{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:49:22Z","timestamp":1790066962858,"version":"4.0.1"},"reference-count":24,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T00:00:00Z","timestamp":1573171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021570","name":"Ministerie van Defensie","doi-asserted-by":"publisher","award":["V1408"],"award-info":[{"award-number":["V1408"]}],"id":[{"id":"10.13039\/501100021570","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001199","name":"Fellowships Fund Incorporated","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003400","name":"Ontario Ministry of Research, Innovation and Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003400","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006021","name":"Direction G\u00e9n\u00e9rale de l\u2019Armement","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006021","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006083","name":"Ministry of Defence","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006083","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008757","name":"Totalf\u00f6rsvarets Forskningsinstitut","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008757","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Medical Microbiology"],"published-print":{"date-parts":[[2020,1]]},"DOI":"10.1016\/j.ijmm.2019.151376","type":"journal-article","created":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T11:39:23Z","timestamp":1573472363000},"page":"151376","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["Untargeted accurate identification of highly pathogenic bacteria directly from blood culture flasks"],"prefix":"10.1016","volume":"310","author":[{"given":"Erwin M.","family":"Berendsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeni","family":"Levin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Braakman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Prodan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans C.","family":"van Leeuwen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armand","family":"Paauw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijmm.2019.151376_bib0005","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.2217\/fmb-2017-0050","article-title":"Identification of microorganisms grown in blood culture flasks using liquid chromatography-tandem mass spectrometry","volume":"12","author":"Berendsen","year":"2017","journal-title":"Future Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0010","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1074\/mcp.M116.061721","article-title":"Typing and characterization of bacteria using bottom-up tandem mass spectrometry proteomics","volume":"16","author":"Boulund","year":"2017","journal-title":"Mol. Cell Proteomics"},{"key":"10.1016\/j.ijmm.2019.151376_bib0015","first-page":"762","article-title":"Fast string searching algorithm","volume":"20","author":"Boyer","year":"1977"},{"key":"10.1016\/j.ijmm.2019.151376_bib0020","series-title":"Shiny: Web Application Framework for R. R Package Version 1.0.5","author":"Chang","year":"2017"},{"key":"10.1016\/j.ijmm.2019.151376_bib0025","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1086\/313586","article-title":"Detection of selected fastidious bacteria","volume":"30","author":"Doern","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151376_bib0030","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0732-8893(96)00018-1","article-title":"Lack of requirement for prolonged incubation of Septi-Chek blood culture bottles in patients with bacteremia due to fastidious bacteria","volume":"24","author":"Doern","year":"1996","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151376_bib0035","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1007\/s10096-017-2975-y","article-title":"Direct detection of extended-spectrum beta-lactamases (CTX-M) from blood cultures by LC-MS\/MS bottom-up proteomics","volume":"36","author":"Fleurbaaij","year":"2017","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151376_bib0040","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.pathol.2019.01.006","article-title":"Yersinia pseudotuberculosis bacteraemia: a diagnostic dilemma in the era of MALDI-TOF mass spectrometry","volume":"51","author":"Harch","year":"2019","journal-title":"Pathology"},{"key":"10.1016\/j.ijmm.2019.151376_bib0045","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01437-18","article-title":"Utility of a rapid lateral flow assay to resolve erroneous identification of Burkholderia pseudomallei as Burkholderia thailandensis by Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry","volume":"56","author":"Harch","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0050","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1021\/pr101086a","article-title":"A protein processing filter method for bacterial identification by mass spectrometry-based proteomics","volume":"10","author":"Jabbour","year":"2011","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.ijmm.2019.151376_bib0055","doi-asserted-by":"crossref","first-page":"3637","DOI":"10.1128\/AEM.00055-10","article-title":"Double-blind characterization of non-genome-sequenced bacteria by mass spectrometry-based proteomics","volume":"76","author":"Jabbour","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0060","doi-asserted-by":"crossref","first-page":"e8041","DOI":"10.1371\/journal.pone.0008041","article-title":"Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry","volume":"4","author":"La Scola","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.ijmm.2019.151376_bib0065","doi-asserted-by":"crossref","first-page":"697","DOI":"10.3389\/fmicb.2016.00697","article-title":"How to optimize the use of blood cultures for the diagnosis of bloodstream infections? A state-of-the art","volume":"7","author":"Lamy","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0070","doi-asserted-by":"crossref","first-page":"2632","DOI":"10.1128\/JCM.00813-15","article-title":"Identification of highly pathogenic microorganisms by matrix-assisted laser desorption ionization-time of flight mass spectrometry: results of an interlaboratory ring trial","volume":"53","author":"Lasch","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0075","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.jmii.2017.03.005","article-title":"A simple method for rapid microbial identification from positive monomicrobial blood culture bottles through matrix-assisted laser desorption ionization time-of-flight mass spectrometry","volume":"51","author":"Lin","year":"2018","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.ijmm.2019.151376_bib0080","doi-asserted-by":"crossref","first-page":"3513","DOI":"10.1128\/JCM.01023-17","article-title":"Safety and accuracy of matrix-assisted laser desorption ionization-time of flight mass Spectrometry for identification of highly pathogenic organisms","volume":"55","author":"Rudrik","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0085","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01278-18","article-title":"Direct identification of 80 percent of bacteria from blood culture bottles by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry using a 10-Minute extraction protocol","volume":"57","author":"Simon","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0090","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1099\/ijsem.0.001572","volume":"67","author":"Tindall","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0095","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.mimet.2013.04.008","article-title":"A simple shotgun proteomics method for rapid bacterial identification","volume":"94","author":"Tracz","year":"2013","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ijmm.2019.151376_bib0100","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1128\/JCM.00620-15","article-title":"Simultaneous identification of multiple beta-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry","volume":"53","author":"Trip","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0105","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis","volume":"6","author":"Wisniewski","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ijmm.2019.151376_bib0110","doi-asserted-by":"crossref","first-page":"3437","DOI":"10.1128\/JCM.37.11.3437-3442.1999","article-title":"Detection of Brucellae in blood cultures","volume":"37","author":"Yagupsky","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ijmm.2019.151376_bib0115","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ijid.2016.09.014","article-title":"Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method","volume":"52","author":"Yonetani","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"10.1016\/j.ijmm.2019.151376_bib0120","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M111.010587","article-title":"PEAKS DB: de novo sequencing assisted database search for sensitive and accurate peptide identification","volume":"11","author":"Zhang","year":"2012","journal-title":"Mol. Cell Proteomics"}],"container-title":["International Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422119303170?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1438422119303170?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T17:00:31Z","timestamp":1761411631000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1438422119303170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["S1438422119303170"],"URL":"https:\/\/doi.org\/10.1016\/j.ijmm.2019.151376","relation":{},"ISSN":["1438-4221"],"issn-type":[{"value":"1438-4221","type":"print"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Untargeted accurate identification of highly pathogenic bacteria directly from blood culture flasks","name":"articletitle","label":"Article Title"},{"value":"International Journal of Medical Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijmm.2019.151376","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 The Authors. Published by Elsevier GmbH.","name":"copyright","label":"Copyright"}],"article-number":"151376"}}