{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:45:10Z","timestamp":1792161910911,"version":"4.4.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2004,7,1]],"date-time":"2004-07-01T00:00:00Z","timestamp":1088640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Food Microbiology"],"published-print":{"date-parts":[[2004,7]]},"DOI":"10.1016\/j.ijfoodmicro.2004.01.018","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T07:03:15Z","timestamp":1084431795000},"page":"175-183","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":84,"title":["Sperm bioassay for rapid detection of cereulide-producing Bacillus cereus in food and related environments"],"prefix":"10.1016","volume":"94","author":[{"given":"Maria A.","family":"Andersson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elina L.","family":"J\u00e4\u00e4skel\u00e4inen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranad","family":"Shaheen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuula","family":"Pirhonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc M.","family":"Wijnands","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirja S.","family":"Salkinoja-Salonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB1","first-page":"31","article-title":"A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacuole formation in HEp-2 cells","volume":"121","author":"Agata","year":"1994","journal-title":"FEMS Microbiol. Lett."},{"issue":"1","key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB2","first-page":"17","article-title":"A novel dodecadepsipeptide, cereulide, is an emetic toxin of Bacillus cereus","volume":"129","author":"Agata","year":"1995","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s002849900076","article-title":"Production of an emetic toxin, cereulide, is associated with a specific class of Bacillus cereus","volume":"33","author":"Agata","year":"1996","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB4","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0168-1605(01)00692-4","article-title":"Production of Bacillus cereus emetic toxin (cereulide) in various foods","volume":"73","author":"Agata","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB5","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1128\/aem.63.2.387-393.1997","article-title":"Bacteria, molds and toxins in water damaged building materials","volume":"63","author":"Andersson","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB6","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1128\/AEM.64.4.1338-1343.1998","article-title":"A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores","volume":"64","author":"Andersson","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB7","first-page":"119","article-title":"Bacillus cereus","volume":"vol. 1","author":"Batt","year":"2000"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB8","first-page":"141","article-title":"Detection of toxins","volume":"vol. 1","author":"Beattie","year":"2000"},{"issue":"4","key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB9","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1128\/AEM.65.4.1811-1812.1999","article-title":"Semiautomated metabolic staining assay for Bacillus cereus emetic toxin","volume":"65","author":"Finlay","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB10","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1046\/j.1472-765x.2000.00835.x","article-title":"Bacillus cereus produces most emetic toxin at lower temperatures","volume":"31","author":"Finlay","year":"2000","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB11","series-title":"Foodborne Diseases","first-page":"261","article-title":"Bacillus cereus food poisoning","author":"Griffiths","year":"2002"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB12","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0887-2333(01)00008-X","article-title":"Factors influencing nominal effective concentrations of chemical compounds in vitro: cell concentration","volume":"15","author":"G\u00fclden","year":"2001","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB13","doi-asserted-by":"crossref","first-page":"2479","DOI":"10.1128\/AEM.68.5.2479-2483.2002","article-title":"Quantitative analysis of cereulide, the emetic toxin of Bacillus cereus, produced under different conditions","volume":"68","author":"H\u00e4ggblom","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB14","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/S0887-2333(03)00097-3","article-title":"A new method for in vitro detection of microbially produced mitochondrial toxins","volume":"17","author":"Hoornstra","year":"2003","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB15","series-title":"Dried Milk Products. Enumeration of Bacillus cereus. Most Probable Number Technique. Standard 181","author":"IDF (International Dairy Federation)","year":"1998"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB16","series-title":"Microbiology, General Guidance for the Enumeration of Bacillus cereus, Colony Count Technique at 30 \u00b0C","author":"ISO (International Organisation for Standardisation)","year":"1993"},{"issue":"6","key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB17","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.4315\/0362-028X-66.6.1047","article-title":"Potential of bakery products for producing cereulide, the Bacillus cereus emetic toxin: quantitative analysis by chemical and biological methods","volume":"66","author":"J\u00e4\u00e4skel\u00e4inen","year":"2003","journal-title":"J. Food Prot."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB18","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/S0887-2333(03)00096-1","article-title":"In vitro assay for human toxicity of cereulide, the emetic toxin produced by food poisoning Bacillus cereus","volume":"17","author":"J\u00e4\u00e4skel\u00e4inen","year":"2003","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB19","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S1286-4579(00)00269-0","article-title":"Epidemiology and pathogenesis of Bacillus cereus infection","volume":"2","author":"Kotiranta","year":"2000","journal-title":"Microbes Infect."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB20","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1056\/NEJM199704173361604","article-title":"Fulminant liver failure in association with the emetic toxin of Bacillus cereus","volume":"336","author":"Mahler","year":"1997","journal-title":"New Engl. J. Med."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB21","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1023\/A:1002706906154","article-title":"Prevalence and expression of enterotoxins in Bacillus cereus and other Bacillus spp., a literature review","volume":"77","author":"McKillip","year":"2000","journal-title":"Antonie van Leeuwenhoek"},{"issue":"1","key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB22","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1046\/j.1432-1327.1999.00476.x","article-title":"Ionophoretic properties and mitochondrial effects of cereulide: the emetic toxin of B. cereus","volume":"263","author":"Mikkola","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB23","series-title":"Bacillus cereus. Determination in Foods. UDC 579.852.11. Method No. 67","year":"1997"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB24","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1046\/j.1365-2249.2002.01898.x","article-title":"Inhibition of human natural killer cell activity by cereulide, an emetic toxin from Bacillus cereus","volume":"129","author":"Paananen","year":"2002","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB25","series-title":"A Colour Atlas of Bacillus Species","first-page":"98","author":"Parry","year":"1983"},{"issue":"1","key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB26","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0723-2020(99)80036-8","article-title":"Evaluation of methods for recognizing strains of the Bacillus cereus group with food poisoning potential among industrial and environmental contaminants","volume":"22","author":"Pirttij\u00e4rvi","year":"1999","journal-title":"Syst. Appl. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB27","series-title":"Food and Drug Administration, Bacteriological Analytical Manual","first-page":"1401","article-title":"Bacillus cereus","author":"Rhodehamel","year":"1998"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB28","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0887-2333(02)00014-0","article-title":"Factors influencing nominal effective concentrations of chemical compounds in vitro: medium protein concentration","volume":"16","author":"Seibert","year":"2002","journal-title":"Toxicol. In Vitro"},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB29","first-page":"89","article-title":"Serology and characterization of toxigenic Bacillus cereus","volume":"47","author":"Shinagawa","year":"1993","journal-title":"Neth. Milk Dairy J."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB30","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1093\/ajcn\/32.1.219","article-title":"Properties and production characteristics of vomiting, diarrheal and necrotic toxins of Bacillus cereus","volume":"32","author":"Turnbull","year":"1979","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.ijfoodmicro.2004.01.018_BIB31","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1128\/aem.63.1.44-49.1997","article-title":"Antimycoplasma properties and application in cell culture of surfactin, a lipopeptide antibiotic from Bacillus subtilis","volume":"64","author":"Vollenbroich","year":"1997","journal-title":"Appl. Environ. Microbiol."}],"container-title":["International Journal of Food Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168160504000753?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168160504000753?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T11:15:29Z","timestamp":1759144529000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168160504000753"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,7]]}},"alternative-id":["S0168160504000753"],"URL":"https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2004.01.018","relation":{},"ISSN":["0168-1605"],"issn-type":[{"value":"0168-1605","type":"print"}],"subject":[],"published":{"date-parts":[[2004,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sperm bioassay for rapid detection of cereulide-producing Bacillus cereus in food and related environments","name":"articletitle","label":"Article Title"},{"value":"International Journal of Food Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2004.01.018","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2004 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}