{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T08:36:41Z","timestamp":1774600601291,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T00:00:00Z","timestamp":1347667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl J Gynecology &amp; Obste"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the concentration of syncytiotrophoblast\u2010derived microparticles (STBMs) in the maternal circulation and the rate of syncytiotrophoblast apoptosis in the placenta of patients with early\u2010onset and late\u2010onset severe pre\u2010eclampsia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective case\u2013control study was conducted that enrolled 15 women with early\u2010onset severe pre\u2010eclampsia, 15 women with late\u2010onset severe pre\u2010eclampsia, and 10 women with normal pregnancies. Plasma STBM levels were measured by enzyme\u2010linked immunosorbent assay, while placental levels of active caspase\u20103 were determined by western blotting. Human umbilical vein endothelial cells (HUVECs) were cultured with STBMs and the proliferation and apoptosis rates of the HUVECs assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Levels of STBMs in the early\u2010onset group (71.2 \u00b1 20.7 ng\/mL) were significantly higher than those detected in the late\u2010onset group (41.9 \u00b1 29.7 ng\/mL) and the control group (26.3 \u00b1 11.2 ng\/mL) (<jats:italic>P<\/jats:italic> &lt; 0.05). The amount of active caspase\u20103 was increased in the early\u2010onset (0.85 \u00b1 0.61) and late\u2010onset groups (0.77 \u00b1 0.46) relative to the control group (0.32 \u00b1 0.15) (<jats:italic>P<\/jats:italic> &lt; 0.05). Proliferation of HUVECs was inhibited, while apoptosis was elevated, following co\u2010culture with STBMs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Shedding of STBMs into the maternal circulation occurs in greater amounts in early\u2010onset pre\u2010eclampsia than in late\u2010onset pre\u2010eclampsia.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.ijgo.2012.07.010","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T04:37:08Z","timestamp":1347683828000},"page":"234-238","source":"Crossref","is-referenced-by-count":69,"title":["Syncytiotrophoblast\u2010derived microparticle shedding in early\u2010onset and late\u2010onset severe pre\u2010eclampsia"],"prefix":"10.1002","volume":"119","author":[{"given":"Yu","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajuan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongzhen","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yincheng","family":"Teng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,9,15]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60659-1"},{"key":"e_1_2_6_3_2","first-page":"69","article-title":"Endothelial dysfunction in pre\u2010eclampsia","volume":"58","author":"Poston L.","year":"2006","journal-title":"Pharmacol Rep"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2008.08.018"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.9.5949"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2010.10.011"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10641951003599571"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1270342"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.04.004"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-007-0425-2"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.01.014"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgyn.2007.08.003"},{"issue":"1","key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"IX","DOI":"10.3109\/10641950109152635","article-title":"The classification and diagnosis of the hypertensive disorders of pregnancy: statement from the International Society for the Study of Hypertension in Pregnancy (ISSHP)","volume":"20","author":"Brown M.A.","year":"2001","journal-title":"Hypertens Pregnancy"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.11.007"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1053\/plac.1998.0351"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107470"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.07.039"},{"issue":"2","key":"e_1_2_6_18_2","first-page":"159","article-title":"\u201cFalling leaves\u201d: a survey of the history of apoptosis","volume":"95","author":"Formigli L.","year":"2004","journal-title":"Minerva Med"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2008.04.009"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63857-6"},{"issue":"9","key":"e_1_2_6_21_2","first-page":"251","article-title":"Study on cell apoptosis in the placenta of preeclampsia","volume":"25","author":"Li J.","year":"2005","journal-title":"Reprod Contracept"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20729"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-004-0872-z"},{"issue":"1","key":"e_1_2_6_24_2","first-page":"G1","article-title":"Necrapoptosis and the mitochondrial permeability transition: shared pathways to necrosis and apoptosis","volume":"276","author":"Lemasters J.J.","year":"1999","journal-title":"Am J Physiol"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1602\/neurorx.1.1.5"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000196731.56062.7c"}],"container-title":["International Journal of Gynecology &amp; 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