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The specimens were analyzed by PCR\u2010directed sequencing of a 364\u2010bp fragment of the long control region of HPV\u201016, and a phylogenetic tree was compiled with MEGA 5.0 software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Most HPV\u201016 variants belonged to the European branch (82%); these variants were detected among 25 of 29 women with LSIL, 22 of 29 women with HSIL, 3 of 4 women with cervical cancer, and all women with normal cytology. Two isolates yielded new variants of the European branch with nucleotide substitutions at positions A7752C and A7810T. Non\u2010European variants, such as African type 1 (1.5%) and Asian\u2010American (16.5%), were detected only among women with cervical lesions (4\/29, LSIL; 6\/29, HSIL; 1\/29, cervical cancer). These variants had at least 6 nucleotide substitutions adjacent to or within transcription factor binding sites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>All branches of HPV\u201016 variants were detected among Paraguayan women with cervical lesions.<\/jats:p><\/jats:sec>","DOI":"10.1016\/j.ijgo.2013.01.020","type":"journal-article","created":{"date-parts":[[2013,3,16]],"date-time":"2013-03-16T07:15:42Z","timestamp":1363418142000},"page":"44-47","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Distribution of HPV\u201016 variants among isolates from Paraguayan women with different grades of cervical lesion"],"prefix":"10.1002","volume":"122","author":[{"given":"Laura","family":"Mendoza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda A.","family":"Picconi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Mirazo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Mongel\u00f3s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela","family":"Gim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Basiletti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Arbiza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,15]]},"reference":[{"issue":"12","key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1002\/ijc.25516","article-title":"Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008","volume":"127","author":"Ferlay J.","year":"2010","journal-title":"Int J Cancer"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021641"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/PDM.0b013e3181c1482c"},{"issue":"3","key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1128\/jvi.71.3.2463-2472.1997","article-title":"Human papillomavirus type 16 sequence variation in cervical cancers: a worldwide perspective","volume":"71","author":"Yamada T.","year":"1997","journal-title":"J Virol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00483-12"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-8-1975"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2010.01.036"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-4179"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2010.12.005"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.08.004"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.22112"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70158-5"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23373"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.16.2114"},{"issue":"11","key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"6413","DOI":"10.1128\/jvi.67.11.6413-6423.1993","article-title":"The genetic drift of human papillomavirus type 16 is a means of reconstructing prehistoric viral spread and the movement of ancient human populations","volume":"67","author":"Ho L.","year":"1993","journal-title":"J Virol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_6_18_2","unstructured":"MyersG. 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