{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T03:42:22Z","timestamp":1776397342369,"version":"3.51.2"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T00:00:00Z","timestamp":1377129600000},"content-version":"vor","delay-in-days":1086,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Infectious Diseases"],"published-print":{"date-parts":[[2010,9]]},"DOI":"10.1016\/j.ijid.2009.12.004","type":"journal-article","created":{"date-parts":[[2010,4,22]],"date-time":"2010-04-22T08:50:40Z","timestamp":1271926240000},"page":"e148-e153","source":"Crossref","is-referenced-by-count":12,"special_numbering":"S3","title":["First isolation of human Leptospira strains, Azores, Portugal"],"prefix":"10.1016","volume":"14","author":[{"given":"Ana T.","family":"Gon\u00e7alves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Paiva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Melo-Mota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lu\u00edsa","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Carreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica S.","family":"Nunes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00edsa","family":"Mota-Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudy A.","family":"Harstkeerl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karyne","family":"Hyde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarida","family":"Collares-Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijid.2009.12.004_bib1","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: a zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.ijid.2009.12.004_bib2","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1023\/A:1007383405833","article-title":"First epidemiological data on pathogenic leptospires isolated on the Azorean islands","volume":"13","author":"Collares-Pereira","year":"1997","journal-title":"Eur J Epidemiol"},{"key":"10.1016\/j.ijid.2009.12.004_bib3","series-title":"Leptospira and leptospirosis","author":"Faine","year":"1999"},{"key":"10.1016\/j.ijid.2009.12.004_bib4","unstructured":"Collares-Pereira M, Vieira ML. Fatal leptospirosis, Azores islands. Report submitted by the Institute of Hygiene and Tropical Medicine, Lisbon, Portugal. Wkly Epidemiol Rec 2001; 15:109\u201311."},{"key":"10.1016\/j.ijid.2009.12.004_bib5","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.ijid.2005.07.006","article-title":"Human leptospirosis in Portugal: a retrospective study of eighteen years","volume":"10","author":"Vieira","year":"2006","journal-title":"Int J Infect Dis"},{"key":"10.1016\/j.ijid.2009.12.004_bib6","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1023\/A:1010916132497","article-title":"Rodents and Leptospira transmission risk in Terceira island (Azores)","volume":"16","author":"Collares-Pereira","year":"2000","journal-title":"Eur J Epidemiol"},{"key":"10.1016\/j.ijid.2009.12.004_bib7","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1574-6968.2000.tb09059.x","article-title":"Analysis of Leptospira isolates from mainland Portugal and the Azores islands","volume":"185","author":"Collares-Pereira","year":"2000","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.ijid.2009.12.004_bib8","series-title":"Methods in microbiology","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0580-9517(08)70493-8","article-title":"Serological typing methods of leptospires","author":"Dikken","year":"1978"},{"key":"10.1016\/j.ijid.2009.12.004_bib9","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.ijid.2009.12.004_bib10","unstructured":"World Health Organization and the International Leptospirosis Society. Human leptospirosis: guidance for diagnosis, surveillance and control. Geneva, Switzerland: WHO; 2003."},{"key":"10.1016\/j.ijid.2009.12.004_bib11","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1099\/00222615-46-2-173","article-title":"Differentiation of Leptospira species and serovars by PCR-restriction endonuclease analysis, arbitrarily primed PCR and low-stringency PCR","volume":"46","author":"Brown","year":"1997","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.ijid.2009.12.004_bib12","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1128\/JCM.39.1.191-195.2001","article-title":"Identification of new repetitive elements in Leptospira interrogans serovar Copenhageni and its application to PCR-based differentiation of Leptospira serogroups","volume":"39","author":"Barochii","year":"2001","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijid.2009.12.004_bib13","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1476-0711-5-28","article-title":"Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species","volume":"5","author":"Ahmed","year":"2006","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"10.1016\/j.ijid.2009.12.004_bib14","doi-asserted-by":"crossref","first-page":"3510","DOI":"10.1128\/JCM.00670-06","article-title":"Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing","volume":"44","author":"Morey","year":"2006","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijid.2009.12.004_bib15","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1590\/S0074-02762006000400005","article-title":"Molecular characterization of Leptospira sp. strains isolated from human subjects in S\u00e3o Paulo, Brazil using a polymerase chain reaction-based assay: a public health tool","volume":"101","author":"Romero","year":"2006","journal-title":"Mem Inst Oswaldo Cruz."},{"key":"10.1016\/j.ijid.2009.12.004_bib16","unstructured":"Collares-Pereira M, Santos-Reis M, Gon\u00e7alves L, Vieira ML, Flor L. Epidemiology and control of leptospirosis in the Azores Project. Final scientific report (USA Scientific Cooperative Agreement No. 58-401-3-F185, 2003-2008); 2008. Available in part at: http:\/\/www.ars.usda.gov\/research\/projects\/projects.htm?ACCN_NO=406608&showpars=true&fy=2008.(accessed March 2010)."},{"key":"10.1016\/j.ijid.2009.12.004_bib17","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1128\/JCM.30.9.2219-2224.1992","article-title":"Polymerase chain reaction for detection of Leptospira spp. in clinical samples","volume":"30","author":"M\u00e9rien","year":"1992","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijid.2009.12.004_bib18","first-page":"27","article-title":"Differentiation of pathogenic and saprophytic leptospires","volume":"94","author":"Johnson","year":"1967","journal-title":"I. Growth at low temperatures. J Bacteriol"},{"key":"10.1016\/j.ijid.2009.12.004_bib19","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1128\/JB.88.6.1618-1623.1964","article-title":"Differentiation of pathogenic and saprophytic Leptospires with 8-azaguanine","volume":"88","author":"Johnson","year":"1964","journal-title":"J Bacteriol"},{"key":"10.1016\/j.ijid.2009.12.004_bib20","unstructured":"Johnson RC, Faine S. Order I. Spirochaetalis: Family II. Leptospiraceae Hovind-Hougen 1979. In: Krieg NR, Holt JG, eds. Bergey's manual of systematic bacteriology, 1st ed. Baltimore, MD, Williams & Wilkins, 1 (245): 62-67."},{"key":"10.1016\/j.ijid.2009.12.004_bib21","doi-asserted-by":"crossref","first-page":"e2752","DOI":"10.1371\/journal.pone.0002752","article-title":"Conservation of the S10-spc-\u03b1 locus within otherwise highly plastic genomes provides phylogenetic insight into the genus Leptospira","volume":"3","author":"Victoria","year":"2008","journal-title":"PLoS ONE"},{"key":"10.1016\/j.ijid.2009.12.004_bib22","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.ijid.2009.12.004_bib23","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1086\/346208","article-title":"Usefulness of serologic analysis as a predictor of the infecting serovar in patients with severe leptospirosis","volume":"36","author":"Levett","year":"2003","journal-title":"Clin Infect Dis"}],"container-title":["International Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971210023155?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971210023155?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T23:14:08Z","timestamp":1591226048000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1201971210023155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":23,"alternative-id":["S1201971210023155"],"URL":"https:\/\/doi.org\/10.1016\/j.ijid.2009.12.004","relation":{},"ISSN":["1201-9712"],"issn-type":[{"value":"1201-9712","type":"print"}],"subject":[],"published":{"date-parts":[[2010,9]]}}}