{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:36:41Z","timestamp":1783039001438,"version":"3.54.6"},"reference-count":41,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Ministry of Science and ICT, Republic of Korea","award":["NRF-2016R1C1B2016275"],"award-info":[{"award-number":["NRF-2016R1C1B2016275"]}]},{"name":"Ministry of Science and ICT, Republic of Korea","award":["NRF-2019R1A2C4070284"],"award-info":[{"award-number":["NRF-2019R1A2C4070284"]}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Medical Microbiology"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.ijmm.2019.151338","type":"journal-article","created":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T00:01:26Z","timestamp":1565654486000},"page":"151338","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":53,"title":["Rapid diagnosis of bacterial meningitis by nanopore 16S amplicon sequencing: A pilot study"],"prefix":"10.1016","volume":"309","author":[{"given":"Jangsup","family":"Moon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae-Joon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Sun","family":"Jun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han Sang","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye-Rim","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon-Tae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keun-Hwa","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Il","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki-Young","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Kun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kon","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijmm.2019.151338_bib0005","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/nrmicro3380","article-title":"Molecular mechanisms of antibiotic resistance","volume":"13","author":"Blair","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151338_bib0010","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1016\/S0140-6736(12)61185-4","article-title":"Dilemmas in the diagnosis of acute community-acquired bacterial meningitis","volume":"380","author":"Brouwer","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.ijmm.2019.151338_bib0015","article-title":"Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens","volume":"9","author":"Chen","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ijmm.2019.151338_bib0020","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1542\/peds.2006-0440","article-title":"How reliable is a negative blood culture result? Volume of blood submitted for culture in routine practice in a children\u2019s hospital","volume":"119","author":"Connell","year":"2007","journal-title":"Pediatrics"},{"key":"10.1016\/j.ijmm.2019.151338_bib0025","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1056\/NEJM199301073280104","article-title":"Acute bacterial meningitis in adults\u2014a review of 493 episodes","volume":"328","author":"Durand","year":"1993","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.ijmm.2019.151338_bib0030","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13073-016-0356-2","article-title":"Mobile real-time surveillance of Zika virus in Brazil","volume":"8","author":"Faria","year":"2016","journal-title":"Genome Med."},{"key":"10.1016\/j.ijmm.2019.151338_bib0035","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/nrg.2017.88","article-title":"Towards a genomics-informed, real-time, global pathogen surveillance system","volume":"19","author":"Gardy","year":"2018","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.ijmm.2019.151338_bib0040","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1128\/CMR.5.2.130","article-title":"Laboratory diagnosis of bacterial meningitis","volume":"5","author":"Gray","year":"1992","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.ijmm.2019.151338_bib0045","doi-asserted-by":"crossref","DOI":"10.1186\/s13073-015-0220-9","article-title":"Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis","volume":"7","author":"Greninger","year":"2015","journal-title":"Genome Med."},{"key":"10.1016\/j.ijmm.2019.151338_bib0050","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1056\/NEJMoa1415627","article-title":"A variegated squirrel bornavirus associated with fatal human encephalitis","volume":"373","author":"Hoffmann","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.ijmm.2019.151338_bib0055","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1099\/jmm.0.030387-0","article-title":"Detection and identification of bacteria in clinical samples by 16S rRNA gene sequencing: comparison of two different approaches in clinical practice","volume":"61","author":"Jenkins","year":"2012","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151338_bib0060","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1002\/2211-5463.12590","article-title":"Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION\u2122 nanopore sequencer","volume":"9","author":"Kai","year":"2019","journal-title":"FEBS Open Bio"},{"key":"10.1016\/j.ijmm.2019.151338_bib0065","article-title":"Sources of PCR-induced distortions in high-throughput sequencing data sets","volume":"43","author":"Kebschull","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ijmm.2019.151338_bib0070","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s13742-015-0051-z","article-title":"Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer","volume":"4","author":"Kilianski","year":"2015","journal-title":"Gigascience"},{"key":"10.1016\/j.ijmm.2019.151338_bib0075","doi-asserted-by":"crossref","first-page":"760","DOI":"10.3201\/eid2105.150150","article-title":"Novel thogotovirus associated with febrile illness and death, United States, 2014","volume":"21","author":"Kosoy","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151338_bib0080","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/CMR.00110-14","article-title":"Current and past strategies for bacterial culture in clinical microbiology","volume":"28","author":"Lagier","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.ijmm.2019.151338_bib0085","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1128\/JCM.44.4.1250-1256.2006","article-title":"Characteristic signatures of the lytA gene provide a basis for rapid and reliable diagnosis of Streptococcus pneumoniae infections","volume":"44","author":"Llull","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151338_bib0090","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1038\/nmeth.3625","article-title":"Nanopores: a sequencer in your backpack","volume":"12","author":"Marx","year":"2015","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ijmm.2019.151338_bib0095","doi-asserted-by":"crossref","first-page":"3036","DOI":"10.1016\/S0140-6736(16)30654-7","article-title":"Acute bacterial meningitis in adults","volume":"388","author":"McGill","year":"2016","journal-title":"Lancet"},{"key":"10.1016\/j.ijmm.2019.151338_bib0100","doi-asserted-by":"crossref","first-page":"5657","DOI":"10.1038\/s41598-017-05772-5","article-title":"A portable system for rapid bacterial composition analysis using a nanopore-based sequencer and laptop computer","volume":"7","author":"Mitsuhashi","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijmm.2019.151338_bib0105","doi-asserted-by":"crossref","first-page":"1944","DOI":"10.3201\/eid2410.180234","article-title":"Diagnosis of Haemophilus influenzae pneumonia by nanopore 16S amplicon sequencing of sputum","volume":"24","author":"Moon","year":"2018","journal-title":"Emerging Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151338_bib0110","doi-asserted-by":"crossref","first-page":"e94","DOI":"10.1038\/emi.2017.81","article-title":"Campylobacter fetus meningitis confirmed by a 16S rRNA gene analysis using the MinION nanopore sequencer, South Korea, 2016","volume":"6","author":"Moon","year":"2017","journal-title":"Emerg. Microbes Infect."},{"key":"10.1016\/j.ijmm.2019.151338_bib0115","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1093\/cid\/ciu912","article-title":"Diagnosis of neuroinvasive astrovirus infection in an immunocompromised adult with encephalitis by unbiased next-generation sequencing","volume":"60","author":"Naccache","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151338_bib0120","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1164\/rccm.201703-0537LE","article-title":"Rapid pathogen identification in bacterial pneumonia using real-time metagenomics","volume":"196","author":"Pendleton","year":"2017","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.ijmm.2019.151338_bib0125","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1093\/qjmed\/hci047","article-title":"Delays in the administration of antibiotics are associated with mortality from adult acute bacterial meningitis","volume":"98","author":"Proulx","year":"2005","journal-title":"QJM"},{"key":"10.1016\/j.ijmm.2019.151338_bib0130","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1038\/nprot.2017.066","article-title":"Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples","volume":"12","author":"Quick","year":"2017","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ijmm.2019.151338_bib0135","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nature16996","article-title":"Real-time, portable genome sequencing for Ebola surveillance","volume":"530","author":"Quick","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.ijmm.2019.151338_bib0140","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-26334-3","article-title":"Nanopore sequencing of drug-resistance-associated genes in malaria parasites, Plasmodium falciparum","volume":"8","author":"Runtuwene","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijmm.2019.151338_bib0145","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0065226","article-title":"Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections","volume":"8","author":"Salipante","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ijmm.2019.151338_bib0150","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1186\/s12864-018-5094-y","article-title":"Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices","volume":"19","author":"Sanderson","year":"2018","journal-title":"BMC Genomics"},{"key":"10.1016\/j.ijmm.2019.151338_bib0155","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1128\/JCM.42.2.734-740.2004","article-title":"Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting","volume":"42","author":"Schuurman","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151338_bib0160","doi-asserted-by":"crossref","first-page":"29681","DOI":"10.1038\/srep29681","article-title":"Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing","volume":"6","author":"Shin","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijmm.2019.151338_bib0165","doi-asserted-by":"crossref","first-page":"6589","DOI":"10.1038\/s41598-017-06665-3","article-title":"Large-scale differences in microbial biodiversity discovery between 16S amplicon and shotgun sequencing","volume":"7","author":"Tessler","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijmm.2019.151338_bib0170","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1086\/425368","article-title":"Practice guidelines for the management of bacterial meningitis","volume":"39","author":"Tunkel","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151338_bib0175","doi-asserted-by":"crossref","first-page":"10931","DOI":"10.1038\/s41598-018-29334-5","article-title":"Evaluation of oxford nanopore\u2019s MinION sequencing device for microbial whole genome sequencing applications","volume":"8","author":"Tyler","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijmm.2019.151338_bib0180","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1016\/S0140-6736(12)61808-X","article-title":"Progress and challenges in bacterial meningitis","volume":"380","author":"van de Beek","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.ijmm.2019.151338_bib0185","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1016\/S0140-6736(12)61186-6","article-title":"Advances in treatment of bacterial meningitis","volume":"380","author":"van de Beek","year":"2012","journal-title":"Lancet"},{"key":"10.1016\/j.ijmm.2019.151338_bib0190","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1111\/j.1469-0691.2007.01756.x","article-title":"Comparison of broad-range bacterial PCR and culture of cerebrospinal fluid for diagnosis of community-acquired bacterial meningitis","volume":"13","author":"Welinder-Olsson","year":"2007","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ijmm.2019.151338_bib0195","doi-asserted-by":"crossref","first-page":"2408","DOI":"10.1056\/NEJMoa1401268","article-title":"Actionable diagnosis of neuroleptospirosis by next-generation sequencing","volume":"370","author":"Wilson","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.ijmm.2019.151338_bib0200","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1001\/jamaneurol.2018.0463","article-title":"Chronic meningitis investigated via metagenomic next-generation sequencing","volume":"75","author":"Wilson","year":"2018","journal-title":"JAMA Neurol."},{"key":"10.1016\/j.ijmm.2019.151338_bib0205","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1111\/j.1469-0691.2008.02070.x","article-title":"Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories","volume":"14","author":"Woo","year":"2008","journal-title":"Clin. Microbiol. Infect."}],"container-title":["International Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422119301456?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422119301456?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T07:14:58Z","timestamp":1566976498000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1438422119301456"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["S1438422119301456"],"URL":"https:\/\/doi.org\/10.1016\/j.ijmm.2019.151338","relation":{},"ISSN":["1438-4221"],"issn-type":[{"value":"1438-4221","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid diagnosis of bacterial meningitis by nanopore 16S amplicon sequencing: A pilot study","name":"articletitle","label":"Article Title"},{"value":"International Journal of Medical Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijmm.2019.151338","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"151338"}}