{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:42:59Z","timestamp":1773211379717,"version":"3.50.1"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"publisher","award":["202201AY070001-200"],"award-info":[{"award-number":["202201AY070001-200"]}],"id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Indian Journal of Medical Microbiology"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.ijmmb.2025.100977","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T21:27:13Z","timestamp":1758576433000},"page":"100977","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Establishment of a multiplex real-time quantitative PCR assay using TaqMan fluorescent probes to detect Mucorales species"],"prefix":"10.1016","volume":"58","author":[{"given":"Yongsheng","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Pu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongren","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerong","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minfeng","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoliang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunping","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limin","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijmmb.2025.100977_bib1","article-title":"Review on mucormycosis: pathogenesis, epidemiology, microbiology and diagnosis","volume":"24","author":"Sharma","year":"2024","journal-title":"Infect Disord: Drug Targets"},{"key":"10.1016\/j.ijmmb.2025.100977_bib2","doi-asserted-by":"crossref","DOI":"10.1080\/07853890.2024.2396570","article-title":"Epidemiology, pathogenesis, clinical characteristics, and treatment of mucormycosis: a review","volume":"56","author":"Liang","year":"2024","journal-title":"Ann Med"},{"key":"10.1016\/j.ijmmb.2025.100977_bib3","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232315066","article-title":"A rapid and specific real-time PCR assay for the detection of clinically relevant Mucorales species","volume":"23","author":"Bergallo","year":"2022","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.ijmmb.2025.100977_bib4","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01230-21","article-title":"Epidemiology and antifungal susceptibilities of mucoralean fungi in clinical samples from the United States","volume":"59","author":"Badali","year":"2021","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijmmb.2025.100977_bib5","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cmi.2018.07.011","article-title":"The epidemiology and clinical manifestations of mucormycosis: a systematic review and meta-analysis of case reports","volume":"25","author":"Jeong","year":"2019","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.ijmmb.2025.100977_bib6","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1093\/mmy\/myy060","article-title":"A prospective multicenter study on mucormycosis in India: epidemiology, diagnosis, and treatment","volume":"57","author":"Prakash","year":"2019","journal-title":"Med Mycol"},{"key":"10.1016\/j.ijmmb.2025.100977_bib7","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1273073","article-title":"Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR","volume":"14","author":"Xu","year":"2023","journal-title":"Front Microbiol"},{"key":"10.1016\/j.ijmmb.2025.100977_bib8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.3390\/jof6040265","article-title":"Epidemiology and diagnosis of mucormycosis: an update","volume":"6","author":"Skiada","year":"2020","journal-title":"J Fungi (Basel)"},{"key":"10.1016\/j.ijmmb.2025.100977_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11046-021-00607-4","article-title":"Mucormycosis in mainland China: a systematic review of case reports","volume":"187","author":"Wei","year":"2022","journal-title":"Mycopathologia"},{"key":"10.1016\/j.ijmmb.2025.100977_bib10","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1111\/j.1469-0691.2010.03456.x","article-title":"Zygomycosis in Europe: analysis of 230 cases accrued by the registry of the European confederation of medical Mycology (ECMM) working group on Zygomycosis between 2005 and 2007","volume":"17","author":"Skiada","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.ijmmb.2025.100977_bib11","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1186\/1471-2334-14-310","article-title":"Hospital days, hospitalization costs, and inpatient mortality among patients with mucormycosis: a retrospective analysis of US hospital discharge data","volume":"14","author":"Zilberberg","year":"2014","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.ijmmb.2025.100977_bib12","article-title":"Successful treatment of rhino-cerebral mucormycosis with dual antifungal therapy and minimal surgical debridement","volume":"30","author":"Bougioukas","year":"2022","journal-title":"ID Cases"},{"key":"10.1016\/j.ijmmb.2025.100977_bib13","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1093\/mmy\/myx101","article-title":"Challenges in the diagnosis and treatment of mucormycosis","volume":"56","author":"Skiada","year":"2018","journal-title":"Med Mycol"},{"key":"10.1016\/j.ijmmb.2025.100977_bib14","doi-asserted-by":"crossref","first-page":"264.e5","DOI":"10.1016\/j.ajem.2020.09.032","article-title":"Mucormycosis with orbital compartment syndrome in a patient with COVID-19","volume":"42","author":"Werthman-Ehrenreich","year":"2021","journal-title":"Am J Emerg Med"},{"key":"10.1016\/j.ijmmb.2025.100977_bib15","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00746-18","article-title":"PCR-based approach targeting mucorales-specific gene family for diagnosis of mucormycosis","volume":"56","author":"Baldin","year":"2018","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijmmb.2025.100977_bib16","doi-asserted-by":"crossref","first-page":"e95","DOI":"10.1093\/cid\/cit094","article-title":"Quantitative polymerase chain reaction detection of circulating DNA in serum for early diagnosis of mucormycosis in immunocompromised patients","volume":"56","author":"Millon","year":"2013","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.ijmmb.2025.100977_bib17","doi-asserted-by":"crossref","first-page":"457","DOI":"10.3390\/jof8050457","article-title":"Recent developments in the diagnosis of mucormycosis","volume":"8","author":"Dannaoui","year":"2022","journal-title":"J Fungi (Basel)"},{"key":"10.1016\/j.ijmmb.2025.100977_bib18","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3390\/jof5010024","article-title":"Molecular strategies to diagnose mucormycosis","volume":"5","author":"Millon","year":"2019","journal-title":"J Fungi (Basel)"},{"key":"10.1016\/j.ijmmb.2025.100977_bib19","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/cid\/ciab1066","article-title":"Evaluation of serum Mucorales polymerase chain reaction (PCR) for the diagnosis of mucormycoses: the MODIMUCOR prospective trial","volume":"75","author":"Millon","year":"2022","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.ijmmb.2025.100977_bib20","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.1128\/JCM.00917-08","article-title":"Detection of a molecular biomarker for Zygomycetes by quantitative PCR assays of plasma, bronchoalveolar lavage, and lung tissue in a rabbit model of experimental pulmonary zygomycosis","volume":"46","author":"Kasai","year":"2008","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.ijmmb.2025.100977_bib21","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/978-1-4939-6730-8_8","article-title":"Multiplex analyses using real-time quantitative PCR","volume":"1546","author":"Hawkins","year":"2017","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.ijmmb.2025.100977_bib22","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.00634-24","article-title":"Development of a TaqMan probe-based multiplex real-time PCR for the simultaneous detection of four clinically important filamentous fungi","volume":"12","author":"Wei","year":"2024","journal-title":"Microbiol Spectr"},{"key":"10.1016\/j.ijmmb.2025.100977_bib23","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1086\/432579","article-title":"Epidemiology and outcome of zygomycosis: a review of 929 reported cases","volume":"41","author":"Roden","year":"2005","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.ijmmb.2025.100977_bib24","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/jof5010023","article-title":"Disease entities in mucormycosis","volume":"5","author":"Serris","year":"2019","journal-title":"J Fungi (Basel)"}],"container-title":["Indian Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0255085725001902?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0255085725001902?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T08:58:41Z","timestamp":1773133121000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0255085725001902"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":24,"alternative-id":["S0255085725001902"],"URL":"https:\/\/doi.org\/10.1016\/j.ijmmb.2025.100977","relation":{},"ISSN":["0255-0857"],"issn-type":[{"value":"0255-0857","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Establishment of a multiplex real-time quantitative PCR assay using TaqMan fluorescent probes to detect Mucorales species","name":"articletitle","label":"Article Title"},{"value":"Indian Journal of Medical Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijmmb.2025.100977","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V. on behalf of Indian Association of Medical Microbiologists.","name":"copyright","label":"Copyright"}],"article-number":"100977"}}