{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T22:07:37Z","timestamp":1782338857549,"version":"3.54.5"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T00:00:00Z","timestamp":1640822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","ijidonline.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Infectious Diseases"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.ijid.2021.12.361","type":"journal-article","created":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T12:16:44Z","timestamp":1641212204000},"page":"34-37","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Development, verification, and validation of an RT-qPCR-based protocol for Yellow Fever diagnosis"],"prefix":"10.1016","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8367-999X","authenticated-orcid":false,"given":"Rita de C\u00e1ssia Pontello","family":"Rampazzo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam Ribas","family":"Zambenedetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Alexandrino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1348-1523","authenticated-orcid":false,"given":"Thiago","family":"Jacomasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel Kruchelski","family":"Tsch\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria Bispo","family":"de Fillipis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Gustavo","family":"Morello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5695-2746","authenticated-orcid":false,"given":"Fabricio Klerynton","family":"Marchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijid.2021.12.361_bib0001","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jviromet.2019.03.014","article-title":"Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus","volume":"268","author":"Boga","year":"2019","journal-title":"J Virol Methods"},{"key":"10.1016\/j.ijid.2021.12.361_bib0002","author":"Borghetti","year":"2019","journal-title":"External Control Viral-Like Particle Construction for Detection of Emergent Arboviruses by Real-Time Reverse-Transcription PCR"},{"key":"10.1016\/j.ijid.2021.12.361_bib0003","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1743-422X-9-27","article-title":"Development of a SYBR green I based RT-PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti","volume":"9","author":"Dash","year":"2012","journal-title":"Virol J"},{"key":"10.1016\/j.ijid.2021.12.361_bib0004","doi-asserted-by":"crossref","DOI":"10.1038\/s41426-018-0128-8","article-title":"Yellow fever in the diagnostics laboratory review-article","volume":"7","author":"Domingo","year":"2018","journal-title":"Emerg Microbes Infect"},{"key":"10.1016\/j.ijid.2021.12.361_bib0005","first-page":"4054","volume":"50","author":"Domingo","year":"2012","journal-title":"Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories"},{"key":"10.1016\/j.ijid.2021.12.361_bib0006","first-page":"2323","volume":"40","author":"Drosten","year":"2002","journal-title":"Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR"},{"key":"10.1016\/j.ijid.2021.12.361_bib0007","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002730","article-title":"Rapid Molecular Assays for the Detection of Yellow Fever Virus in Low-Resource Settings","volume":"8","author":"Escadafal","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.ijid.2021.12.361_bib0008","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.cll.2010.01.001","article-title":"Yellow fever: a reemerging threat. (Special Issue: Emerging pathogens.)","volume":"30","author":"Gardner","year":"2010","journal-title":"Clin Lab Med"},{"key":"10.1016\/j.ijid.2021.12.361_bib0009","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.bjid.2017.02.004","article-title":"Yellow fever outbreak in Brazil, 2017","volume":"21","author":"Goldani","year":"2017","journal-title":"Brazilian J Infect Dis"},{"issue":"1","key":"10.1016\/j.ijid.2021.12.361_bib0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JVI.01623-19","article-title":"Yellow Fever Virus Reemergence and Spread in Southeast Brazil, 2016-2019","volume":"94","author":"Giovanetti","year":"2019","journal-title":"J Virol"},{"key":"10.1016\/j.ijid.2021.12.361_bib0011","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.coviro.2019.01.001","article-title":"Emergence and re-emergence of mosquito-borne arboviruses","volume":"34","author":"Huang","year":"2019","journal-title":"Curr Opin Virol"},{"key":"10.1016\/j.ijid.2021.12.361_bib0012","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The Measurement of Observer Agreement for Categorical Data","volume":"33","author":"Landis","year":"1977","journal-title":"Biometrics"},{"key":"10.1016\/j.ijid.2021.12.361_bib0013","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0008325","article-title":"Ultra-sensitive detection of mycobacterium leprae: DNA extraction and PCR assays","volume":"14","author":"de Manta","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.ijid.2021.12.361_bib0014","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/B978-012374410-4.00535-5","article-title":"Yellow Fever Virus","author":"Marfin","year":"2008","journal-title":"Encycl Virol"},{"key":"10.1016\/j.ijid.2021.12.361_bib0015","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jcv.2014.08.030","article-title":"Yellow fever","volume":"64","author":"Monath","year":"2015","journal-title":"J Clin Virol"},{"key":"10.1016\/j.ijid.2021.12.361_bib0016","volume":"51","year":"2020","journal-title":"Monitoramento dos casos de arboviroses urbanas transmitidas pelo Aedes"},{"key":"10.1016\/j.ijid.2021.12.361_bib0017","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jviromet.2011.02.025","article-title":"Evaluation of two molecular methods for the detection of Yellow fever virus genome","volume":"174","author":"Nunes","year":"2011","journal-title":"J Virol Methods"},{"key":"10.1016\/j.ijid.2021.12.361_bib0018","doi-asserted-by":"crossref","DOI":"10.1590\/0074-02760180278","article-title":"Yellow fever outbreak in Brazil: the puzzle of rapid viral spread and challenges for immunisation","volume":"113","author":"Possas","year":"2018","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.ijid.2021.12.361_bib0020","doi-asserted-by":"crossref","unstructured":"Rojas A, Diagne CT, Stittleburg VD, Mohamed-hadley A, Ar Y, Balmaseda A, et\u00a0al. Internally Controlled, Multiplex Real-Time Reverse Transcription PCR for Dengue Virus and Yellow Fever Virus Detection 2018;98:1833\u20136. https:\/\/doi.org\/10.4269\/ajtmh.18-0024.","DOI":"10.4269\/ajtmh.18-0024"},{"key":"10.1016\/j.ijid.2021.12.361_bib0019","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.vetpar.2017.09.009","article-title":"A ready-to-use duplex qPCR to detect Leishmania infantum DNA in naturally infected dogs","volume":"246","author":"Rampazzo","year":"2017","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.ijid.2021.12.361_bib0021","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1371\/journal.pntd.0002740","article-title":"Yellow Fever Outbreaks in Unvaccinated Populations, Brazil, 2008-2009","volume":"8","author":"Romano","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.ijid.2021.12.361_bib0022","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JCM.00827-18","article-title":"Yellow fever virus: Diagnostics for a persistent arboviral threat","volume":"56","author":"Waggoner","year":"2018","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijid.2021.12.361_bib0023","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jcv.2010.04.013","article-title":"Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains","volume":"48","author":"Weidmann","year":"2010","journal-title":"J Clin Virol"},{"key":"10.1016\/j.ijid.2021.12.361_bib0024","doi-asserted-by":"crossref","first-page":"e101","DOI":"10.1016\/S1473-3099(16)30518-7","article-title":"Epidemic arboviral diseases: priorities for research and public health","volume":"17","author":"Wilder-Smith","year":"2017","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.ijid.2021.12.361_bib0025","unstructured":"World Health Organization. (2019, May 7). \"Yellow fever\" access in https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/yellow-fever."},{"key":"10.1016\/j.ijid.2021.12.361_bib0027","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1590\/0074-02760160380","article-title":"Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics","volume":"112","author":"Zambenedetti","year":"2017","journal-title":"Mem Inst Oswaldo Cruz"}],"container-title":["International Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971221012571?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971221012571?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T08:06:17Z","timestamp":1761120377000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1201971221012571"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":26,"alternative-id":["S1201971221012571"],"URL":"https:\/\/doi.org\/10.1016\/j.ijid.2021.12.361","relation":{},"ISSN":["1201-9712"],"issn-type":[{"value":"1201-9712","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development, verification, and validation of an RT-qPCR-based protocol for Yellow Fever diagnosis","name":"articletitle","label":"Article Title"},{"value":"International Journal of Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijid.2021.12.361","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.","name":"copyright","label":"Copyright"}]}}