{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:25:48Z","timestamp":1793316348038,"version":"4.5.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T00:00:00Z","timestamp":1424995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBSRC\/BB\/I011218\/1"],"award-info":[{"award-number":["BBSRC\/BB\/I011218\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P40 OD010440"],"award-info":[{"award-number":["P40 OD010440"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology"],"published-print":{"date-parts":[[2015,4]]},"DOI":"10.1016\/j.ijpara.2015.01.004","type":"journal-article","created":{"date-parts":[[2015,2,28]],"date-time":"2015-02-28T16:01:17Z","timestamp":1425139277000},"page":"345-355","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["A highly conserved, inhibitable astacin metalloprotease from Teladorsagia circumcincta is required for cuticle formation and nematode development"],"prefix":"10.1016","volume":"45","author":[{"given":"Gillian","family":"Stepek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"McCormack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan D.","family":"Winter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antony P.","family":"Page","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijpara.2015.01.004_b0005","doi-asserted-by":"crossref","first-page":"6562","DOI":"10.1016\/j.bmcl.2008.10.036","article-title":"Succinyl hydroxamates as potent and selective non-peptidic inhibitors of procollagen C-proteinase: design, synthesis, and evaluation as topically applied, dermal anti-scarring agents","volume":"18","author":"Bailey","year":"2008","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.ijpara.2015.01.004_b0010","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.vetpar.2011.11.026","article-title":"Expression profiling, gene silencing and transcriptional networking of metzincin metalloproteases in the cattle tick, Rhipicephalus (Boophilus) microplus","volume":"186","author":"Barnard","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0015","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.pt.2006.11.004","article-title":"New anthelmintics for livestock: the time is right","volume":"23","author":"Besier","year":"2007","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0020","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1038\/358164a0","article-title":"Structure of astacin and implications for activation of astacins and zinc-ligation of collagenases","volume":"358","author":"Bode","year":"1992","journal-title":"Nature"},{"key":"10.1016\/j.ijpara.2015.01.004_b0025","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1002\/pro.5560040701","article-title":"The astacin family of metalloendopeptidases","volume":"4","author":"Bond","year":"1995","journal-title":"Protein Sci."},{"key":"10.1016\/j.ijpara.2015.01.004_b0030","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0304-4017(01)00430-7","article-title":"Parasite vaccines \u2013 a reality?","volume":"98","author":"Dalton","year":"2001","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0035","doi-asserted-by":"crossref","first-page":"6001","DOI":"10.1242\/dev.01454","article-title":"A conserved metalloprotease mediates ecdysis in Caenorhabditis elegans","volume":"131","author":"Davis","year":"2004","journal-title":"Development"},{"key":"10.1016\/j.ijpara.2015.01.004_b0040","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1086\/524070","article-title":"Hookworm vaccines","volume":"46","author":"Diemert","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ijpara.2015.01.004_b0045","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.pt.2013.01.001","article-title":"New advancement in anthelmintic drugs in veterinary medicine","volume":"29","author":"Epe","year":"2013","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0050","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/0305-0491(89)90360-X","article-title":"The cuticular proteins from free-living and parasitic stages of Haemonchus contortus\u2013I. Isolation and partial characterization","volume":"94","author":"Fetterer","year":"1989","journal-title":"Comp. Biochem. Physiol. B"},{"key":"10.1016\/j.ijpara.2015.01.004_b0055","doi-asserted-by":"crossref","first-page":"3442","DOI":"10.1021\/jm061010z","article-title":"Potent and selective nonpeptidic inhibitors of procollagen C-proteinase","volume":"50","author":"Fish","year":"2007","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpara.2015.01.004_b0060","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.parint.2005.02.002","article-title":"Identification of an astacin-like metallo-proteinase transcript from the infective larvae of Strongyloides stercoralis","volume":"54","author":"Gallego","year":"2005","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ijpara.2015.01.004_b0065","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0166-6851(89)90041-8","article-title":"Purification of a 44kilodalton protease which mediates the ecdysis of infective Haemonchus contortus larvae","volume":"33","author":"Gamble","year":"1989","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0070","doi-asserted-by":"crossref","first-page":"197","DOI":"10.2307\/3284145","article-title":"Developmentally regulated zinc metalloproteinases from third- and fourth-stage larvae of the ovine nematode Haemonchus contortus","volume":"82","author":"Gamble","year":"1996","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0075","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/978-1-60327-411-1_26","article-title":"Allele-specific PCR in SNP genotyping","volume":"578","author":"Gaudet","year":"2009","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0080","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1039\/p19750000819","article-title":"Studies concerning the antibiotic actinonin. Part I. The constitution of actinonin. A natural hydroxamic acid with antibiotic activity","volume":"1","author":"Gordon","year":"1975","journal-title":"J. Chem. Soc. Perkin"},{"key":"10.1016\/j.ijpara.2015.01.004_b0085","doi-asserted-by":"crossref","first-page":"13958","DOI":"10.1074\/jbc.M109.097436","article-title":"Proenzyme structure and activation of astacin metallopeptidase","volume":"285","author":"Guevara","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijpara.2015.01.004_b0090","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1017\/S0031182011002010","article-title":"Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status","volume":"139","author":"Halliday","year":"2012","journal-title":"Parasitology"},{"key":"10.1016\/j.ijpara.2015.01.004_b0095","doi-asserted-by":"crossref","first-page":"4111","DOI":"10.1002\/j.1460-2075.1996.tb00786.x","article-title":"Hch-1, a gene required for normal hatching and normal migration of a neuroblast in C. elegans, encodes a protein related to TOLLOID and BMP-1","volume":"15","author":"Hishida","year":"1996","journal-title":"EMBO J."},{"key":"10.1016\/j.ijpara.2015.01.004_b0100","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1242\/dev.113.2.399","article-title":"\u2018Promoter trapping\u2019 in Caenorhabditis elegans","volume":"113","author":"Hope","year":"1991","journal-title":"Development"},{"key":"10.1016\/j.ijpara.2015.01.004_b0105","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1645\/GE-46R","article-title":"Effect of vaccination with a recombinant fusion protein encoding an astacin like metalloprotease (MTP-1) secreted by host-stimulated Ancylostoma caninum third-stage infective larvae","volume":"89","author":"Hotez","year":"2003","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0110","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0168-9525(99)01857-0","article-title":"Cuticle collagen genes. Expression in Caenorhabditis elegans","volume":"16","author":"Johnstone","year":"2000","journal-title":"Trends Genet"},{"key":"10.1016\/j.ijpara.2015.01.004_b0115","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0166-6851(96)02682-5","article-title":"Cuticular collagen genes from the parasitic nematode Ostertagia circumcincta","volume":"80","author":"Johnstone","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0120","doi-asserted-by":"crossref","first-page":"R88","DOI":"10.1186\/gb-2013-14-8-r88","article-title":"The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery","volume":"14","author":"Laing","year":"2013","journal-title":"Genome Biol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0125","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.vetpar.2008.01.032","article-title":"Vaccination against Haemonchus contortus: performance of native parasite gut membrane glycoproteins in Merino lambs grazing contaminated pasture","volume":"153","author":"LeJambre","year":"2008","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0130","doi-asserted-by":"crossref","first-page":"5127","DOI":"10.1073\/pnas.93.10.5127","article-title":"The C-proteinase that processes procollagens to fibrillar collagens is identical to the protein previously identified as bone morphogenic protein-1","volume":"93","author":"Li","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ijpara.2015.01.004_b0135","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1093\/genetics\/141.3.977","article-title":"Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system","volume":"141","author":"Maduro","year":"1995","journal-title":"Genetics"},{"key":"10.1016\/j.ijpara.2015.01.004_b0140","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.pt.2004.08.002","article-title":"Veterinary anthelmintics: old and new","volume":"20","author":"McKellar","year":"2004","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0145","doi-asserted-by":"crossref","first-page":"4909","DOI":"10.1046\/j.1432-1033.2003.03891.x","article-title":"The astacin protein family in Caenorhabditis elegans","volume":"270","author":"M\u00f6hrlen","year":"2003","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.ijpara.2015.01.004_b0150","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/j.1525-142X.2006.00092.x","article-title":"Evolution of astacin-like metalloproteases in animals and their function in development","volume":"8","author":"M\u00f6hrlen","year":"2006","journal-title":"Evol. Dev."},{"key":"10.1016\/j.ijpara.2015.01.004_b0155","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1534\/genetics.104.027953","article-title":"Gene interactions in Caenorhabditis elegans define DPY-31 as a candidate procollagen C-proteinase and SQT-3\/ROL-4 as its predicted major target","volume":"168","author":"Novelli","year":"2004","journal-title":"Genetics"},{"key":"10.1016\/j.ijpara.2015.01.004_b0160","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1534\/genetics.105.053637","article-title":"The C terminus of collagen SQT-3 has complex and essential functions in nematode collagen assembly","volume":"172","author":"Novelli","year":"2006","journal-title":"Genetics"},{"key":"10.1016\/j.ijpara.2015.01.004_b0165","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/1099-1387(200009)6:9<489::AID-PSC282>3.0.CO;2-K","article-title":"Design and synthesis of acidic dipeptide hydroxamate inhibitors of procollagen C-proteinase","volume":"6","author":"Ovens","year":"2000","journal-title":"J. Peptide Sci."},{"key":"10.1016\/j.ijpara.2015.01.004_b0170","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0065-308X(03)53003-2","article-title":"Enzymes involved in the biogenesis of the nematode cuticle","volume":"53","author":"Page","year":"2003","journal-title":"Adv. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0175","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.ijpddr.2014.05.003","article-title":"Enzymology of the nematode cuticle: a potential drug target?","volume":"4","author":"Page","year":"2014","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"10.1016\/j.ijpara.2015.01.004_b0180","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.vetpar.2012.03.036","article-title":"Anthelmintic resistance in sheep in Europe: a selected review","volume":"189","author":"Papadopoulos","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0185","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1080\/00480169.2006.36709","article-title":"Anthelmintic resistance in New Zealand: a perspective on recent findings and options for the future","volume":"54","author":"Pomroy","year":"2006","journal-title":"N. Z. Vet. J."},{"key":"10.1016\/j.ijpara.2015.01.004_b0190","first-page":"93","article-title":"Creation of transgenic lines using microparticle bombardment methods","volume":"351","author":"Praitis","year":"2006","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0195","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1186\/1756-3305-6-153","article-title":"Impact of gastrointestinal parasitic nematodes of sheep, and the role of advanced molecular tools for exploring epidemiology and drug resistance \u2013 an Australian perspective","volume":"6","author":"Roeber","year":"2013","journal-title":"Parasite Vectors"},{"key":"10.1016\/j.ijpara.2015.01.004_b0200","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.ijpara.2009.10.007","article-title":"Collagen processing and cuticle formation is catalysed by the astacin metalloprotease DPY-31 in free-living and parasitic nematodes","volume":"40","author":"Stepek","year":"2010","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0205","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1017\/S0031182010001113","article-title":"The astacin metalloprotease moulting enzyme NAS-36 is required for normal cuticle ecdysis in free-living and parasitic nematodes","volume":"138","author":"Stepek","year":"2011","journal-title":"Parasitology"},{"key":"10.1016\/j.ijpara.2015.01.004_b0210","first-page":"1","article-title":"Maintenance of C. elegans","author":"Stiernagle","year":"2006","journal-title":"WormBook"},{"key":"10.1016\/j.ijpara.2015.01.004_b0215","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0076-6879(95)48021-8","article-title":"Astacin","volume":"248","author":"St\u00f6cker","year":"1995","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.ijpara.2015.01.004_b0220","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1432-1033.1993.tb17915.x","article-title":"Implications of the three-dimensional structure of astacin for the structure and function of the astacin family of zinc-endopeptidases","volume":"214","author":"St\u00f6cker","year":"1993","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.ijpara.2015.01.004_b0225","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1515\/BC.2004.069","article-title":"Metalloproteases with EGF, CUB, and thrombospondin-1 domains function in molting of Caenorhabditis elegans","volume":"385","author":"Suzuki","year":"2004","journal-title":"Biol. Chem."},{"key":"10.1016\/j.ijpara.2015.01.004_b0230","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1515\/BC.2007.054","article-title":"The protease domain of procollagen C-proteinase (BMP1) lacks substrate selectivity, which is conferred by non-proteolytic domains","volume":"388","author":"Wermter","year":"2007","journal-title":"Biol. Chem."},{"key":"10.1016\/j.ijpara.2015.01.004_b0235","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1093\/genetics\/131.3.609","article-title":"A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites","volume":"131","author":"Williams","year":"1992","journal-title":"Genetics"},{"key":"10.1016\/j.ijpara.2015.01.004_b0240","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1128\/IAI.74.2.961-967.2006","article-title":"Ancylostoma caninum MTP-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migration","volume":"74","author":"Williamson","year":"2006","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijpara.2015.01.004_b0245","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.cvfa.2006.07.006","article-title":"Gastrointestinal nematodes of small ruminants: life cycle, anthelmintics, and diagnosis","volume":"22","author":"Zajac","year":"2006","journal-title":"Vet. Clin. North Am. Food Anim. Pract."}],"container-title":["International Journal for Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751915000387?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751915000387?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T02:34:59Z","timestamp":1651372499000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0020751915000387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":49,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["S0020751915000387"],"URL":"https:\/\/doi.org\/10.1016\/j.ijpara.2015.01.004","relation":{},"ISSN":["0020-7519"],"issn-type":[{"value":"0020-7519","type":"print"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A highly conserved, inhibitable astacin metalloprotease from Teladorsagia circumcincta is required for cuticle formation and nematode development","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijpara.2015.01.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}