{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T04:56:24Z","timestamp":1773982584684,"version":"3.50.1"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100032391","name":"Veer Narmad South Gujarat University","doi-asserted-by":"publisher","award":["UGC\/17097\/2023"],"award-info":[{"award-number":["UGC\/17097\/2023"]}],"id":[{"id":"10.13039\/100032391","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100032391","name":"Veer Narmad South Gujarat University","doi-asserted-by":"publisher","award":["05-07-2023"],"award-info":[{"award-number":["05-07-2023"]}],"id":[{"id":"10.13039\/100032391","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Indian Journal of Medical Microbiology"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.ijmmb.2026.101047","type":"journal-article","created":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T07:38:56Z","timestamp":1768030736000},"page":"101047","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Development of an enhanced LAMP-based method for early identification of Streptococcus pneumoniae through LytA gene amplification"],"prefix":"10.1016","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0330-0132","authenticated-orcid":false,"given":"Jignisha S.","family":"Patel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3200-3356","authenticated-orcid":false,"given":"Jigna P.","family":"Naik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2985-6710","authenticated-orcid":false,"given":"Dhruv N.","family":"Desai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.ijmmb.2026.101047_bib1","doi-asserted-by":"crossref","DOI":"10.1080\/21645515.2025.2461844","article-title":"Streptococcus pneumoniae serotype distribution in low- and middle-income countries of South Asia: do we need to revisit the pneumococcal vaccine strategy?","volume":"21","author":"Dhawale","year":"2025","journal-title":"Hum Vaccin Immunother"},{"key":"10.1016\/j.ijmmb.2026.101047_bib2","doi-asserted-by":"crossref","DOI":"10.3389\/fpubh.2025.1501821","article-title":"Streptococcus pneumoniae serotypes and factors associated with antimicrobial resistance in invasive pneumococcal disease cases in Latvia, 2012\u20132022","volume":"13","author":"Savrasova","year":"2025","journal-title":"Front Public Health"},{"issue":"1","key":"10.1016\/j.ijmmb.2026.101047_bib3","doi-asserted-by":"crossref","DOI":"10.3201\/eid3001.221927","article-title":"Disease-associated Streptococcus pneumoniae genetic variation","volume":"30","author":"Yang","year":"2024","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.ijmmb.2026.101047_bib4","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.866259","article-title":"Streptococcus pneumoniae strains isolated from a single pediatric patient display distinct phenotypes","volume":"12","author":"Agnew","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.ijmmb.2026.101047_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.949469","article-title":"Non-capsular based immunization approaches to prevent Streptococcus pneumoniae infection","volume":"12","author":"Silva","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.ijmmb.2026.101047_bib6","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.mimet.2017.07.015","article-title":"Current challenges in the accurate identification of Streptococcus pneumoniae and its serogroups\/serotypes in the vaccine era","volume":"141","author":"Varghese","year":"2017","journal-title":"J Microbiol Methods"},{"issue":"6","key":"10.1016\/j.ijmmb.2026.101047_bib7","doi-asserted-by":"crossref","first-page":"e735","DOI":"10.1016\/S2214-109X(19)30081-6","article-title":"National, regional, and state-level burden of Streptococcus pneumoniae and Haemophilus influenzae type b disease in children in India: modelled estimates for 2000\u201315","volume":"7","author":"Wahl","year":"2019","journal-title":"Lancet Glob Health"},{"issue":"5","key":"10.1016\/j.ijmmb.2026.101047_bib8","article-title":"Review on pneumococcal infection in children","volume":"13","author":"Thadchanamoorthy","year":"2021","journal-title":"Cureus"},{"issue":"5","key":"10.1016\/j.ijmmb.2026.101047_bib9","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.jmii.2018.03.004","article-title":"Invasive pneumococcal disease in Indian adults: 11 years' experience","volume":"52","author":"Jayaraman","year":"2018","journal-title":"J Microbiol Immunol Infect"},{"issue":"4","key":"10.1016\/j.ijmmb.2026.101047_bib10","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0732-8893(01)00358-3","article-title":"Possible overestimation of penicillin resistant Streptococcus pneumoniae colonization rates due to misidentification of oropharyngeal streptococci","volume":"42","author":"Wester","year":"2002","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"8","key":"10.1016\/j.ijmmb.2026.101047_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0042954","article-title":"The enhanced pneumococcal LAMP assay: a clinical tool for the diagnosis of Meningitis due to Streptococcus pneumoniae","volume":"7","author":"Kim","year":"2012","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.ijmmb.2026.101047_bib12","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-39791-1","article-title":"Identification of Streptococcus pneumoniae by a real-time PCR assay targeting SP2020","volume":"9","author":"Tavares","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.ijmmb.2026.101047_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.ebiom.2021.103274","article-title":"Molecular diagnostic assays for the detection of common bacterial meningitis pathogens: a narrative review","volume":"65","author":"Diallo","year":"2021","journal-title":"EBioMedicine"},{"issue":"12","key":"10.1016\/j.ijmmb.2026.101047_bib14","doi-asserted-by":"crossref","first-page":"63e","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids"},{"issue":"3","key":"10.1016\/j.ijmmb.2026.101047_bib15","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop primers","volume":"16","author":"Nagamine","year":"2002","journal-title":"Mol Cell Probes"},{"key":"10.1016\/j.ijmmb.2026.101047_bib16","article-title":"Recent advances in loop-mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens","volume":"3","author":"Garg","year":"2022","journal-title":"Curr Res Microb Sci"},{"issue":"6","key":"10.1016\/j.ijmmb.2026.101047_bib17","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/rmv.593","article-title":"Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases","volume":"18","author":"Parida","year":"2008","journal-title":"Rev Med Virol"},{"issue":"9","key":"10.1016\/j.ijmmb.2026.101047_bib18","first-page":"DC06","article-title":"Development of gene-based conventional PCR assay for diagnostic application of Streptococcus pneumoniae infections as an alternative to conventional culture: a cross-sectional StudyJ","volume":"19","author":"Patel","year":"2025","journal-title":"Clin Diagn Res"},{"key":"10.1016\/j.ijmmb.2026.101047_bib19","series-title":"Medical microbiology E-Book: medical microbiology E-Book","author":"Murray","year":"2020"},{"key":"10.1016\/j.ijmmb.2026.101047_bib20","series-title":"Koneman's color Atlas and textbook of diagnostic microbiology","author":"Koneman","year":"2006"},{"key":"10.1016\/j.ijmmb.2026.101047_bib21","series-title":"Shigella, and Vibrio cholerae","year":"2003"},{"key":"10.1016\/j.ijmmb.2026.101047_bib23","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.17776","article-title":"Development and evaluation of a rapid visual loop-mediated isothermal amplification assay for the tcdA gene in Clostridioides difficile detection","volume":"12","author":"Lin","year":"2024","journal-title":"PeerJ"},{"issue":"4","key":"10.1016\/j.ijmmb.2026.101047_bib24","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1128\/JCM.43.4.1581-1586.2005","article-title":"Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae","volume":"43","author":"Seki","year":"2005","journal-title":"J Clin Microbiol"}],"container-title":["Indian Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0255085726000058?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0255085726000058?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T03:48:52Z","timestamp":1773978532000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0255085726000058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":23,"alternative-id":["S0255085726000058"],"URL":"https:\/\/doi.org\/10.1016\/j.ijmmb.2026.101047","relation":{},"ISSN":["0255-0857"],"issn-type":[{"value":"0255-0857","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of an enhanced LAMP-based method for early identification of Streptococcus pneumoniae through LytA gene amplification","name":"articletitle","label":"Article Title"},{"value":"Indian Journal of Medical Microbiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijmmb.2026.101047","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"101047"}}