neurons. Given that small neurons are putative C\u2010nociceptors and the proposed role of AT2R in neuropathic pain, we next examined whether these AT2R\u2010positive neurons co\u2010localized with Ret and trkA embryonically and with IB4\u2010binding postnatally. Most AT2R\u2010positive neurons expressed trkA embryonically and bound IB4 postnatally. We found strong positive statistically highly significant correlations between AT2R cytoplasmic%intensities and trkA at E15\/E18 and with Ret only at E18. Cytoplasmic AT2R also strongly and positively correlated with IB4\u2010binding at PN3, 15 and 30. Our demonstration that a subpopulation of C\u2010nociceptor\u2010like neurons expresses AT2R during development supports a role for this receptor in neuropathic pain.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2016.11.001","type":"journal-article","created":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T16:45:19Z","timestamp":1478277919000},"page":"10-17","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Nociceptor\u2010like rat dorsal root ganglion neurons express the angiotensin\u2010II AT2 receptor throughout development"],"prefix":"10.1002","volume":"56","author":[{"given":"Sergio","family":"Benitez","sequence":"first","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM) Facultad de Ciencias Medicas Universidad Nacional de Cuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Seltzer","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM) Facultad de Ciencias Medicas Universidad Nacional de Cuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian","family":"Acosta","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM) Facultad de Ciencias Medicas Universidad Nacional de Cuyo 5500 Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050442"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4528-13.2014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(03)03112-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1532-2149.2012.00269.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12990-015-0038-x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/1744806916654144"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1470320313503694"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1054\/jpai.2001.17697"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01469-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0061"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2013.04.002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0249-05.2005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.086199"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1072-06.2006"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/1470320312443720"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5930-09.2010"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069234"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","unstructured":"Jankowski M.P. 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