{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T17:00:16Z","timestamp":1761411616721,"version":"build-2065373602"},"reference-count":51,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI1204"],"award-info":[{"award-number":["01KI1204"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Medical Microbiology"],"published-print":{"date-parts":[[2015,5]]},"DOI":"10.1016\/j.ijmm.2015.02.005","type":"journal-article","created":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T12:30:15Z","timestamp":1424435415000},"page":"370-377","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Direct RNA-based detection of CTX-M \u03b2-lactamases in human blood samples"],"prefix":"10.1016","volume":"305","author":[{"given":"Claudia","family":"Stein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliwia","family":"Makarewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Pfeifer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Brandt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias W.","family":"Pletz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijmm.2015.02.005_bib0005","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1186\/cc11202","article-title":"Bench-to-bedside review: rapid molecular diagnostics for bloodstream infection\u2014a new frontier?","volume":"16","author":"Afshari","year":"2012","journal-title":"Crit. Care"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0010","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1164\/rccm.200405-644ST","article-title":"Guidelines for the management of adults with hospital-acquired, ventilator-associated, and healthcare-associated pneumonia","volume":"171","author":"ATS\/IDSA","year":"2005","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0015","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.drup.2006.05.005","article-title":"What's new in antibiotic resistance? Focus on beta-lactamases","volume":"9","author":"Babic","year":"2006","journal-title":"Drug Resist. Updates"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0020","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/j.1469-0691.2012.03821.x","article-title":"Rapid evolution and spread of carbapenemases among Enterobacteriaceae in Europe","volume":"18","author":"Canton","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0025","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.mib.2006.08.011","article-title":"The CTX-M beta-lactamase pandemic","volume":"9","author":"Canton","year":"2006","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0030","doi-asserted-by":"crossref","first-page":"110","DOI":"10.3389\/fmicb.2012.00110","article-title":"CTX-M enzymes: origin and diffusion","volume":"3","author":"Canton","year":"2012","journal-title":"Front. Microbiol."},{"issue":"Suppl. 1","key":"10.1016\/j.ijmm.2015.02.005_bib0035","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1111\/j.1469-0691.2007.01850.x","article-title":"Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe","volume":"14","author":"Canton","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0040","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0045","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1093\/jac\/dks156","article-title":"Evaluation of a DNA microarray for the rapid detection of extended-spectrum -lactamases (TEM, SHV and CTX-M), plasmid-mediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT\/MIR and CMY-1-like\/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM)","volume":"67","author":"Cuzon","year":"2012","journal-title":"J. Antimicrob. Chemoth."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0050","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1055\/s-0032-1325924","article-title":"Epidemiologie, Diagnostik und Therapie erwachsener Patienten mit nosokomialer Pneumonie","volume":"66","author":"Dalhoff","year":"2012","journal-title":"Pneumologie"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0055","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.mimet.2013.02.005","article-title":"Time-to-positivity-based discrimination between Enterobacteriaceae, Pseudomonas aeruginosa and strictly anaerobic Gram-negative bacilli in aerobic and anaerobic blood culture vials","volume":"93","author":"Defrance","year":"2013","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0060","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00134-007-0934-2","article-title":"Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008","volume":"34","author":"Dellinger","year":"2008","journal-title":"Intensive Care Med."},{"issue":"Suppl. 1","key":"10.1016\/j.ijmm.2015.02.005_bib0065","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1469-0691.2007.01846.x","article-title":"Phenotypic detection of extended-spectrum beta-lactamase production in Enterobacteriaceae: review and bench guide","volume":"14","author":"Drieux","year":"2008","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0070","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/1471-2334-12-116","article-title":"Increasing prevalence of ESBL production among Irish clinical Enterobacteriaceae from 2004 to 2008: an observational study","volume":"12","author":"Fennell","year":"2012","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0075","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/2110-5820-3-18","article-title":"Outbreak of multidrug-resistant Klebsiella pneumoniae carrying qnrB1 and blaCTX-M15 in a French intensive care unit","volume":"3","author":"Filippa","year":"2013","journal-title":"Ann. Intensive Care"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0080","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.1128\/JCM.01023-12","article-title":"Detection of extended-spectrum beta-lactamase and Klebsiella pneumoniae Carbapenemase genes directly from blood cultures by use of a nucleic acid microarray","volume":"50","author":"Fishbain","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0085","doi-asserted-by":"crossref","first-page":"e371","DOI":"10.1016\/j.ijid.2012.01.008","article-title":"Bloodstream infection with extended-spectrum beta-lactamase-producing Enterobacteriaceae at a tertiary care hospital in New Zealand: risk factors and outcomes","volume":"16","author":"Freeman","year":"2012","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0090","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1128\/JCM.01976-10","article-title":"Rapid identification of gram-negative bacteria with and without CTX-M extended-spectrum beta-lactamase from positive blood culture bottles by PCR followed by microchip gel electrophoresis","volume":"49","author":"Fujita","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0095","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1128\/JCM.02130-10","article-title":"Comparison of nine phenotypic methods for detection of extended-spectrum beta-lactamase production by Enterobacteriaceae","volume":"49","author":"Garrec","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0100","first-page":"1689","article-title":"Excess mortality and prolongation of stay due to bloodstream infections caused by multiresistant pathogens in Germany","volume":"137","author":"Gastmeier","year":"2012","journal-title":"Dtsch. Med. Wochenschr."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0105","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1128\/JCM.06852-11","article-title":"Current trends in culture-based and molecular detection of extended-spectrum-beta-lactamase-harboring and carbapenem-resistant Enterobacteriaceae","volume":"50","author":"Gazin","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0110","first-page":"301","article-title":"Antibacterial effects of water-soluble low-molecular-weight chitosans on different microorganisms","volume":"40","author":"Gerasimenko","year":"2004","journal-title":"Prikl. Biokhim. Mikrobiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0115","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1001\/archinte.165.12.1375","article-title":"Impact of inadequate initial antimicrobial therapy on mortality in infections due to extended-spectrum beta-lactamase-producing enterobacteriaceae: variability by site of infection","volume":"165","author":"Hyle","year":"2005","journal-title":"Arch. Intern. Med."},{"key":"10.1016\/j.ijmm.2015.02.005_bib1120","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1093\/jac\/dkm256","article-title":"Rapid detection of extended-spectrum beta-lactamase-producing Gram-negative bacilli in blood cultures","volume":"60","author":"Jain","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0120","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1128\/jcm.20.4.618-623.1984","article-title":"Clinical laboratory comparison of the 10-ml isolator blood culture system with BACTEC radiometric blood culture media","volume":"20","author":"Kellogg","year":"1984","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0130","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1128\/JCM.06115-11","article-title":"Using nucleic acid microarrays to perform molecular epidemiology and detect novel beta-lactamases: a snapshot of extended-spectrum beta-lactamases throughout the world","volume":"50","author":"Lascols","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0135","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1469-0691.2011.03703.x","article-title":"EUCAST expert rules in antimicrobial susceptibility testing","volume":"19","author":"Leclercq","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0140","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3389\/fmicb.2013.00080","article-title":"Commensal Enterobacteriaceae as reservoirs of extended-spectrum beta-lactamases, integrons, and sul genes in Portugal","volume":"4","author":"Machado","year":"2013","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0145","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/1471-2334-6-24","article-title":"Nosocomial bloodstream infections caused by Klebsiella pneumoniae: impact of extended-spectrum beta-lactamase (ESBL) production on clinical outcome in a hospital with high ESBL prevalence","volume":"6","author":"Marra","year":"2006","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0150","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1159\/000339483","article-title":"Risk factors associated with extended-spectrum beta-lactamase-producing Enterobacteriaceae nosocomial bloodstream infections in a tertiary care hospital: a clinical and molecular analysis","volume":"58","author":"Muro","year":"2012","journal-title":"Chemotherapy"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0155","doi-asserted-by":"crossref","first-page":"3086","DOI":"10.1128\/AAC.01298-09","article-title":"Evaluation of a DNA microarray, the check-points ESBL\/KPC array, for rapid detection of TEM, SHV, and CTX-M extended-spectrum beta-lactamases and KPC carbapenemases","volume":"54","author":"Naas","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0160","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3201\/eid1710.110655","article-title":"Global spread of Carbapenemase-producing Enterobacteriaceae","volume":"17","author":"Nordmann","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0165","doi-asserted-by":"crossref","first-page":"3829","DOI":"10.1128\/AAC.46.12.3829-3836.2002","article-title":"Mechanisms of decreased susceptibility to cefpodoxime in Escherichia coli","volume":"46","author":"Oliver","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0170","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.3201\/eid1707.110209","article-title":"Extended-spectrum beta-lactamase genes of Escherichia coli in chicken meat and humans, The Netherlands","volume":"17","author":"Overdevest","year":"2011","journal-title":"Emerg. Infect. Dis."},{"issue":"Suppl. 2","key":"10.1016\/j.ijmm.2015.02.005_bib0175","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1016\/j.ijantimicag.2010.11.010","article-title":"Conventional and molecular techniques for the early diagnosis of bacteraemia","volume":"36","author":"Paolucci","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0180","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1007\/s00134-010-1864-y","article-title":"Pharmacokinetics of moxifloxacin in patients with severe sepsis or septic shock","volume":"36","author":"Pletz","year":"2010","journal-title":"Intensive Care Med."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0185","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.leukres.2005.08.027","article-title":"Assessment of a new RNA stabilizing reagent (Tempus Blood RNA) for minimal residual disease in onco-hematology using the EAC protocol","volume":"30","author":"Prezeau","year":"2006","journal-title":"Leuk. Res."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0190","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1086\/521260","article-title":"Screening for extended-spectrum beta-lactamase-producing Enterobacteriaceae among high-risk patients and rates of subsequent bacteremia","volume":"45","author":"Reddy","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0195","first-page":"Doc14","volume":"8","author":"Reinhart","year":"2010","journal-title":"Ger. Med. Sci."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0200","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1111\/j.1600-0463.2012.02879.x","article-title":"First outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a Norwegian neonatal intensive care unit; associated with contaminated breast milk and resolved by strict cohorting","volume":"120","author":"Rettedal","year":"2012","journal-title":"Apmis"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0205","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1086\/508877","article-title":"Bacteremia due to extended-spectrum beta-lactamase-producing Escherichia coli in the CTX-M era: a new clinical challenge","volume":"43","author":"Rodriguez-Bano","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0210","series-title":"PCR Topics. Usage of PCR in Genetic and Infectious Diseases","first-page":"1","article-title":"PCR principles and reaction components","author":"Rolfs","year":"1991"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0215","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1471-2334-11-12","article-title":"Clinical profiles of patients colonized or infected with extended-spectrum beta-lactamase producing Enterobacteriaceae isolates: a 20 month retrospective study at a Belgian University Hospital","volume":"11","author":"Schoevaerdts","year":"2011","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0220","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1002\/prot.340020405","article-title":"Stability of wild-type and mutant RTEM-1 beta-lactamases: effect of the disulfide bond","volume":"2","author":"Schultz","year":"1987","journal-title":"Proteins"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0225","doi-asserted-by":"crossref","first-page":"992","DOI":"10.3346\/jkms.2010.25.7.992","article-title":"Bloodstream infections and clinical significance of healthcare-associated bacteremia: a multicenter surveillance study in Korean hospitals","volume":"25","author":"Son","year":"2010","journal-title":"J. Korean Med. Sci."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0230","doi-asserted-by":"crossref","first-page":"e80079","DOI":"10.1371\/journal.pone.0080079","article-title":"Direct RNA-based detection and differentiation of CTX-M-type extended-spectrum beta-lactamases (ESBL)","volume":"8","author":"Stein","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.ijmm.2015.02.005_bib0235","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1128\/AAC.01509-06","article-title":"Predictors of mortality in patients with bloodstream infections caused by extended-spectrum-beta-lactamase-producing Enterobacteriaceae: importance of inadequate initial antimicrobial treatment","volume":"51","author":"Tumbarello","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0240","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1128\/AAC.50.2.498-504.2006","article-title":"Bloodstream infections caused by extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae: risk factors, molecular epidemiology, and clinical outcome","volume":"50","author":"Tumbarello","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0245","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijmm.2014.07.015","article-title":"Subgrouping of ESBL-producing Escherichia coli from animal and human sources: An approach to quantify the distribution of ESBL types between different reservoirs","author":"Valentin","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0250","first-page":"121","article-title":"A multicenter cohort study on colonization and infection with extended-spectrum beta-lactamase producing Enterobacteriaceae (ESBL-E) in high-risk patients with hematological malignancies","volume":"37","author":"Vehreschild","year":"2014","journal-title":"Oncol. Res. Treat."},{"key":"10.1016\/j.ijmm.2015.02.005_bib0255","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/CMR.3.3.269","article-title":"Quantitative aspects of septicemia","volume":"3","author":"Yagupsky","year":"1990","journal-title":"Clin. Microbiol. Rev."}],"container-title":["International Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422115000168?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422115000168?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T16:57:17Z","timestamp":1761411437000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1438422115000168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["S1438422115000168"],"URL":"https:\/\/doi.org\/10.1016\/j.ijmm.2015.02.005","relation":{},"ISSN":["1438-4221"],"issn-type":[{"type":"print","value":"1438-4221"}],"subject":[],"published":{"date-parts":[[2015,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Direct RNA-based detection of CTX-M \u03b2-lactamases in human blood samples","name":"articletitle","label":"Article Title"},{"value":"International Journal of Medical Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijmm.2015.02.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}