{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T05:52:38Z","timestamp":1774417958195,"version":"3.50.1"},"reference-count":20,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","ijidonline.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Infectious Diseases"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1016\/j.ijid.2026.108454","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:11:12Z","timestamp":1770077472000},"page":"108454","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A case of Human Pegivirus acute febrile infection diagnosed by Next Generation Sequencing (NGS)"],"prefix":"10.1016","volume":"165","author":[{"given":"Anna","family":"Anselmo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"D\u2019Ugo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Corrent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Giuseppetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossella","family":"Brandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Bucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Gioacchini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Sonnessa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Baggieri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Fillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AnnaTeresa","family":"Palamara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florigio","family":"Lista","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0394-7043","authenticated-orcid":false,"given":"Fabio","family":"Magurano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijid.2026.108454_bib0001","doi-asserted-by":"crossref","first-page":"ftae006","DOI":"10.1093\/femspd\/ftae006","article-title":"From fever to action: diagnosis, treatment, and prevention of acute undifferentiated febrile illnesses","volume":"82","author":"Barathan","year":"2024","journal-title":"Pathog Dis"},{"key":"10.1016\/j.ijid.2026.108454_bib0002","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/gm485","article-title":"Metagenomics for pathogen detection in public health","volume":"5","author":"Miller","year":"2013","journal-title":"Genome Med"},{"issue":"4","key":"10.1016\/j.ijid.2026.108454_bib0003","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.jinf.2019.08.003","article-title":"Metagenomic next-generation sequencing aids the diagnosis of viral infections in febrile returning travelers","volume":"79","author":"Jerome","year":"2019","journal-title":"J Infect"},{"key":"10.1016\/j.ijid.2026.108454_bib0004","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2021.104940","article-title":"Viral metagenomic sequencing in a cohort of international travelers returning with febrile illness","volume":"143","author":"Reyes","year":"2021","journal-title":"J Clin Virol"},{"issue":"1","key":"10.1016\/j.ijid.2026.108454_bib0005","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/nrg.2017.88","article-title":"Towards a genomics-informed, real-time, global pathogen surveillance system","volume":"19","author":"Gardy","year":"2018","journal-title":"Nat Rev Genet"},{"key":"10.1016\/j.ijid.2026.108454_bib0006","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jbiotec.2016.12.022","article-title":"Application of next generation sequencing in clinical microbiology and infection prevention","volume":"243","author":"Deurenberg","year":"2017","journal-title":"J Biotechnol"},{"issue":"50","key":"10.1016\/j.ijid.2026.108454_bib0007","first-page":"e2536","article-title":"Using a pan-viral microarray assay (Virochip) to screen clinical samples for viral pathogens","author":"Chen","year":"2011","journal-title":"J Vis Exp"},{"issue":"23","key":"10.1016\/j.ijid.2026.108454_bib0008","doi-asserted-by":"crossref","first-page":"3740","DOI":"10.1093\/bioinformatics\/btx520","article-title":"MetaCache: context-aware classification of metagenomic reads using minhashing","volume":"33","author":"M\u00fcller","year":"2017","journal-title":"Bioinformatics"},{"issue":"5","key":"10.1016\/j.ijid.2026.108454_bib0009","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1101\/gr.215087.116","article-title":"Canu: scalable and accurate long-read assembly via adaptive k-Mer weighting and repeat separation","volume":"27","author":"Koren","year":"2017","journal-title":"Genome Res"},{"key":"10.1016\/j.ijid.2026.108454_bib0010","doi-asserted-by":"crossref","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA 11: molecular evolutionary genetics analysis version 11","author":"Tamura","year":"2021","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.ijid.2026.108454_bib0011","doi-asserted-by":"crossref","first-page":"3.13.1","DOI":"10.1002\/0471250953.bi0313s48","article-title":"Clustal omega","volume":"48","author":"Sievers","year":"2014","journal-title":"Curr Protoc Bioinformatics"},{"key":"10.1016\/j.ijid.2026.108454_bib0012","unstructured":"Andrews, S (2010). FastQC: a quality control tool for high throughput sequence data. http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"10.1016\/j.ijid.2026.108454_bib0013","doi-asserted-by":"crossref","first-page":"e107","DOI":"10.1002\/imt2.107","article-title":"Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp","volume":"2","author":"Chen","year":"2023","journal-title":"Imeta"},{"key":"10.1016\/j.ijid.2026.108454_bib0014","doi-asserted-by":"crossref","first-page":"2815","DOI":"10.1038\/s41596-022-00738-y","article-title":"Metagenome analysis using the Kraken software suite","volume":"17","author":"Lu","year":"2022","journal-title":"Nat Protoc"},{"issue":"1","key":"10.1016\/j.ijid.2026.108454_bib0015","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/microorganisms12010019","article-title":"Pegivirus detection in cerebrospinal fluid from patients with central nervous system infections of unknown etiology in Brazil by viral metagenomics","volume":"12","author":"Carmona","year":"2024","journal-title":"Microorganisms"},{"key":"10.1016\/j.ijid.2026.108454_bib0016","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/vox.12876","article-title":"Global prevalence of human pegivirus-1 in healthy volunteer blood donors: a systematic review and meta-analysis","volume":"115","author":"Yang","year":"2020","journal-title":"Vox Sang"},{"key":"10.1016\/j.ijid.2026.108454_bib0017","doi-asserted-by":"crossref","first-page":"601","DOI":"10.3390\/pathogens14060601","article-title":"Deploying metagenomics to characterize microbial pathogens during outbreak of acute febrile illness among children in Tanzania","volume":"14","author":"Mziray","year":"2025","journal-title":"Pathogens"},{"issue":"15_supplement","key":"10.1016\/j.ijid.2026.108454_bib0018","doi-asserted-by":"crossref","first-page":"4656","DOI":"10.1212\/WNL.94.15_supplement.4656","article-title":"Human pegivirus-1 is neurotropic and associated with neuroinflammation","volume":"94","author":"Doan","year":"2020","journal-title":"Neurology"},{"issue":"1","key":"10.1016\/j.ijid.2026.108454_bib0019","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s13000-025-01664-9","article-title":"Human pegivirus detected in patient with reversible severe encephalitis and axillary lymphadenopathy: a case report","volume":"20","author":"He","year":"2025","journal-title":"Diagn Pathol"},{"key":"10.1016\/j.ijid.2026.108454_bib0020","doi-asserted-by":"crossref","first-page":"922","DOI":"10.3390\/genes15070922","article-title":"Exploring microorganisms associated to acute febrile illness and severe neurological disorders of unknown origin: a nanopore metagenomics approach","volume":"15","author":"Moreno","year":"2024","journal-title":"Genes"}],"container-title":["International Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971226000895?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1201971226000895?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T04:56:36Z","timestamp":1774414596000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1201971226000895"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":20,"alternative-id":["S1201971226000895"],"URL":"https:\/\/doi.org\/10.1016\/j.ijid.2026.108454","relation":{},"ISSN":["1201-9712"],"issn-type":[{"value":"1201-9712","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A case of Human Pegivirus acute febrile infection diagnosed by Next Generation Sequencing (NGS)","name":"articletitle","label":"Article Title"},{"value":"International Journal of Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijid.2026.108454","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.","name":"copyright","label":"Copyright"}],"article-number":"108454"}}