{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T19:49:14Z","timestamp":1785268154899,"version":"3.55.0"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ijidonline.com","clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Infectious Diseases"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1016\/j.ijid.2018.10.030","type":"journal-article","created":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T07:28:20Z","timestamp":1541489300000},"page":"116-122","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men"],"prefix":"10.1016","volume":"79","author":[{"given":"Joana","family":"Calado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngela","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jos\u00e9","family":"Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.ijid.2018.10.030_bib0005","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1128\/AAC.00788-10","article-title":"Molecular analysis of antimicrobial resistance mechanisms in Neisseria gonorrhoeae isolates from Ontario, Canada","volume":"55","author":"Allen","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"issue":"5","key":"10.1016\/j.ijid.2018.10.030_bib0010","doi-asserted-by":"crossref","first-page":"2528","DOI":"10.1128\/AAC.02608-13","article-title":"Azithromycin resistance is coevolving with reduced susceptibility to cephalosporins in Neisseria gonorrhoeae in Ontario, Canada","volume":"58","author":"Allen","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"issue":"12","key":"10.1016\/j.ijid.2018.10.030_bib0015","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1128\/AAC.46.12.3744-3749.2002","article-title":"Mosaic-like structure of penicillin binding protein 2 gene (penA) in clinical isolates of Neisseria gonorrhoeae with reduced susceptibility to cefixime","volume":"46","author":"Ameyama","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"10.1016\/j.ijid.2018.10.030_bib0020","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1136\/sti.2010.044164","article-title":"European surveillance of antimicrobial resistance in Neisseria gonorrhoeae","volume":"86","author":"Cole","year":"2010","journal-title":"Sex Transm Infect"},{"issue":"42","key":"10.1016\/j.ijid.2018.10.030_bib0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/ese.16.42.19995-en","article-title":"The European gonococcal antimicrobial surveillance programme, 2009","volume":"16","author":"Cole","year":"2011","journal-title":"Euro Surveill"},{"issue":"4","key":"10.1016\/j.ijid.2018.10.030_bib0030","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.bjm.2017.06.001","article-title":"Antimicrobial resistance in Neisseria gonorrhoeae: history, molecular mechanisms and epidemiological aspects of an emerging global threat","volume":"48","author":"Costa-Louren\u00e7o","year":"2017","journal-title":"Braz J Microbiol"},{"issue":"12","key":"10.1016\/j.ijid.2018.10.030_bib0035","doi-asserted-by":"crossref","first-page":"3877","DOI":"10.1128\/AAC.47.12.3877-3880.2003","article-title":"Acquired macrolide resistance genes in pathogenic Neisseria spp. isolated between 1940 and 1987","volume":"47","author":"Cousin","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.ijid.2018.10.030_bib0040","series-title":"Annual epidemiological report: reportingon 2011 surveillance data and 2012 epidemic intelligence data","author":"ECDC","year":"2013"},{"key":"10.1016\/j.ijid.2018.10.030_bib0045","series-title":"Gonococcal antimicrobial susceptibility surveillance in Europe 2015","author":"ECDC","year":"2017"},{"key":"10.1016\/j.ijid.2018.10.030_bib0050","unstructured":"EUCAST. Breakpoint tables for interpretation of MICs and zone diameters: version 4.0. 2014. Available from: http:\/\/www.eucast.org. [cited 2014 November 15]."},{"issue":"48","key":"10.1016\/j.ijid.2018.10.030_bib0055","doi-asserted-by":"crossref","first-page":"7596","DOI":"10.1021\/bi5011317","article-title":"Structural effect of the Asp345a insertion in penicillin-binding protein 2 from penicillin-resistant strains of Neisseria gonorrhoeae","volume":"53","author":"Fedarovich","year":"2014","journal-title":"Biochem"},{"issue":"6","key":"10.1016\/j.ijid.2018.10.030_bib0060","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1136\/sti.2010.044321","article-title":"Genotypes and antimicrobial-resistant phenotypes of Neisseria gonorrhoeae in Portugal (2004-2009)","volume":"86","author":"Florindo","year":"2010","journal-title":"Sex Transm Infect"},{"issue":"11","key":"10.1016\/j.ijid.2018.10.030_bib0065","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1086\/386312","article-title":"Quinolone resistance-determining region mutations and por type of Neisseria gonorrhoeae isolates: resistance surveillance and typing by molecular methodologies","volume":"189","author":"Giles","year":"2004","journal-title":"J Infect Dis"},{"issue":"November (11)","key":"10.1016\/j.ijid.2018.10.030_bib0070","doi-asserted-by":"crossref","first-page":"5633","DOI":"10.1128\/AAC.00636-12","article-title":"Genome sequencing of a Neisseria gonorrhoeae isolate of a successful international clone with decreased susceptibility and resistance to extended-spectrum cephalosporins","volume":"56","author":"Hess","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"10.1016\/j.ijid.2018.10.030_bib0075","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1128\/AAC.01420-07","article-title":"Relation between genetic markers of drug resistance and susceptibility profile of clinical Neisseria gonorrhoeae strains","volume":"52","author":"Ilina","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"issue":"3","key":"10.1016\/j.ijid.2018.10.030_bib0080","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1136\/sextrans-2012-050829","article-title":"Evolution of Neisseria gonorrhoeae is a continuing challenge for molecular detection of gonorrhoea: false negative gonococcal porA mutants are spreading internationally","volume":"89","author":"Ison","year":"2013","journal-title":"Sex Transm Infect"},{"issue":"1","key":"10.1016\/j.ijid.2018.10.030_bib0085","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1128\/AAC.49.1.137-143.2005","article-title":"Emergence and spread of Neisseria gonorrhoeae clinical isolates harboring mosaic-like structure of penicillin binding protein 2 in central Japan","volume":"49","author":"Ito","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"issue":"12","key":"10.1016\/j.ijid.2018.10.030_bib0090","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1093\/infdis\/jis277","article-title":"Impact of fluoroquinolone resistance mutations on gonococcal fitness and in vivo selection for compensatory mutations","volume":"205","author":"Kunz","year":"2012","journal-title":"J Infect Dis"},{"issue":"5","key":"10.1016\/j.ijid.2018.10.030_bib0095","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1093\/jac\/dkr021","article-title":"Analysis of mutations in multiple loci of Neisseria gonorrhoeae isolates reveals effects of PIB, PBP2 and MtrR on reduced susceptibility to ceftriaxone","volume":"66","author":"Liao","year":"2011","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.ijid.2018.10.030_bib0100","series-title":"Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world","author":"Perilla","year":"2003"},{"key":"10.1016\/j.ijid.2018.10.030_bib0105","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1111\/j.1469-0691.2008.02071.x","article-title":"Phenotypic and genotypic characterization of Neisseria gonorrhoeae in parts of Italy: detection of a multiresistant cluster circulating in a heterosexual network","volume":"14","author":"Starnino","year":"2008","journal-title":"Clin Microbiol Infect"},{"issue":"2","key":"10.1016\/j.ijid.2018.10.030_bib0110","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1128\/JCM.38.2.521-525.2000","article-title":"Antimicrobial resistance of Neisseria gonorrhoeae and high prevalence of ciprofloxacin-resistant isolates in Japan, 1993 to 1998","volume":"38","author":"Tanaka","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"7","key":"10.1016\/j.ijid.2018.10.030_bib0115","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1586\/eri.09.63","article-title":"Meeting the public health challenge of multidrug and extensively drug resistant Neisseria gonorrhoeae","volume":"7","author":"Tapsall","year":"2009","journal-title":"Expert Rev Anti Infect Ther"},{"key":"10.1016\/j.ijid.2018.10.030_bib0120","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1099\/jmm.0.074286-0","article-title":"A Neisseria gonorrhoeae strain with a meningococcal mtrR sequence","volume":"63","author":"Trembizki","year":"2014","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.ijid.2018.10.030_bib0125","series-title":"Laboratory diagnosis of sexually transmitted infections, including human immunodeficiency virus","author":"Unemo","year":"2013"},{"issue":"12","key":"10.1016\/j.ijid.2018.10.030_bib0130","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.2217\/fmb.12.117","article-title":"Emergence of multidrug-resistant, extensively drug- resistant and untreatable gonorrhea","volume":"7","author":"Unemo","year":"2012","journal-title":"Futur Microbiol"},{"issue":"3","key":"10.1016\/j.ijid.2018.10.030_bib0135","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1128\/CMR.00010-14","article-title":"Antimicrobial resistance in Neisseria gonorrhoeae in the 21st Century: past, evolution, and future","volume":"27","author":"Unemo","year":"2014","journal-title":"Clin Microbiol Rev"},{"issue":"20","key":"10.1016\/j.ijid.2018.10.030_bib0140","doi-asserted-by":"crossref","first-page":"5619","DOI":"10.1128\/JB.184.20.5619-5624.2002","article-title":"Overexpression of the MtrC-MtrD-MtrE efflux pump due to an mtrR mutation is required for chromosomally mediated penicillin resistance in Neisseria gonorrhoeae","volume":"184","author":"Veal","year":"2002","journal-title":"J Bacteriol"},{"issue":"2","key":"10.1016\/j.ijid.2018.10.030_bib0145","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1111\/j.1365-2958.2008.06424.x","article-title":"Clinically relevant mutations that cause derepression of the Neisseria gonorrhoeae MtrC-MtrD-MtrE efflux pump system confer different levels of antimicrobial resistance and in vivo fitness","volume":"70","author":"Warner","year":"2008","journal-title":"Mol Microbiol"},{"issue":"9","key":"10.1016\/j.ijid.2018.10.030_bib0150","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1128\/AAC.00306-07","article-title":"Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone","volume":"51","author":"Whiley","year":"2007","journal-title":"Antimicrob Agents Chemother"}],"container-title":["International Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971218345788?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971218345788?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,9,5]],"date-time":"2022-09-05T10:46:29Z","timestamp":1662374789000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1201971218345788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":30,"alternative-id":["S1201971218345788"],"URL":"https:\/\/doi.org\/10.1016\/j.ijid.2018.10.030","relation":{},"ISSN":["1201-9712"],"issn-type":[{"value":"1201-9712","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Antimicrobial resistance and molecular characteristics of Neisseria gonorrhoeae isolates from men who have sex with men","name":"articletitle","label":"Article Title"},{"value":"International Journal of Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijid.2018.10.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.","name":"copyright","label":"Copyright"}]}}