{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T11:21:08Z","timestamp":1786533668716,"version":"build-2736575974"},"reference-count":50,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["14-33693S"],"award-info":[{"award-number":["14-33693S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["13-11043S"],"award-info":[{"award-number":["13-11043S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["GACR"],"award-info":[{"award-number":["GACR"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.ijpara.2017.08.011","type":"journal-article","created":{"date-parts":[[2017,11,4]],"date-time":"2017-11-04T20:16:49Z","timestamp":1509826609000},"page":"167-178","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Multiple legumain isoenzymes in ticks"],"prefix":"10.1016","volume":"48","author":[{"given":"David","family":"Hartmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radek","family":"\u0160\u00edma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitka","family":"Konvi\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Perner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Kop\u00e1\u010dek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Sojka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijpara.2017.08.011_b0005","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.ibmb.2007.04.010","article-title":"Characterization of asparaginyl endopeptidase, legumain induced by blood feeding in the ixodid tick Haemaphysalis longicornis","volume":"37","author":"Abdul Alim","year":"2007","journal-title":"Insect. Biochem. Mol. Biol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0010","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.molbiopara.2007.07.006","article-title":"Comparative molecular analysis of two asparaginyl endopeptidases and encoding genes from Fasciola gigantica","volume":"156","author":"Adisakwattana","year":"2007","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0015","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.ijpara.2008.06.012","article-title":"Legumains from the hard tick Haemaphysalis longicornis play modulatory roles in blood feeding and gut cellular remodelling and impact on embryogenesis","volume":"39","author":"Alim","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0020","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.jinsphys.2007.12.006","article-title":"HlLgm2, a member of asparaginyl endopeptidases\/legumains in the midgut of the ixodid tick Haemaphysalis longicornis, is involved in blood-meal digestion","volume":"54","author":"Alim","year":"2008","journal-title":"J. Insect. Physiol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0025","doi-asserted-by":"crossref","first-page":"19195","DOI":"10.1074\/jbc.274.27.19195","article-title":"Inhibition of mammalian legumain by some cystatins is due to a novel second reactive site","volume":"274","author":"Alvarez-Fernandez","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0030","doi-asserted-by":"crossref","first-page":"4958","DOI":"10.1021\/jm025581c","article-title":"Aza-peptide epoxides: a new class of inhibitors selective for clan CD cysteine proteases","volume":"45","author":"Asgian","year":"2002","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0035","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1016\/j.biochi.2012.11.002","article-title":"Autoactivation of prolegumain is accelerated by glycosaminoglycans","volume":"95","author":"Berven","year":"2013","journal-title":"Biochimie"},{"key":"10.1016\/j.ijpara.2017.08.011_b0040","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/S0014-5793(99)01798-6","article-title":"Identification of a cDNA encoding an active asparaginyl endopeptidase of Schistosoma mansoni and its expression in Pichia pastoris","volume":"466","author":"Caffrey","year":"2000","journal-title":"FEBS Lett."},{"key":"10.1016\/j.ijpara.2017.08.011_b0045","doi-asserted-by":"crossref","first-page":"8090","DOI":"10.1074\/jbc.272.12.8090","article-title":"Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase","volume":"272","author":"Chen","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0050","doi-asserted-by":"crossref","first-page":"10940","DOI":"10.1073\/pnas.1300686110","article-title":"Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation","volume":"110","author":"Dall","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ijpara.2017.08.011_b0055","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.biochi.2015.09.022","article-title":"Structure and function of legumain in health and disease","volume":"122","author":"Dall","year":"2016","journal-title":"Biochimie"},{"key":"10.1016\/j.ijpara.2017.08.011_b0060","doi-asserted-by":"crossref","first-page":"2917","DOI":"10.1002\/anie.201409135","article-title":"Structure and mechanism of an aspartimide-dependent peptide ligase in human legumain","volume":"54","author":"Dall","year":"2015","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.ijpara.2017.08.011_b0065","doi-asserted-by":"crossref","first-page":"39316","DOI":"10.1074\/jbc.M607128200","article-title":"A multienzyme network functions in intestinal protein digestion by a platyhelminth parasite","volume":"281","author":"Delcroix","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0070","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1021\/ja307083b","article-title":"Functional imaging of legumain in cancer using a new quenched activity-based probe","volume":"135","author":"Edgington","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ijpara.2017.08.011_b0075","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1021\/jm049938j","article-title":"Aza-peptide Michael acceptors: a new class of inhibitors specific for caspases and other clan CD cysteine proteases","volume":"47","author":"Ekici","year":"2004","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0080","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.cbpb.2007.07.009","article-title":"A cysteine endopeptidase from tick Rhipicephalus (Boophilus) microplus larvae with vitellin digestion activity","volume":"148","author":"Estrela","year":"2007","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0085","doi-asserted-by":"crossref","first-page":"2816","DOI":"10.1021\/jm701311r","article-title":"Aza-peptidyl Michael acceptors. A new class of potent and selective inhibitors of asparaginyl endopeptidases (legumains) from evolutionarily diverse pathogens","volume":"51","author":"Gotz","year":"2008","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0090","doi-asserted-by":"crossref","first-page":"10507","DOI":"10.1038\/ncomms10507","article-title":"Genomic insights into the Ixodes scapularis tick vector of Lyme disease","volume":"7","author":"Gulia-Nuss","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ijpara.2017.08.011_b0095","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1016\/j.vaccine.2010.02.008","article-title":"Characterization of ferritin 2 for the control of tick infestations","volume":"28","author":"Hajdusek","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.ijpara.2017.08.011_b0100","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1073\/pnas.0807961106","article-title":"Knockdown of proteins involved in iron metabolism limits tick reproduction and development","volume":"106","author":"Hajdusek","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ijpara.2017.08.011_b0105","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/j.chembiol.2009.09.009","article-title":"Hemoglobin digestion in blood-feeding ticks: mapping a multipeptidase pathway by functional proteomics","volume":"16","author":"Horn","year":"2009","journal-title":"Chem. Biol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0110","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ijpara.2017.08.011_b0115","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.pt.2007.07.010","article-title":"In vitro feeding assays for hard ticks","volume":"23","author":"Krober","year":"2007","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0120","doi-asserted-by":"crossref","first-page":"3093","DOI":"10.1242\/jeb.01749","article-title":"Tracing heme in a living cell: hemoglobin degradation and heme traffic in digest cells of the cattle tick Boophilus microplus","volume":"208","author":"Lara","year":"2005","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0125","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1016\/j.bmcl.2011.12.079","article-title":"Synthesis and evaluation of aza-peptidyl inhibitors of the lysosomal asparaginyl endopeptidase, legumain","volume":"22","author":"Lee","year":"2012","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.ijpara.2017.08.011_b0130","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.gene.2004.03.002","article-title":"Two novel asparaginyl endopeptidase-like cysteine proteinases from the protist Trichomonas vaginalis: their evolutionary relationship within the clan CD cysteine proteinases","volume":"335","author":"Leon-Felix","year":"2004","journal-title":"Gene"},{"key":"10.1016\/j.ijpara.2017.08.011_b0135","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0092-8674(01)00267-7","article-title":"Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae","volume":"104","author":"Levashina","year":"2001","journal-title":"Cell"},{"key":"10.1016\/j.ijpara.2017.08.011_b0140","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1021\/mp200434n","article-title":"Targeting cell surface alpha(v)beta(3) integrin increases therapeutic efficacies of a legumain protease-activated auristatin prodrug","volume":"9","author":"Liu","year":"2012","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0145","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1038\/25379","article-title":"An asparaginyl endopeptidase processes a microbial antigen for class II MHC presentation","volume":"396","author":"Manoury","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.ijpara.2017.08.011_b0150","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1038\/nchembio.1586","article-title":"Butelase 1 is an Asx-specific ligase enabling peptide macrocyclization and synthesis","volume":"10","author":"Nguyen","year":"2014","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0155","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1515\/BC.2002.133","article-title":"Inhibition of mammalian legumain by Michael acceptors and AzaAsn-halomethylketones","volume":"383","author":"Niestroj","year":"2002","journal-title":"Biol. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0160","doi-asserted-by":"crossref","first-page":"7192","DOI":"10.1021\/jm900849h","article-title":"Aza-peptidyl Michael acceptor and epoxide inhibitors\u2013potent and selective inhibitors of Schistosoma mansoni and Ixodes ricinus legumains (asparaginyl endopeptidases)","volume":"52","author":"Ovat","year":"2009","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0165","doi-asserted-by":"crossref","first-page":"36695","DOI":"10.1038\/srep36695","article-title":"RNA-seq analyses of the midgut from blood- and serum-fed Ixodes ricinus ticks","volume":"6","author":"Perner","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ijpara.2017.08.011_b0170","first-page":"5","article-title":"Acquisition of exogenous haem is essential for tick reproduction","author":"Perner","year":"2016","journal-title":"Elife"},{"key":"10.1016\/j.ijpara.2017.08.011_b0175","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"SignalP 4.0: discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen","year":"2011","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ijpara.2017.08.011_b0180","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2013a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.ijpara.2017.08.011_b0185","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.jmb.2004.04.063","article-title":"Inactive enzyme-homologues find new function in regulatory processes","volume":"340","author":"Pils","year":"2004","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0190","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1111\/mmi.12232","article-title":"From technology to biology: a malaria genetic toolbox for the functional dissection of essential genes","volume":"88","author":"Pino","year":"2013","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0195","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/j.chembiol.2016.05.020","article-title":"Counter selection substrate library strategy for developing specific protease substrates and probes","volume":"23","author":"Poreba","year":"2016","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0200","doi-asserted-by":"crossref","first-page":"D227","DOI":"10.1093\/nar\/gkp971","article-title":"MEROPS: the peptidase database","volume":"38","author":"Rawlings","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ijpara.2017.08.011_b0205","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1038\/sj.cdd.4401247","article-title":"Inhibition of papain-like cysteine proteases and legumain by caspase-specific inhibitors: when reaction mechanism is more important than specificity","volume":"10","author":"Rozman-Pungercar","year":"2003","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.ijpara.2017.08.011_b0210","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0166-6851(03)00194-4","article-title":"Functional expression and characterization of Schistosoma mansoni cathepsin B and its trans-activation by an endogenous asparaginyl endopeptidase","volume":"131","author":"Sajid","year":"2003","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0215","doi-asserted-by":"crossref","first-page":"e43011","DOI":"10.1371\/journal.pone.0043011","article-title":"Gene pyramiding of peptidase inhibitors enhances plant resistance to the spider mite Tetranychus urticae","volume":"7","author":"Santamaria","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ijpara.2017.08.011_b0220","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.pt.2013.04.002","article-title":"New insights into the machinery of blood digestion by ticks","volume":"29","author":"Sojka","year":"2013","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0225","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1756-3305-1-7","article-title":"Profiling of proteolytic enzymes in the gut of the tick Ixodes ricinus reveals an evolutionarily conserved network of aspartic and cysteine peptidases","volume":"1","author":"Sojka","year":"2008","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.ijpara.2017.08.011_b0230","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.ijpara.2006.12.020","article-title":"IrAE: an asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus","volume":"37","author":"Sojka","year":"2007","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0235","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1016\/j.pt.2016.05.015","article-title":"Parasite Cathepsin D-Like Peptidases and Their Relevance as Therapeutic Targets","volume":"32","author":"Sojka","year":"2016","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0240","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.ttbdis.2015.12.014","article-title":"Multienzyme degradation of host serum albumin in ticks","volume":"7","author":"Sojka","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"10.1016\/j.ijpara.2017.08.011_b0245","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S1471-4922(03)00189-2","article-title":"Digestive proteases of blood-feeding nematodes","volume":"19","author":"Williamson","year":"2003","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpara.2017.08.011_b0250","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/cr.2014.4","article-title":"Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage","volume":"24","author":"Zhao","year":"2014","journal-title":"Cell Res."}],"container-title":["International Journal for Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751917303016?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751917303016?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:18:30Z","timestamp":1778635110000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0020751917303016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["S0020751917303016"],"URL":"https:\/\/doi.org\/10.1016\/j.ijpara.2017.08.011","relation":{},"ISSN":["0020-7519"],"issn-type":[{"value":"0020-7519","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multiple legumain isoenzymes in ticks","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijpara.2017.08.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}