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However, the impact of this form of exposure on offspring vulnerability to stress is unknown.<\/jats:p><jats:p>The aim of this study was to evaluate the behavioural and epigenetic effects of stress in the offspring of male rats whose fathers were exposed to cannabinoids during adolescence. Male adolescent offspring of Win55,212\u20102 (1.2\u2009mg\/kg) treated rats were exposed during one week to variable stressors and subjected to behavioural tests of anxiety and episodic\u2010like memory, followed by an assessment of global DNA methylation and expression of DNA methyltransferases enzymes DNMT1 and DNMT3a mRNA in the prefrontal cortex.<\/jats:p><jats:p>Stress exposure induced a significant anxiogenic\u2010like effect but did not affect the episodic\u2010like memory in the offspring of Win55,212\u20102 exposed fathers in comparison to the offspring of non\u2010exposed fathers. These behavioural changes were subsequent to a significant increase in global DNA methylation and DNMT1 and DNMTa3 transcription in the prefrontal cortex.<\/jats:p><jats:p>These data suggest that the deleterious effect of chronic exposure to cannabinoids during adolescence are not limited to the exposed individuals but may increase the vulnerability to stress\u2010induced anxiety in the offspring and alter their epigenetic programming.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2018.11.007","type":"journal-article","created":{"date-parts":[[2018,11,23]],"date-time":"2018-11-23T09:48:48Z","timestamp":1542966528000},"page":"48-54","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Behavioural and epigenetic effects of paternal exposure to cannabinoids during adolescence on offspring vulnerability to stress"],"prefix":"10.1002","volume":"72","author":[{"given":"Zineb","family":"Ibn Lahmar Andaloussi","sequence":"first","affiliation":[{"name":"Physiology and Physiopathology Team Faculty of Sciences Genomic of Human Pathologies Research Centre, Mohammed V University in Rabat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid","family":"Taghzouti","sequence":"additional","affiliation":[{"name":"Physiology and Physiopathology Team Faculty of Sciences Genomic of Human Pathologies Research Centre, Mohammed V University in Rabat Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oualid","family":"Abboussi","sequence":"additional","affiliation":[{"name":"Institute of Academic Anaesthesia Division of Neuroscience Ninewells Hospital and Medical School, University of Dundee Dundee UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2014.02.014"},{"issue":"2","key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s11011-015-9753-2","article-title":"Behavioral effects of D3 receptor inhibition and 5\u2010HT4 receptor activation on animals undergoing chronic cannabinoid exposure during adolescence","volume":"31","author":"Abboussi O.","year":"2016","journal-title":"Metab. 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