{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:15:48Z","timestamp":1792631748155,"version":"4.5.0"},"reference-count":47,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100008924","name":"University of North Florida","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Medical Microbiology"],"published-print":{"date-parts":[[2019,5]]},"DOI":"10.1016\/j.ijmm.2019.04.001","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T11:52:03Z","timestamp":1555415523000},"page":"213-224","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"title":["Porin loss in Klebsiella pneumoniae clinical isolates impacts production of virulence factors and survival within macrophages"],"prefix":"10.1016","volume":"309","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2346-4652","authenticated-orcid":false,"given":"Debra N.","family":"Brunson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emir","family":"Maldosevic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Velez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Figgins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri N.","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijmm.2019.04.001_bib0005","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1128\/iai.61.3.852-860.1993","article-title":"C1q binding and activation of the complement classical pathway by Klebsiella pneumoniae outer membrane proteins","volume":"61","author":"Alberti","year":"1993","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0010","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1128\/iai.63.3.903-910.1995","article-title":"A porin from Klebsiella pneumoniae: sequence homology, three-dimensional model, and complement binding","volume":"63","author":"Alberti","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0015","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1128\/AAC.42.7.1636","article-title":"Outer membrane profiles of clonally related Klebsiella pneumoniae isolates from clinical samples and activities of cephalosporins and carbapenems","volume":"42","author":"Ardanuy","year":"1998","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0020","doi-asserted-by":"crossref","first-page":"64","DOI":"10.12703\/P6-64","article-title":"Klebsiella: a long way to go towards understanding this enigmatic jet-setter","volume":"6","author":"Broberg","year":"2014","journal-title":"F1000Prime Rep."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.micres.2015.06.012","article-title":"Klebsiella pneumoniae O antigen loss alters the outer membrane protein composition and the selective packaging of proteins into secreted outer membrane vesicles","volume":"180","author":"Cahill","year":"2015","journal-title":"Microbiol. Res. (Pavia)"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0030","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.12466","article-title":"Klebsiella pneumoniae survives within macrophages by avoiding delivery to lysosomes","author":"Cano","year":"2015","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0035","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1093\/jac\/dkq056","article-title":"Contribution of outer membrane protein K36 to antimicrobial resistance and virulence in Klebsiella pneumoniae","volume":"65","author":"Chen","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0040","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/15321810500403722","article-title":"A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells","volume":"27","author":"Choi","year":"2006","journal-title":"J. Immunoassay Immunochem."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0045","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.UTI-0005-2012","article-title":"Epidemiology and virulence of Klebsiella pneumoniae","volume":"4","author":"Clegg","year":"2016","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0050","doi-asserted-by":"crossref","first-page":"e10","DOI":"10.1093\/jpids\/pit049","article-title":"Microbiological and genetic characterization of carbapenem-resistant Klebsiella pneumoniae isolated from pediatric patients","volume":"3","author":"Dara","year":"2014","journal-title":"J. Pediatric Infect. Dis. Soc."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0055","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.bbapap.2008.11.005","article-title":"Outer membrane permeability and antibiotic resistance","volume":"1794","author":"Delcour","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0060","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/AAC.47.10.3332-3335.2003","article-title":"Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance","volume":"47","author":"Domenech-Sanchez","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0065","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1139\/m85-088","article-title":"Extracellular polysaccharide production by Klebsiella pneumoniae and its relationship to virulence","volume":"31","author":"Domenico","year":"1985","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0070","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.1128\/iai.57.12.3778-3782.1989","article-title":"Reduction of capsular polysaccharide production in Klebsiella pneumoniae by sodium salicylate","volume":"57","author":"Domenico","year":"1989","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0075","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1093\/jac\/dkp029","article-title":"Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK","volume":"63","author":"Doumith","year":"2009","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0080","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0969-2126(99)80055-0","article-title":"Crystal structure and functional characterization of OmpK36, the osmoporin of Klebsiella pneumoniae","volume":"7","author":"Dutzler","year":"1999","journal-title":"Structure"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0085","doi-asserted-by":"crossref","first-page":"4742","DOI":"10.1128\/AAC.00309-11","article-title":"OmpK26, a novel porin associated with carbapenem resistance in Klebsiella pneumoniae","volume":"55","author":"Garcia-Sureda","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0090","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1128\/AAC.01441-10","article-title":"Role of Klebsiella pneumoniae LamB Porin in antimicrobial resistance","volume":"55","author":"Garcia-Sureda","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0095","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1128\/JCM.37.1.56-62.1999","article-title":"Klebsiella pneumoniae lipopolysaccharide O typing: revision of prototype strains and O-group distribution among clinical isolates from different sources and countries","volume":"37","author":"Hansen","year":"1999","journal-title":"J. Clin. Microbiol."},{"issue":"Pt. 3","key":"10.1016\/j.ijmm.2019.04.001_bib0100","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1099\/13500872-145-3-673","article-title":"Porin expression in clinical isolates of Klebsiella pneumoniae","volume":"145","author":"Hernandez-Alles","year":"1999","journal-title":"Microbiology"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0105","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1128\/AAC.02411-12","article-title":"Characterization of porin expression in Klebsiella pneumoniae Carbapenemase (KPC)-producing K. pneumoniae identifies isolates most susceptible to the combination of colistin and carbapenems","volume":"57","author":"Hong","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0110","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0033155","article-title":"Lipopolysaccharide O1 antigen contributes to the virulence in Klebsiella pneumoniae causing pyogenic liver abscess","volume":"7","author":"Hsieh","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0115","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1093\/infdis\/jit384","article-title":"Klebsiella pneumoniae peptidoglycan-associated lipoprotein and murein lipoprotein contribute to serum resistance, antiphagocytosis, and proinflammatory cytokine stimulation","volume":"208","author":"Hsieh","year":"2013","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0120","series-title":"Capsule Stain Protocols","author":"Hughes","year":"2007"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0125","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1128\/iai.63.3.847-852.1995","article-title":"Relationships among capsular structure, phagocytosis, and mouse virulence in Klebsiella pneumoniae","volume":"63","author":"Kabha","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0130","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1046\/j.1462-2920.2001.00248.x","article-title":"Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7","volume":"3","author":"Kachlany","year":"2001","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0135","doi-asserted-by":"crossref","first-page":"3396","DOI":"10.1128\/AAC.00285-06","article-title":"High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of bla(ACT-1) beta-lactamase production, porin OmpK35\/36 insertional inactivation, and down-regulation of the phosphate transport porin phoe","volume":"50","author":"Kaczmarek","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0140","doi-asserted-by":"crossref","first-page":"895","DOI":"10.3389\/fmicb.2016.00895","article-title":"Global dissemination of carbapenemase-producing Klebsiella pneumoniae: epidemiology, genetic context, treatment options, and detection methods","volume":"7","author":"Lee","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0145","article-title":"Amino acid substitutions of MagA in Klebsiella pneumoniae affect the biosynthesis of the capsular polysaccharide","volume":"7","author":"Lin","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0150","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0155","doi-asserted-by":"crossref","first-page":"9956","DOI":"10.1074\/jbc.M110.181008","article-title":"Klebsiella pneumoniae outer membrane protein A is required to prevent the activation of airway epithelial cells","volume":"286","author":"March","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0160","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0056847","article-title":"Role of bacterial surface structures on the interaction of Klebsiella pneumoniae with phagocytes","volume":"8","author":"March","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0165","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1128\/iai.60.6.2529-2535.1992","article-title":"Mechanisms of Klebsiella pneumoniae resistance to complement-mediated killing","volume":"60","author":"Merino","year":"1992","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0170","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1128\/MMBR.67.4.593-656.2003","article-title":"Molecular basis of bacterial outer membrane permeability revisited","volume":"67","author":"Nikaido","year":"2003","journal-title":"Microbiol. Mol. Biol. Rev.: MMBR"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0175","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/MMBR.00078-15","article-title":"Klebsiella pneumoniae: going on the offense with a strong defense","volume":"80","author":"Paczosa","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev.: MMBR"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0180","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/AAC.01585-15","article-title":"Role of the outer membrane and porins in susceptibility of beta-lactamase-producing Enterobacteriaceae to ceftazidime-avibactam","volume":"60","author":"Pages","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0185","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1462-5822.2010.01526.x","article-title":"Klebsiella pneumoniae subverts the activation of inflammatory responses in a NOD1-dependent manner","volume":"13","author":"Regueiro","year":"2011","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0190","first-page":"1","article-title":"Prevalence of OmpK35 and OmpK36 porin expression in beta-lactamase and non-betalactamase- producing Klebsiella pneumoniae","volume":"6","author":"Shakib","year":"2012","journal-title":"Biol. Targets Ther."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0195","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1128\/IAI.72.3.1423-1430.2004","article-title":"The Klebsiella pneumoniae O antigen contributes to bacteremia and lethality during murine pneumonia","volume":"72","author":"Shankar-Sinha","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0200","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0041505","article-title":"Functional characterization of a novel outer membrane porin KpnO, regulated by PhoBR two-component system in Klebsiella pneumoniae NTUH-K2044","volume":"7","author":"Srinivasan","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ijmm.2019.04.001_bib0205","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1128\/AAC.01275-10","article-title":"Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence","volume":"55","author":"Tsai","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0210","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1128\/AAC.01627-15","article-title":"Porin loss impacts the host inflammatory response to outer membrane vesicles of Klebsiella pneumoniae","volume":"60","author":"Turner","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0215","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1128\/AAC.01627-15","article-title":"Porin loss impacts the host inflammatory response to outer membrane vesicles of Klebsiella pneumoniae","volume":"60","author":"Turner","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0220","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/j.1574-6968.2008.01208.x","article-title":"Evaluation of a multiplex PCR for detection of serotypes K1, K2 and K5 in Klebsiella sp. and comparison of isolates within these serotypes","volume":"284","author":"Turton","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0225","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1177\/1753425912459092","article-title":"Molecular basis for the increased polymyxin susceptibility of Klebsiella pneumoniae strains with under-acylated lipid A","volume":"19","author":"Velkov","year":"2013","journal-title":"Innate Immun."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0230","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0076-6879(86)18062-1","article-title":"Isolation and characterization of plant cell walls and cell-wall components","volume":"118","author":"York","year":"1986","journal-title":"Meth. Enzymol."},{"key":"10.1016\/j.ijmm.2019.04.001_bib0235","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1128\/AAC.02045-12","article-title":"Contribution of beta-lactamases and porin proteins OmpK35 and OmpK36 to carbapenem resistance in clinical isolates of KPC-2-producing Klebsiella pneumoniae","volume":"58","author":"Zhang","year":"2014","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["International Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422118303242?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422118303242?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T16:59:53Z","timestamp":1761411593000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1438422118303242"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":47,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["S1438422118303242"],"URL":"https:\/\/doi.org\/10.1016\/j.ijmm.2019.04.001","relation":{},"ISSN":["1438-4221"],"issn-type":[{"value":"1438-4221","type":"print"}],"subject":[],"published":{"date-parts":[[2019,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Porin loss in Klebsiella pneumoniae clinical isolates impacts production of virulence factors and survival within macrophages","name":"articletitle","label":"Article Title"},{"value":"International Journal of Medical Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijmm.2019.04.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}