{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:22:10Z","timestamp":1790670130147,"version":"4.1.0"},"reference-count":46,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["RSA5780007"],"award-info":[{"award-number":["RSA5780007"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["RSA5880064"],"award-info":[{"award-number":["RSA5880064"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency","doi-asserted-by":"publisher","award":["P-14-50752"],"award-info":[{"award-number":["P-14-50752"]}],"id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency","doi-asserted-by":"publisher","award":["P-13-00832"],"award-info":[{"award-number":["P-13-00832"]}],"id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004192","name":"National Science and Technology Development Agency","doi-asserted-by":"publisher","award":["P-14-50883"],"award-info":[{"award-number":["P-14-50883"]}],"id":[{"id":"10.13039\/501100004192","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cluster and Program Management"},{"DOI":"10.13039\/100006641","name":"United Nations Children\u2019s Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006641","id-type":"DOI","asserted-by":"crossref"}]},{"name":"United Nations Development Programme"},{"DOI":"10.13039\/100004421","name":"World Bank","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"publisher","award":["A50098"],"award-info":[{"award-number":["A50098"]}],"id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["55005512"],"award-info":[{"award-number":["55005512"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["55005915"],"award-info":[{"award-number":["55005915"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"name":"WellcomeTrust African Institutions Initiative"},{"name":"Institute for Infectious Diseases of Poverty","award":["WT087536MA"],"award-info":[{"award-number":["WT087536MA"]}]},{"name":"Thailand National Center for Genetic Engineering and Biotechnology"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology"],"published-print":{"date-parts":[[2016,7]]},"DOI":"10.1016\/j.ijpara.2016.04.002","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T02:02:27Z","timestamp":1462154547000},"page":"527-535","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"title":["Identifying antimalarial compounds targeting dihydrofolate reductase-thymidylate synthase (DHFR-TS) by chemogenomic profiling"],"prefix":"10.1016","volume":"46","author":[{"given":"Aiyada","family":"Aroonsri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olugbenga","family":"Akinola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navaporn","family":"Posayapisit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warangkhana","family":"Songsungthong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chairat","family":"Uthaipibull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumalee","family":"Kamchonwongpaisan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace O.","family":"Gbotosho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongyuth","family":"Yuthavong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3913-3057","authenticated-orcid":false,"given":"Philip J.","family":"Shaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijpara.2016.04.002_b0005","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.fct.2004.11.006","article-title":"Glucosamine effects in humans: a review of effects on glucose metabolism, side effects, safety considerations and efficacy","volume":"43","author":"Anderson","year":"2005","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0010","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature12876","article-title":"A molecular marker of artemisinin-resistant Plasmodium falciparum malaria","volume":"505","author":"Ariey","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.ijpara.2016.04.002_b0015","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/1471-2180-9-83","article-title":"PiggyBac is an effective tool for functional analysis of the Plasmodium falciparum genome","volume":"9","author":"Balu","year":"2009","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0020","doi-asserted-by":"crossref","first-page":"e0149296","DOI":"10.1371\/journal.pone.0149296","article-title":"Contrasting inducible knockdown of the auxiliary PTEX component PTEX88 in P. falciparum and P. berghei unmasks a role in parasite virulence","volume":"11","author":"Chisholm","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.ijpara.2016.04.002_b0025","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nrmicro3450","article-title":"Advances in molecular genetic systems in malaria","volume":"13","author":"de Koning-Ward","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0030","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nature13555","article-title":"PTEX is an essential nexus for protein export in malaria parasites","volume":"511","author":"Elsworth","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.ijpara.2016.04.002_b0035","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1006\/abio.1993.1346","article-title":"A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli","volume":"212","author":"Feliciello","year":"1993","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ijpara.2016.04.002_b0040","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1124\/mol.54.6.1140","article-title":"Cycloguanil and its parent compound proguanil demonstrate distinct activities against Plasmodium falciparum malaria parasites transformed with human dihydrofolate reductase","volume":"54","author":"Fidock","year":"1998","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0045","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/nrmicro3138","article-title":"Antimalarial drug discovery\u2014approaches and progress towards new medicines","volume":"11","author":"Flannery","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0050","doi-asserted-by":"crossref","first-page":"3014","DOI":"10.1073\/pnas.87.8.3014","article-title":"Amino acids in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum involved in cycloguanil resistance differ from those involved in pyrimethamine resistance","volume":"87","author":"Foote","year":"1990","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ijpara.2016.04.002_b0055","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.molbiopara.2004.04.007","article-title":"A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle","volume":"137","author":"Franke-Fayard","year":"2004","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0060","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/nature09107","article-title":"Thousands of chemical starting points for antimalarial lead identification","volume":"465","author":"Gamo","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.ijpara.2016.04.002_b0065","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1038\/nbt.2925","article-title":"Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system","volume":"32","author":"Ghorbal","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0070","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1038\/6791","article-title":"Genomic profiling of drug sensitivities via induced haploinsufficiency","volume":"21","author":"Giaever","year":"1999","journal-title":"Nat. Genet."},{"key":"10.1016\/j.ijpara.2016.04.002_b0075","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1073\/pnas.0307490100","article-title":"Chemogenomic profiling: identifying the functional interactions of small molecules in yeast","volume":"101","author":"Giaever","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ijpara.2016.04.002_b0080","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nature09099","article-title":"Chemical genetics of Plasmodium falciparum","volume":"465","author":"Guiguemde","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.ijpara.2016.04.002_b0085","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.chembiol.2012.01.004","article-title":"Global phenotypic screening for antimalarials","volume":"19","author":"Guiguemde","year":"2012","journal-title":"Chem. Biol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0090","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1038\/nprot.2006.53","article-title":"High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei","volume":"1","author":"Janse","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ijpara.2016.04.002_b0095","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.molbiopara.2005.09.007","article-title":"High efficiency transfection of Plasmodium berghei facilitates novel selection procedures","volume":"145","author":"Janse","year":"2006","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0100","doi-asserted-by":"crossref","first-page":"e57","DOI":"10.1371\/journal.ppat.0020057","article-title":"A systematic map of genetic variation in Plasmodium falciparum","volume":"2","author":"Kidgell","year":"2006","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ijpara.2016.04.002_b0105","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1093\/molbev\/msu140","article-title":"Origin of robustness in generating drug-resistant malaria parasites","volume":"31","author":"Kumpornsin","year":"2014","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0110","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1111\/mmi.13201","article-title":"A repeat sequence domain of the ring-exported protein-1 of Plasmodium falciparum controls export machinery architecture and virulence protein trafficking: A REX1 domain controls Maurer\u2019s cleft architecture","volume":"98","author":"McHugh","year":"2015","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0115","doi-asserted-by":"crossref","first-page":"e1000243","DOI":"10.1371\/journal.pgen.1000243","article-title":"Adaptive copy number evolution in malaria parasites","volume":"4","author":"Nair","year":"2008","journal-title":"PLoS Genet."},{"key":"10.1016\/j.ijpara.2016.04.002_b0120","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1038\/nchembio.1963","article-title":"Functional genomics to uncover drug mechanism of action","volume":"11","author":"Nijman","year":"2015","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0125","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.chom.2015.05.018","article-title":"Conditional degradation of Plasmodium calcineurin reveals functions in parasite colonization of both host and vector","volume":"18","author":"Philip","year":"2015","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ijpara.2016.04.002_b0130","doi-asserted-by":"crossref","first-page":"3649","DOI":"10.1021\/jm8001026","article-title":"Triazolopyrimidine-based dihydroorotate dehydrogenase inhibitors with potent and selective activity against the malaria parasite Plasmodium falciparum","volume":"51","author":"Phillips","year":"2008","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpara.2016.04.002_b0135","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.chom.2012.10.016","article-title":"A tetracycline-repressible transactivator system to study essential genes in malaria parasites","volume":"12","author":"Pino","year":"2012","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ijpara.2016.04.002_b0140","doi-asserted-by":"crossref","first-page":"9059","DOI":"10.1073\/pnas.0802982105","article-title":"In silico activity profiling reveals the mechanism of action of antimalarials discovered in a high-throughput screen","volume":"105","author":"Plouffe","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ijpara.2016.04.002_b0145","doi-asserted-by":"crossref","first-page":"15930","DOI":"10.1038\/srep15930","article-title":"Chemogenomic profiling of Plasmodium falciparum as a tool to aid antimalarial drug discovery","volume":"5","author":"Pradhan","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijpara.2016.04.002_b0150","doi-asserted-by":"crossref","first-page":"e73783","DOI":"10.1371\/journal.pone.0073783","article-title":"Inducible knockdown of Plasmodium gene expression using the glmS ribozyme","volume":"8","author":"Prommana","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ijpara.2016.04.002_b0155","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v012.i05","article-title":"Bioassay analysis using R","volume":"12","author":"Ritz","year":"2005","journal-title":"J. Stat. Softw."},{"key":"10.1016\/j.ijpara.2016.04.002_b0160","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1126\/science.1193225","article-title":"Spiroindolones, a potent compound class for the treatment of malaria","volume":"329","author":"Rottmann","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.ijpara.2016.04.002_b0165","doi-asserted-by":"crossref","first-page":"e1001897","DOI":"10.1371\/journal.pbio.1001897","article-title":"Inhibition of plasmepsin V activity demonstrates its essential role in protein export, PfEMP1 display, and survival of malaria parasites","volume":"12","author":"Sleebs","year":"2014","journal-title":"PLoS Biol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0170","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1128\/AAC.48.5.1803-1806.2004","article-title":"Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening","volume":"48","author":"Smilkstein","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpara.2016.04.002_b0175","doi-asserted-by":"crossref","DOI":"10.1186\/s12916-015-0384-6","article-title":"Malaria eradication and elimination: views on how to translate a vision into reality","volume":"13","author":"Tanner","year":"2015","journal-title":"BMC Med."},{"key":"10.1016\/j.ijpara.2016.04.002_b0180","doi-asserted-by":"crossref","first-page":"3807","DOI":"10.1093\/emboj\/17.14.3807","article-title":"Allelic exchange at the endogenous genomic locus in Plasmodium falciparum proves the role of dihydropteroate synthase in sulfadoxine-resistant malaria","volume":"17","author":"Triglia","year":"1998","journal-title":"EMBO J."},{"key":"10.1016\/j.ijpara.2016.04.002_b0185","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0166-6851(99)00175-9","article-title":"The rodent malaria parasite Plasmodium berghei does not contain a typical O-type small subunit ribosomal RNA gene","volume":"105","author":"van Spaendonk","year":"2000","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0190","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/nmeth.3063","article-title":"Efficient CRISPR-Cas9\u2013mediated genome editing in Plasmodium falciparum","volume":"11","author":"Wagner","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ijpara.2016.04.002_b0195","first-page":"28","article-title":"The generalization of \u201cStudent\u2019s\u201d problem when several different population variances are involved","volume":"34","author":"Welch","year":"1947","journal-title":"Biometrika"},{"key":"10.1016\/j.ijpara.2016.04.002_b0200","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/nature02362","article-title":"Control of gene expression by a natural metabolite-responsive ribozyme","volume":"428","author":"Winkler","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.ijpara.2016.04.002_b0205","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.178830","article-title":"Haemoglobin degradation underpins the sensitivity of early ring stage Plasmodium falciparum to artemisinins","author":"Xie","year":"2015","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.ijpara.2016.04.002_b0210","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.chom.2008.11.001","article-title":"The fatty acid biosynthesis enzyme FabI plays a key role in the development of liver-stage malarial parasites","volume":"4","author":"Yu","year":"2008","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ijpara.2016.04.002_b0215","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.exppara.2014.03.010","article-title":"Refrigeration provides a simple means to synchronize in vitro cultures of Plasmodium falciparum","volume":"140","author":"Yuan","year":"2014","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0220","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1286-4579(01)01525-8","article-title":"Basis for antifolate action and resistance in malaria","volume":"4","author":"Yuthavong","year":"2002","journal-title":"Microbes Infect."},{"key":"10.1016\/j.ijpara.2016.04.002_b0225","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nsb921","article-title":"Insights into antifolate resistance from malarial DHFR-TS structures","volume":"10","author":"Yuvaniyama","year":"2003","journal-title":"Nat. Struct. Biol."},{"key":"10.1016\/j.ijpara.2016.04.002_b0230","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01414-14","article-title":"Efficient editing of malaria parasite genome using the CRISPR\/Cas9 System","volume":"5","author":"Zhang","year":"2014","journal-title":"mBio"}],"container-title":["International Journal for Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751916300455?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751916300455?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,6,19]],"date-time":"2022-06-19T11:03:17Z","timestamp":1655636597000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0020751916300455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":46,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["S0020751916300455"],"URL":"https:\/\/doi.org\/10.1016\/j.ijpara.2016.04.002","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726338004.793523856","asserted-by":"object"}]},"ISSN":["0020-7519"],"issn-type":[{"value":"0020-7519","type":"print"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identifying antimalarial compounds targeting dihydrofolate reductase-thymidylate synthase (DHFR-TS) by chemogenomic profiling","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijpara.2016.04.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}