{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:18:36Z","timestamp":1788542316405,"version":"build-2803163510"},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,3,6]],"date-time":"2016-03-06T00:00:00Z","timestamp":1457222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005068","name":"Jimma University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec>\n                    <jats:title>Objective\/background:<\/jats:title>\n                    <jats:p>\n                      The nature and frequency of mutations in rifampicin (RIF) and isoniazid (INH) resistant\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      isolates vary considerably according to geographic locations. However, information regarding specific mutational patterns in Ethiopia remains limited.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      A cross-sectional prospective study was carried out among confirmed pulmonary tuberculosis cases in Southwest Ethiopia. Mutations associated with RIF and INH resistances were studied using GenoType MTBDRplus line probe assay in 112\n                      <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                      isolates. Culture (MGIT960) and identification tests were performed at the Mycobacteriology Research Center of Jimma University, Jimma, Ethiopia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Mutations conferring resistance to INH, RIF, and multidrug resistance were detected in 36.6% (41\/112), 30.4% (34\/112), and 27.7% (31\/112) of\n                      <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                      isolates respectively. Among 34 RIF-resistant isolates, 82.4% (28\/34) had\n                      <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                      gene mutations at S531L, 2.9% (1\/34) at H526D, and 14.7% (5\/34) had mutations only at wild type probes. Of 41 INH-resistant strains, 87.8% (36\/41) had mutations in the katG gene at Ser315Thr1 and 9.8% (4\/41) had mutations in the inhA gene at C15T. Mutations in inhA promoter region were strongly associated with INH monoresistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      A high rate of drug resistance was commonly observed among failure cases. The most frequent gene mutations associated with the resistance to INH and RIF were observed in the codon 315 of the\n                      <jats:italic toggle=\"yes\">katG<\/jats:italic>\n                      gene and codon 531 of the\n                      <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                      gene, respectively. Further studies on mutations in different geographic regions using DNA sequencing techniques are warranted to improve the kit by including more specific mutation probes in the kit.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1016\/j.ijmyco.2016.02.009","type":"journal-article","created":{"date-parts":[[2016,3,11]],"date-time":"2016-03-11T09:10:13Z","timestamp":1457687413000},"page":"185-191","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":39,"title":["Drug resistance-conferring mutations in Mycobacterium tuberculosis from pulmonary tuberculosis patients in Southwest Ethiopia"],"prefix":"10.4103","volume":"5","author":[{"given":"Mulualem","family":"Tadesse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dossegnaw","family":"Aragaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belayneh","family":"Dimah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feyisa","family":"Efa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kedir","family":"Abdella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wakjira","family":"Kebede","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ketema","family":"Abdissa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemeda","family":"Abebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R5-11-20260904","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1128\/JCM.00521-07","article-title":"Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens","volume":"45","author":"Hillemann","year":"2007","journal-title":"J. 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