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The N\u2010methyl\u2010D\u2010aspartate receptor (NMDAR) subunit GluN2B plays critical roles in cerebellar development, and methadone has been shown to possess NMDAR antagonist effect. Consequently, we wanted to explore if prenatal opioid exposure affected GluN2B subunit expression and NMDAR function in rat and chicken cerebellum. Pregnant rats were exposed to methadone (10\u202fmg\/kg\/day) or buprenorphine (1\u202fmg\/kg\/day) for the whole period of gestation, using an osmotic minipump. To further examine potential effects of prenatal opioid exposure in a limited time window, chicken embryos were exposed to a 20\u202fmg\/kg dose of methadone or morphine on embryonic days 13 and 14. Western blot analysis of cerebella isolated from 14 days old rat pups exposed to buprenorphine showed significantly lower level of the GluN2B subunit, while the opioid exposed chicken embryo cerebellar GluN2B expression remained unaffected at embryonic day 17. However, we observed increased NMDA\/glycine\u2010induced calcium influx in cerebellar granule neurone cultures from opioid exposed chicken embryos. We conclude that prenatal opioid exposure leads to opioid receptor\u2010dependent reduction in the postnatal expression of GluN2B in rat cerebella, and increase in NMDA\u2010induced calcium influx in chicken embryo cerebella.<\/jats:p>","DOI":"10.1016\/j.ijdevneu.2019.07.009","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T15:25:21Z","timestamp":1563981921000},"page":"19-27","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Opioid receptor\u2010mediated changes in the NMDA receptor in developing rat and chicken"],"prefix":"10.1002","volume":"78","author":[{"given":"Marthe Fredheim","family":"Fjelldal","sequence":"first","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences Department of Pharmacy University of Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mussie Ghezu","family":"Hadera","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences Department of Pharmacy University of Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Kongstorp","sequence":"additional","affiliation":[{"name":"Section for Drug Abuse Research Department of Forensic Sciences Oslo University Hospital Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars Peter Engeset","family":"Austdal","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences Department of Pharmacy University of Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"\u0160ulovi\u0107","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences Department of Pharmacy University of Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannike M\u00f8rch","family":"Andersen","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences Department of Pharmacy University of Oslo Norway"},{"name":"Section for Drug Abuse Research Department of Forensic Sciences Oslo University Hospital Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ragnhild Elisabeth","family":"Paulsen","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Biosciences Department of Pharmacy University of Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903470112"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200503150-00017"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12438"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10550887.2012.694598"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2006.09.006"},{"issue":"6","key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1159\/000111918","article-title":"Developmental profile of kappa, mu and Delta Opioid receptors in the rat and Guinea pig cerebellum","volume":"11","author":"Barg J.","year":"1989","journal-title":"Dev. 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