{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T20:59:40Z","timestamp":1768683580592,"version":"3.49.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Food Microbiology"],"published-print":{"date-parts":[[2019,11]]},"DOI":"10.1016\/j.ijfoodmicro.2019.108289","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T19:40:55Z","timestamp":1565120455000},"page":"108289","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"special_numbering":"C","title":["Characterization of Listeria monocytogenes serovar 1\/2a, 1\/2b, 1\/2c and 4b by high resolution melting analysis for epidemiological investigations"],"prefix":"10.1016","volume":"310","author":[{"given":"Manuela","family":"Tamburro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela Lucia","family":"Sammarco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Incoronata","family":"Fanelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9953-7769","authenticated-orcid":false,"given":"Giancarlo","family":"Ripabelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0005","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.copbio.2017.11.002","article-title":"Genomics of foodborne pathogens for microbial food safety","volume":"49","author":"Allard","year":"2018","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb2019","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1186\/s12879-017-2441-6","article-title":"Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy - 2009-2011.","volume":"17","author":"Amato","year":"2017","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0010","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s12223-009-0003-z","article-title":"Subtyping of Listeria monocytogenes isolates by actA gene sequencing, PCR-fingerprinting, and cell-invasion assay","volume":"54","author":"Bania","year":"2009","journal-title":"Folia Microbiol. (Praha)"},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0015","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00969-14","article-title":"Comparison of widely used Listeria monocytogenes strains EGD, 10403S, and EGD-e highlights genomic variations underlying differences in pathogenicity","volume":"5","author":"B\u00e9cavin","year":"2014","journal-title":"MBio"},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.foodcont.2016.12.016","article-title":"A review of Listeria monocytogenes: an update on outbreaks, virulence, dose-response, ecology, and risk assessments","volume":"75","author":"Buchanan","year":"2017","journal-title":"Food Control"},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0025","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/S0966-842X(02)02342-9","article-title":"Surface proteins and the pathogenic potential of Listeria monocytogenes","volume":"10","author":"Cabanes","year":"2002","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0030","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1089\/fpd.2015.2115","article-title":"The continuous challenge of characterizing the foodborne pathogen Listeria monocytogenes","volume":"13","author":"Camargo","year":"2016","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0035","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/S1473-3099(16)30521-7","article-title":"Clinical features and prognostic factors of listeriosis: the MONALISA national prospective cohort study","volume":"17","author":"Charlier","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0040","article-title":"Genomic characterization helps dissecting an outbreak of listeriosis in Northern Italy","volume":"9","author":"Comandatore","year":"2017","journal-title":"PLoS Curr"},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0045","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/S1473-3099(14)70870-9","article-title":"The global burden of listeriosis: a systematic review and meta-analysis","volume":"14","author":"de Noordhout","year":"2014","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0050","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1186\/1471-2148-8-277","article-title":"Lineage specific recombination rates and microevolution in Listeria monocytogenes","volume":"8","author":"den Bakker","year":"2008","journal-title":"BMC Evol. Biol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0055","doi-asserted-by":"crossref","first-page":"6085","DOI":"10.1128\/AEM.00447-10","article-title":"A population genetics-based and phylogenetic approach to understanding the evolution of virulence in the genus Listeria","volume":"76","author":"den Bakker","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0060","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1139\/cjm-2015-0281","article-title":"Phylogenomic grouping of Listeria monocytogenes","volume":"61","author":"Doijad","year":"2015","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0065","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1128\/JCM.42.8.3819-3822.2004","article-title":"Differentiation of the major Listeria monocytogenes serovars by multiplex PCR","volume":"42","author":"Doumith","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0070","doi-asserted-by":"crossref","first-page":"5500","DOI":"10.2903\/j.efsa.2018.5500","article-title":"The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017","volume":"16","author":"European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA and ECDC)","year":"2018","journal-title":"EFSA J."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0075","doi-asserted-by":"crossref","first-page":"810","DOI":"10.4315\/0362-028X.JFP-15-428","article-title":"A rapid multiplex real-time PCR high-resolution melt curve assay for the simultaneous detection of Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus in food","volume":"79","author":"Forghani","year":"2016","journal-title":"J. Food Prot."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0080","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s00284-018-1458-4","article-title":"Genetic analysis of the Listeria pathogenicity island 1 of Listeria monocytogenes 1\/2a and 4b isolates","volume":"75","author":"Hadjilouka","year":"2018","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0085","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.tube.2014.12.007","article-title":"Whole genome sequencing: a new paradigm in the surveillance and control of human tuberculosis","volume":"95","author":"Hasnain","year":"2015","journal-title":"Tuberculosis (Edinb.)"},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0090","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/jcm.26.11.2465-2466.1988","article-title":"Numerical index of the discriminatory ability of typing systems: an application of Simpson\u2019s index of diversity","volume":"26","author":"Hunter","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0095","doi-asserted-by":"crossref","first-page":"2094","DOI":"10.1086\/420853","article-title":"A molecular marker for evaluating the pathogenic potential of foodborne Listeria monocytogenes","volume":"189","author":"Jacquet","year":"2004","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0100","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/j.1574-6968.2012.02535.x","article-title":"Rapid molecular identification of Listeria species by use of real-time PCR and high-resolution melting analysis","volume":"330","author":"Jin","year":"2012","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0105","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1128\/JCM.02344-15","article-title":"Prospective whole-genome sequencing enhances national surveillance of Listeria monocytogenes","volume":"54","author":"Kwong","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0110","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.meegid.2015.08.008","article-title":"The evolution and epidemiology of Listeria monocytogenes in Europe and the United States","volume":"35","author":"Lomonaco","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0115","doi-asserted-by":"crossref","first-page":"e01276","DOI":"10.1128\/genomeA.01276-17","article-title":"Draft genome sequences of 510 Listeria monocytogenes strains from food isolates and human listeriosis cases from Northern Italy","volume":"6","author":"Lomonaco","year":"2018","journal-title":"Genome Announc."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0120","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00541-17","article-title":"Spontaneous loss of virulence in natural populations of Listeria monocytogenes","volume":"85","author":"Maury","year":"2017","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0125","doi-asserted-by":"crossref","first-page":"M2868","DOI":"10.1111\/1750-3841.13133","article-title":"Listeria monocytogenes: strain heterogeneity, methods, and challenges of subtyping","volume":"80","author":"Nyarko","year":"2015","journal-title":"J. Food Sci."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0130","doi-asserted-by":"crossref","first-page":"e107342","DOI":"10.1371\/journal.pone.0099223","article-title":"Establishment of a simple and rapid identification method for Listeria spp. by using high-resolution melting analysis, and its application in food industry","volume":"9","author":"Ohshima","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0135","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.ijfoodmicro.2017.04.015","article-title":"A novel typing method for Listeria monocytogenes using high-resolution melting analysis (HRMA) of tandem repeat regions","volume":"253","author":"Ohshima","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0140","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.meegid.2008.04.006","article-title":"Lineage specific recombination and positive selection in coding and intragenic regions contributed to evolution of the main Listeria monocytogenes virulence gene cluster","volume":"8","author":"Orsi","year":"2008","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0145","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijmm.2010.05.002","article-title":"Listeria monocytogenes lineages: genomics, evolution, ecology, and phenotypic characteristics","volume":"301","author":"Orsi","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0150","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jmoldx.2010.11.002","article-title":"Gene scanning of an internalin B gene fragment using high-resolution melting curve analysis as a tool for rapid typing of Listeria monocytogenes","volume":"13","author":"Pietzka","year":"2011","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0155","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.3389\/fmicb.2018.01103","article-title":"Virulence gene sequencing highlights similarities and differences in sequences in Listeria monocytogenes serotype 1\/2a and 4b strains of clinical and food origin from 3 different geographic locations","volume":"9","author":"Poimenidou","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0160","doi-asserted-by":"crossref","first-page":"e1000146","DOI":"10.1371\/journal.ppat.1000146","article-title":"A new perspective on Listeria monocytogenes evolution","volume":"4","author":"Ragon","year":"2008","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0165","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.mimet.2016.09.015","article-title":"A multiplex PCR for detection of Listeria monocytogenes and its lineages","volume":"130","author":"Rawool","year":"2016","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb2018","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.1128\/AEM.70.4.2180-2185.2004","article-title":"Use of PCR-restriction fragment length polymorphism of inlA for rapid screening of Listeria monocytogenes strains deficient in the ability to invade Caco-2 cells.","volume":"70","author":"Rousseaux","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0170","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1080\/13102818.2014.901681","article-title":"Genotyping of Listeria monocytogenes isolates from poultry carcasses using high resolution melting (HRM) analysis","volume":"28","author":"Sakaridis","year":"2014","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0175","first-page":"10","article-title":"Molecular epidemiology of infectious diseases: analytical methods and results interpretation","volume":"26","author":"Sammarco","year":"2014","journal-title":"Ann. Ig."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0180","doi-asserted-by":"crossref","first-page":"6024","DOI":"10.1128\/AEM.01049-15","article-title":"Whole-genome sequencing allows for improved identification of persistent Listeria monocytogenes in food-associated environments","volume":"81","author":"Stasiewicz","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0185","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.ijfoodmicro.2016.12.009","article-title":"A rapid typing method for Listeria monocytogenes based on high-throughput multilocus sequence typing (Hi-MLST)","volume":"243","author":"Takahashi","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0190","first-page":"293","article-title":"High resolution melting as a rapid, reliable, accurate and cost-effective emerging tool for genotyping pathogenic bacteria and enhancing molecular epidemiological surveillance: a comprehensive review of the literature","volume":"29","author":"Tamburro","year":"2017","journal-title":"Ann. Ig."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0195","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1111\/j.1365-2672.2009.04555.x","article-title":"Typing of Listeria monocytogenes strains isolated in Italy by inlA gene characterization and evaluation of a new cost-effective approach to antisera selection for serotyping","volume":"108","author":"Tamburro","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0200","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.cimid.2015.03.004","article-title":"Gene expression in Listeria monocytogenes exposed to sublethal concentration of benzalkonium chloride","volume":"40","author":"Tamburro","year":"2015","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0205","doi-asserted-by":"crossref","first-page":"fnv018","DOI":"10.1093\/femsle\/fnv018","article-title":"Evaluation of transcription levels of inlA, inlB, hly, bsh and prfA genes in Listeria monocytogenes strains using quantitative reverse-transcription PCR and ability of invasion into human CaCo-2 cells","volume":"362","author":"Tamburro","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0210","author":"Tamburro","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0215","doi-asserted-by":"crossref","first-page":"3418","DOI":"10.1128\/JCM.01709-12","article-title":"Microbiological applications of high-resolution melting analysis","volume":"50","author":"Tong","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0220","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.meegid.2006.01.004","article-title":"Listeria monocytogenes internalins are highly diverse and evolved by recombination and positive selection","volume":"6","author":"Tsai","year":"2006","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0225","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1139\/W10-054","article-title":"Rapid identification of Listeria species and screening for variants by melting curve and high-resolution melting curve analyses of the intergenic spacer region of the rRNA gene","volume":"56","author":"Wang","year":"2010","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0230","doi-asserted-by":"crossref","first-page":"4994","DOI":"10.1128\/JB.186.15.4994-5002.2004","article-title":"Intraspecific phylogeny and lineage group identification based on the prfA virulence gene cluster of Listeria monocytogenes","volume":"186","author":"Ward","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0235","doi-asserted-by":"crossref","first-page":"7629","DOI":"10.1128\/AEM.01127-08","article-title":"Multilocus genotyping assays for single nucleotide polymorphism-based subtyping of Listeria monocytogenes isolates","volume":"74","author":"Ward","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0240","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.micres.2015.04.002","article-title":"Geographical and longitudinal analysis of Listeria monocytogenes genetic diversity reveals its correlation with virulence and unique evolution","volume":"175","author":"Yin","year":"2015","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0245","doi-asserted-by":"crossref","first-page":"5573","DOI":"10.1128\/JB.185.18.5573-5584.2003","article-title":"Genome diversification in phylogenetic lineages I and II of Listeria monocytogenes: identification of segments unique to lineage II populations","volume":"185","author":"Zhang","year":"2003","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ijfoodmicro.2019.108289_bb0250","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1017\/S0950268817000711","article-title":"Estimating the real incidence of invasive listeriosis through an integrated surveillance model in use in Lombardy (Italy, 2006\u20132014)","volume":"145","author":"Zolin","year":"2017","journal-title":"Epidemiol. Infect."}],"container-title":["International Journal of Food Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168160519302211?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0168160519302211?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T12:16:57Z","timestamp":1759148217000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0168160519302211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":52,"alternative-id":["S0168160519302211"],"URL":"https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2019.108289","relation":{},"ISSN":["0168-1605"],"issn-type":[{"value":"0168-1605","type":"print"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of Listeria monocytogenes serovar 1\/2a, 1\/2b, 1\/2c and 4b by high resolution melting analysis for epidemiological investigations","name":"articletitle","label":"Article Title"},{"value":"International Journal of Food Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2019.108289","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"108289"}}