{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T16:32:39Z","timestamp":1793464359836,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"10-11","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.ijpara.2020.04.006","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T20:50:14Z","timestamp":1593031814000},"page":"851-857","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["In vivo cultivation of tuna blood fluke Cardicola orientalis in terebellid intermediate hosts"],"prefix":"10.1016","volume":"50","author":[{"given":"Sho","family":"Shirakashi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoya","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanami","family":"Asai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Honryo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijpara.2020.04.006_b0005","series-title":"Advances in Tuna Aquaculture","first-page":"253","article-title":"Diseases in tuna aquaculture","author":"Balli","year":"2015"},{"key":"10.1016\/j.ijpara.2020.04.006_b0010","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1046\/j.1365-2761.2001.00289.x","article-title":"Pathological findings in southern bluefin tuna, Thunnus maccoyii (Castelnau), infected with Cardicola forsteri (Cribb, Daintith & Munday, 2000) (Digenea : Sanguinicolidae), a blood fluke","volume":"24","author":"Colquitt","year":"2001","journal-title":"J. Fish Dis."},{"key":"10.1016\/j.ijpara.2020.04.006_b0015","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.ijpara.2011.03.011","article-title":"The life cycle of Cardicola forsteri (Trematoda: Aporocotylidae), a pathogen of ranched southern bluefin tuna, Thunnus maccoyi","volume":"41","author":"Cribb","year":"2011","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0020","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/j.ijpara.2017.05.008","article-title":"Evidence that blood flukes (Trematoda: Aporocotylidae) of chondrichthyans infect bivalves as intermediate hosts: indications of an ancient diversification of the Schistosomatoidea","volume":"47","author":"Cribb","year":"2017","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0025","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1177\/0300985810388524","article-title":"Case-control study of epidemic mortality and Cardicola forsteri-associated disease in farmed Southern bluefin tuna (Thunnus maccoyii) of South Australia","volume":"48","author":"Dennis","year":"2011","journal-title":"Vet. Pathol."},{"issue":"131","key":"10.1016\/j.ijpara.2020.04.006_b0030","article-title":"Mass isolation and in vitro cultivation of intramolluscan stages of the human blood fluke Schistosoma mansoni","volume":"14","author":"Dinguirard","year":"2018","journal-title":"J. Vis. Expt."},{"key":"10.1016\/j.ijpara.2020.04.006_b0035","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0020-7519(71)90046-4","article-title":"The potential number of redial generations in echinostomatids (Trematoda)","volume":"1","author":"D\u00f6nges","year":"1971","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0040","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.ijpara.2019.05.004","article-title":"Failure of in vitro-cultured schistosomes to produce eggs: how does the parasite meet its needs for host-derived cytokines such as TGF-beta?","volume":"49","author":"Doenhoff","year":"2019","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.ijpara.2020.04.006_b0140","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006590","article-title":"A novel cell-free method to culture Schistosoma mansoni from cercariae to juvenile worm stages for in vitro drug testing","volume":"13","author":"Frahm","year":"2019","journal-title":"PLOS Negl. Trop. Dis."},{"key":"10.1016\/j.ijpara.2020.04.006_b0045","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.aquaculture.2013.03.013","article-title":"Praziquantel treatment against Cardicola blood flukes: determination of the minimal effective dose and pharmacokinetics in juvenile Pacific bluefin tuna","volume":"402","author":"Ishimaru","year":"2013","journal-title":"Aquaculture"},{"key":"10.1016\/j.ijpara.2020.04.006_b0050","first-page":"177","article-title":"Demonstration of several sporocysts generations as a normal pattern of reproduction of Schistosoma mansoni","volume":"37","author":"Jourdane","year":"1980","journal-title":"Acta Trop."},{"key":"10.1016\/j.ijpara.2020.04.006_b0055","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1080\/00785326.1982.10426582","article-title":"The redia, cercaria and early stages of Aporocotyle simplex Odhner, 1900 (Sanguinicolidae) \u2013 a digenetic trematode which has a polychaete annelid as the only intermediate host","volume":"21","author":"K\u00f8ie","year":"1982","journal-title":"Ophelia"},{"key":"10.1016\/j.ijpara.2020.04.006_b0060","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0023705","article-title":"Moving cages further offshore: effects on Southern bluefin tuna, T. maccoyii, parasites, health and performance","volume":"6","author":"Kirchhoff","year":"2011","journal-title":"PLoS ONE"},{"key":"10.1016\/j.ijpara.2020.04.006_b0065","series-title":"Fish Parasites: Pathobiology and Protection","first-page":"270","article-title":"Sanguinicola inermis and related species","author":"Kirk","year":"2012"},{"key":"10.1016\/j.ijpara.2020.04.006_b0070","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.exppara.2011.07.009","article-title":"In vitro culture of marine trematodes from their snail first intermediate host","volume":"129","author":"Lloyd","year":"2011","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0075","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1017\/S0031182014000808","article-title":"Diseases of cultured marine fishes caused by Platyhelminthes (Monogenea, Digenea, Cestoda)","volume":"142","author":"Ogawa","year":"2015","journal-title":"Parasitology"},{"key":"10.1016\/j.ijpara.2020.04.006_b0080","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3147\/jsfp.21.15","article-title":"Two new species of Paradeontacylix MCINTOSH, 1934 (Trematoda:Sanguinicolidae) from the vascular system of a cultured marine fish, Seriola purpurascens","volume":"21","author":"Ogawa","year":"1986","journal-title":"Fish Pathol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0085","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1016\/j.parint.2016.10.016","article-title":"Developmental stages of fish blood flukes, Cardicola forsteri and Cardicola opisthorchis (Trematoda: Aporocotylidae), in their polychaete intermediate hosts collected at Pacific bluefin tuna culture sites in Japan","volume":"66","author":"Ogawa","year":"2017","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ijpara.2020.04.006_b0090","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s11230-015-9568-x","article-title":"Morphological characterisation and identification of four species of Cardicola Short, 1953 (Trematoda: Aporocotylidae) infecting the Atlantic bluefin tuna Thunnus thynnus (L.) in the Mediterranean Sea","volume":"91","author":"Palacios-Abella","year":"2015","journal-title":"Syst. Parasitol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0095","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.parint.2011.10.002","article-title":"Morphology and distribution of blood fluke eggs and associated pathology in the gills of cultured Pacific bluefin tuna, Thunnus orientalis","volume":"61","author":"Shirakashi","year":"2012","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ijpara.2020.04.006_b0100","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.aquaculture.2011.10.035","article-title":"Oral treatment of praziquantel as an effective control measure against blood fluke infection in Pacific bluefin tuna (Thunnus orientalis)","volume":"326","author":"Shirakashi","year":"2012","journal-title":"Aquaculture"},{"key":"10.1016\/j.ijpara.2020.04.006_b0145","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.parint.2015.11.003","article-title":"Discovery of intermediate hosts for two species of blood flukes Cardicola orientalis and Cardicola forsteri (Trematoda: Aporocotylidae) infecting Pacific bluefin tuna in Japan","volume":"65","author":"Shirakashi","year":"2016","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ijpara.2020.04.006_b0105","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1645\/17-70","article-title":"Spatial and temporal changes in the distribution of blood fluke infection in Nicolea gracilibranchis (Polychaeta: Terebellidae), the intermediate host for Cardicola orientalis (Digenea: Aporocotylidae), at a tuna farming site in Japan","volume":"103","author":"Shirakashi","year":"2017","journal-title":"J. Parasitol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0110","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.parint.2017.10.012","article-title":"Blood flukes Cardicola parvus and C. laruei (Trematoda: Aporocotylidae): life cycles and cryptic infection in spotted seatrout, Cynoscion nebulosus (Teleost: Sciaenidae)","volume":"67","author":"Siegel","year":"2018","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ijpara.2020.04.006_b0115","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/j.parint.2016.09.014","article-title":"Transplantation of Cardicola opisthorchis (Trematoda: Aporocotylidae) sporocysts into the intermediate host, Terebella sp (Polychaeta: Terebellidae)","volume":"66","author":"Sugihara","year":"2017","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ijpara.2020.04.006_b0120","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3147\/jsfp.50.105","article-title":"Occurrence of the bluefin tuna blood fluke Cardicola opisthorchis in the intermediate host Terebella sp","volume":"50","author":"Sugihara","year":"2015","journal-title":"Fish Pathol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0125","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.parint.2013.11.010","article-title":"Larval stages of the bluefin tuna blood fluke Cardicola opisthorchis (Trematoda: Aporocotylidae) found from Terebella sp. (Polychaeta: Terebellidae)","volume":"63","author":"Sugihara","year":"2014","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.ijpara.2020.04.006_b0130","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000254","article-title":"Systematically improved in vitro culture conditions reveal new insights into the reproductive biology of the human parasite Schistosoma mansoni","volume":"17","author":"Wang","year":"2019","journal-title":"PLoS Biol."},{"key":"10.1016\/j.ijpara.2020.04.006_b0135","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1016\/j.biotechadv.2013.09.003","article-title":"In vitro cultivation of Schistosoma japonicum-parasites and cells","volume":"31","author":"Ye","year":"2013","journal-title":"Biotechnol. Adv."}],"container-title":["International Journal for Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751920301533?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751920301533?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T14:06:29Z","timestamp":1758117989000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0020751920301533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":29,"journal-issue":{"issue":"10-11","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["S0020751920301533"],"URL":"https:\/\/doi.org\/10.1016\/j.ijpara.2020.04.006","relation":{},"ISSN":["0020-7519"],"issn-type":[{"value":"0020-7519","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In vivo cultivation of tuna blood fluke Cardicola orientalis in terebellid intermediate hosts","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijpara.2020.04.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}