{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:34:33Z","timestamp":1792154073175,"version":"4.4.0"},"reference-count":10,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"DIRECCION DE INVESTIGACION E INNOVACION"},{"name":"DIRECCION ACADEMICA"}],"content-domain":{"domain":["ijidonline.com","clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Infectious Diseases"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1016\/j.ijid.2018.10.029","type":"journal-article","created":{"date-parts":[[2018,11,3]],"date-time":"2018-11-03T12:15:29Z","timestamp":1541247329000},"page":"1-3","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients"],"prefix":"10.1016","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-9312","authenticated-orcid":false,"given":"Juan David","family":"Ram\u00edrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanny","family":"Herrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Muskus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mendez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Clara","family":"Duque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Butcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijid.2018.10.029_bib0005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2016.11.012","article-title":"Leishmania infections: molecular targets and diagnosis","volume":"57","author":"Akhoundi","year":"2017","journal-title":"Mol Aspects Med"},{"issue":"5","key":"10.1016\/j.ijid.2018.10.029_bib0010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0035671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012","journal-title":"PLoS One"},{"issue":"1\u20132","key":"10.1016\/j.ijid.2018.10.029_bib0015","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s004300100085","article-title":"Worldwide increasing risk factors for leishmaniasis","volume":"190","author":"Desjeux","year":"2001","journal-title":"Med Microbiol Immunol"},{"issue":"2","key":"10.1016\/j.ijid.2018.10.029_bib0020","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1128\/JCM.02620-12","article-title":"Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus","volume":"51","author":"Hayden","year":"2013","journal-title":"J Clin Microbiol"},{"issue":"6","key":"10.1016\/j.ijid.2018.10.029_bib0025","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.1128\/JCM.00208-13","article-title":"A real-time PCR assay for detection and quantification of Leishmania (Viannia) in skin and mucosal lesions: an exploratory study of parasite load and clinical parameters","volume":"51","author":"Jara","year":"2013","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijid.2018.10.029_bib0030","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.3389\/fmicb.2017.01907","article-title":"Analytical performance of four polymerase chain reaction (PCR) and real time PCR (qPCR) assays for the detection of six leishmania species DNA in Colombia","volume":"8","author":"Le\u00f3n","year":"2017","journal-title":"Front Microbiol"},{"key":"10.1016\/j.ijid.2018.10.029_bib0035","series-title":"Analytical performance of a loop-mediated isothermal amplification assay for leishmania DNA detection in sandflies and direct smears of patients with cutaneous leishmaniasis","author":"Le\u00f3n","year":"2018"},{"issue":"4","key":"10.1016\/j.ijid.2018.10.029_bib0040","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1128\/JCM.43.4.1851-1857.2005","article-title":"Detection of human immunodeficiency virus type 1 DNA in dried blood spots by a duplex real-time PCR assay","volume":"43","author":"Luo","year":"2005","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijid.2018.10.029_bib0045","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.actatropica.2017.09.020","article-title":"The applicability of real-time PCR in the diagnostic of cutaneous leishmaniasis and parasite quantification for clinical management: current status and perspectives","volume":"184","author":"Moreira","year":"2018","journal-title":"Acta Trop"},{"issue":"1","key":"10.1016\/j.ijid.2018.10.029_bib0050","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s12977-018-0399-0","article-title":"Digital PCR as a tool to measure HIV persistence","volume":"15","author":"Rutsaert","year":"2018","journal-title":"Retrovirology"}],"container-title":["International Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971218345776?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971218345776?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T19:42:24Z","timestamp":1554493344000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1201971218345776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":10,"alternative-id":["S1201971218345776"],"URL":"https:\/\/doi.org\/10.1016\/j.ijid.2018.10.029","relation":{},"ISSN":["1201-9712"],"issn-type":[{"value":"1201-9712","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients","name":"articletitle","label":"Article Title"},{"value":"International Journal of Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijid.2018.10.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.","name":"copyright","label":"Copyright"}]}}