{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:32:53Z","timestamp":1791304373849,"version":"4.3.1"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Food Microbiology"],"published-print":{"date-parts":[[2011,3]]},"DOI":"10.1016\/j.ijfoodmicro.2010.04.006","type":"journal-article","created":{"date-parts":[[2010,4,12]],"date-time":"2010-04-12T03:28:23Z","timestamp":1271042903000},"page":"S145-S151","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"S1","title":["An innovative molecular detection tool for tracking and tracing Clostridium botulinum types A, B, E, F and other botulinum neurotoxin producing Clostridia based on the GeneDisc cycler"],"prefix":"10.1016","volume":"145","author":[{"given":"P.","family":"Fach","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Fenicia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Knutsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.R.","family":"Wielinga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Anniballi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Delibato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Auricchio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Woudstra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"\u00c5gren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Segerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"de Medici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.J.","family":"van Rotterdam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0005","doi-asserted-by":"crossref","first-page":"4342","DOI":"10.1128\/JCM.43.9.4342-4348.2005","article-title":"Improvement in laboratory diagnosis of wound botulism and tetanus among injecting illicit-drug users by use of real-time PCR assays for neurotoxin gene fragments","volume":"43","author":"Akbulut","year":"2005","journal-title":"Journal of Clinical Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0010","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1001\/jama.285.8.1059","article-title":"Botulinum toxin as a biological weapon: medical and public health management","volume":"285","author":"Arnon","year":"2001","journal-title":"Journal of the American Medical Association"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0015","doi-asserted-by":"crossref","first-page":"3589","DOI":"10.1128\/JCM.01192-07","article-title":"First case of type E wound botulism diagnosed using real-time PCR","volume":"45","author":"Artin","year":"2007","journal-title":"Journal of Clinical Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0020","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1111\/j.1365-2672.2008.04076.x","article-title":"Evaluation of the \u2018GeneDisc\u2019 real-time PCR system for detection of enterohaemorrhagic Escherichia coli (EHEC) O26, O103, O111, O145 and O157 strains according to their virulence markers and their O- and H-antigen-associated genes","volume":"106","author":"Beutin","year":"2009","journal-title":"Journal of Applied Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0025","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4315\/0362-028X-64.2.201","article-title":"Screening for Clostridium botulinum type A, B, and E in cooked chilled foods containing vegetables and raw material using polymerase chain reaction and molecular probes","volume":"64","author":"Braconnier","year":"2001","journal-title":"Journal of Food Protection."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0030","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0168-1605(03)00371-4","article-title":"Prevalence of Clostridium botulinum in food raw materials used in REPFEDs manufactured in France","volume":"91","author":"Carlin","year":"2004","journal-title":"International Journal of Food Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0035","first-page":"1","article-title":"Case definition for infectious conditions under public health surveillance","volume":"46","author":"CDC","year":"1997","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0040","series-title":"Handbook for Epidemiologists, Clinical and Laboratory workers","article-title":"Botulism in United States, 1899\u20131966","author":"CDC","year":"1998"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0045","doi-asserted-by":"crossref","first-page":"8643","DOI":"10.1128\/JB.00784-07","article-title":"Sequencing the botulinum neurotoxin gene and related genes in Clostridium botulinum type E strains reveals orfx3 and a novel type E neurotoxin subtype","volume":"189","author":"Chen","year":"2007","journal-title":"Journal of Bacteriology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0050","doi-asserted-by":"crossref","first-page":"6132","DOI":"10.1128\/AEM.00469-08","article-title":"Quantitative real-time reverse transcription-PCR analysis reveals stable and prolonged neurotoxin cluster gene activity in a Clostridium botulinum type E strain at refrigeration temperature","volume":"74","author":"Chen","year":"2008","journal-title":"Applied and Environmental Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0055","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0168-1605(00)00197-5","article-title":"Effect of temperature on growth characteristics of Bacillus cereus TZ415","volume":"55","author":"Choma","year":"2000","journal-title":"International Journal of Food Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0060","doi-asserted-by":"crossref","first-page":"6457","DOI":"10.1128\/AEM.00805-09","article-title":"Multiplex PCR for detection of botulinum neurotoxin-producing clostridia in clinical, food, and environmental samples","volume":"75","author":"De Medici","year":"2009","journal-title":"Applied and Environmental. Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0065","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/j.1365-2672.1993.tb02771.x","article-title":"Polymerase chain reaction for the rapid identification of Clostridium botulinum type A strains and detection in food samples","volume":"75","author":"Fach","year":"1993","journal-title":"Journal of Applied Bacteriology"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0070","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1128\/aem.61.1.389-392.1995","article-title":"PCR and gene probe identification of botulinum neurotoxin A-, B-, E-, F-, and G-producing Clostridium spp. and evaluation in food samples","volume":"61","author":"Fach","year":"1995","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0075","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1111\/j.1365-2672.2009.04215.x","article-title":"Development of real-time PCR tests for detecting botulinum neurotoxins A, B, E, F producing Clostridium botulinum, Clostridium baratii and Clostridium butyricum","volume":"107","author":"Fach","year":"2009","journal-title":"Journal of Applied Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0080","first-page":"134","article-title":"Infant botulism","volume":"45","author":"Fenicia","year":"2009","journal-title":"Annali dell' Istituto Superiore Di Sanit\u00e0"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0085","doi-asserted-by":"crossref","first-page":"2891","DOI":"10.1128\/AEM.02234-06","article-title":"SYBR green real-time PCR method to detect Clostridium botulinum type A","volume":"73","author":"Fenicia","year":"2007","journal-title":"Applied and Environmental Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0090","series-title":"Botulinum and Tetanus Neurotoxins","first-page":"421","article-title":"Serological subtypes of botulinal toxins","author":"Gimenez","year":"1993"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0095","first-page":"55","article-title":"Botulism: the present status of the disease","volume":"195","author":"Hatheway","year":"1995","journal-title":"Current Topics in Microbiology and Immunology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0100","first-page":"202","article-title":"Pathophysiological properties of clostridial neurotoxins","volume":"vol. 2","author":"Herreros","year":"1999"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0105","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1128\/JB.01180-06","article-title":"Genetic diversity among Botulinum Neurotoxin-producing clostridial strains","volume":"189","author":"Hill","year":"2007","journal-title":"Journal of Bacteriology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0110","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1017\/S0950268898008693","article-title":"Prevalence of Clostridium botulinum type E in Finnish fish and fishery products","volume":"120","author":"Hyyti\u00e4","year":"1998","journal-title":"Epidemiology and Infection"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0115","first-page":"79","article-title":"Clostridium botulinum type E in Bavarian fish","volume":"50","author":"Hyyti\u00e4-Trees","year":"1999","journal-title":"Archiv f\u00fcr Lebensmittelhygiene"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0120","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/CMR.19.2.298-314.2006","article-title":"Laboratory diagnostics of botulism","volume":"19","author":"Lindstrom","year":"2006","journal-title":"Clinical Microbiology Review"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0125","doi-asserted-by":"crossref","first-page":"5694","DOI":"10.1128\/AEM.67.12.5694-5699.2001","article-title":"Multiplex PCR assay for detection and identification of Clostridium botulinum types A, B, E, and F in food and fecal material","volume":"67","author":"Lindstrom","year":"2001","journal-title":"Applied and Environmental Microbiology."},{"issue":"183\u2013265","key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0130","first-page":"320","article-title":"Biology and genomic analysis of Clostridium botulinum","volume":"55","author":"Peck","year":"2009","journal-title":"Advances in Microbial Physiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0135","first-page":"1","article-title":"Ecology of neurotoxigenic strains of Clostridia","volume":"vol. 195","author":"Popoff","year":"1995"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0140","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.mimet.2007.09.016","article-title":"Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A\u2013G) gene complex","volume":"71","author":"Raphael","year":"2007","journal-title":"Journal of Microbiological Methods."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0145","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1007\/s002849900299","article-title":"Gene organization and sequence determination of the two botulinum neurotoxin gene clusters in Clostridium botulinum type A(B) strain NCTC 2916","volume":"36","author":"Rodriguez Jovita","year":"1998","journal-title":"Current Microbiology"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0150","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1099\/jmm.0.012567-0","article-title":"A quadraplexed real-time PCR assay for rapid detection and differentiation of the Clostridium botulinum toxin genes A, B, E and F","volume":"59","author":"Satterfield","year":"2010","journal-title":"Journal of Medical Microbiology."},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0155","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1152\/physrev.2000.80.2.717","article-title":"Neurotoxins affecting neuroexocytosis","volume":"80","author":"Schiavo","year":"2000","journal-title":"Physiological Reviews"},{"key":"10.1016\/j.ijfoodmicro.2010.04.006_bb0160","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1128\/AEM.02399-06","article-title":"Integrated real-time PCR for detection and monitoring of Legionella. pneumophila in water systems","volume":"73","author":"Yaradou","year":"2007","journal-title":"Applied and Environmental Microbiology"}],"container-title":["International Journal of Food Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168160510001911?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168160510001911?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T11:39:51Z","timestamp":1759145991000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168160510001911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3]]},"references-count":32,"alternative-id":["S0168160510001911"],"URL":"https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2010.04.006","relation":{},"ISSN":["0168-1605"],"issn-type":[{"value":"0168-1605","type":"print"}],"subject":[],"published":{"date-parts":[[2011,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An innovative molecular detection tool for tracking and tracing Clostridium botulinum types A, B, E, F and other botulinum neurotoxin producing Clostridia based on the GeneDisc cycler","name":"articletitle","label":"Article Title"},{"value":"International Journal of Food Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2010.04.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}