{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T20:22:40Z","timestamp":1780518160019,"version":"3.54.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T00:00:00Z","timestamp":1684108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["1R01-HD099072"],"award-info":[{"award-number":["1R01-HD099072"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI056189"],"award-info":[{"award-number":["R01-AI056189"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI150866"],"award-info":[{"award-number":["R01-AI150866"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21-AI149037"],"award-info":[{"award-number":["R21-AI149037"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology"],"published-print":{"date-parts":[[2023,7]]},"DOI":"10.1016\/j.ijpara.2023.05.001","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T21:24:06Z","timestamp":1684272246000},"page":"451-458","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["An efficient method for viable cryopreservation and recovery of hookworms and other gastrointestinal nematodes in the laboratory"],"prefix":"10.1016","volume":"53","author":[{"given":"Hanchen","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gazzola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9741-3834","authenticated-orcid":false,"given":"Raffi V.","family":"Aroian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.ijpara.2023.05.001_b0005","doi-asserted-by":"crossref","first-page":"735","DOI":"10.2307\/3283058","article-title":"Radiolabeling of Infective Third-Stage Larvae of Strongyloides stercoralis by Feeding [75 Se]Selenomethionine-Labeled Escherichia coli to First- and Second-Stage Larvae","volume":"75","author":"Aikens","year":"1989","journal-title":"J. Parasitol."},{"issue":"9","key":"10.1016\/j.ijpara.2023.05.001_b0010","doi-asserted-by":"crossref","first-page":"e0004922","DOI":"10.1371\/journal.pntd.0004922","article-title":"The Global Economic and Health Burden of Human Hookworm Infection","volume":"10","author":"Bartsch","year":"2016","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"12","key":"10.1016\/j.ijpara.2023.05.001_b0015","doi-asserted-by":"crossref","first-page":"e0009908","DOI":"10.1371\/journal.pntd.0009908","article-title":"Experimental human hookworm infection: a narrative historical review","volume":"15","author":"Chapman","year":"2021","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"2","key":"10.1016\/j.ijpara.2023.05.001_b0020","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1007\/s00436-014-4238-5","article-title":"Storage of gastrointestinal nematode infective larvae for species preservation and experimental infections","volume":"114","author":"Chylinski","year":"2015","journal-title":"Parasitol. Res."},{"issue":"1","key":"10.1016\/j.ijpara.2023.05.001_b0025","doi-asserted-by":"crossref","first-page":"44","DOI":"10.4269\/ajtmh.2003.68.1.0680044","article-title":"Short report: Ancylostoma ceylanicum: exsheathment is not required for successful cryopreservation of third stage hookworm larvae","volume":"68","author":"Duarte","year":"2003","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.ijpara.2023.05.001_b0030","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1186\/1471-2148-10-264","article-title":"The landscape of human genes involved in the immune response to parasitic worms","volume":"10","author":"Fumagalli","year":"2010","journal-title":"BMC Evol. Biol."},{"issue":"11","key":"10.1016\/j.ijpara.2023.05.001_b0035","doi-asserted-by":"crossref","first-page":"e1002355","DOI":"10.1371\/journal.pgen.1002355","article-title":"Signatures of environmental genetic adaptation pinpoint pathogens as the main selective pressure through human evolution","volume":"7","author":"Fumagalli","year":"2011","journal-title":"PLoS Genet."},{"key":"10.1016\/j.ijpara.2023.05.001_b0045","doi-asserted-by":"crossref","first-page":"105214","DOI":"10.1016\/j.actatropica.2019.105214","article-title":"Human hookworm infection: is effective control possible? A review of hookworm control efforts and future directions","volume":"201","author":"Haldeman","year":"2020","journal-title":"Acta Trop."},{"issue":"14","key":"10.1016\/j.ijpara.2023.05.001_b0050","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2023185118","article-title":"Twenty-Year economic impacts of deworming","volume":"118","author":"Hamory","year":"2021","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"10.1016\/j.ijpara.2023.05.001_b0055","first-page":"140","article-title":"Long Term Storage of Hookworm Infective Larvae in Buffered Saline Solution Maintains Larval Responsiveness to Host Signals","volume":"58","author":"Hawdon","year":"1991","journal-title":"J. Helminthol. Soc. Wash."},{"issue":"3","key":"10.1016\/j.ijpara.2023.05.001_b0060","doi-asserted-by":"crossref","first-page":"e614","DOI":"10.1371\/journal.pntd.0000614","article-title":"Bacillus thuringiensis Cry5B Protein Is Highly Efficacious as a Single-Dose Therapy against an Intestinal Roundworm Infection in Mice","volume":"4","author":"Hu","year":"2010","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"11","key":"10.1016\/j.ijpara.2023.05.001_b0065","doi-asserted-by":"crossref","first-page":"e1900","DOI":"10.1371\/journal.pntd.0001900","article-title":"Mechanistic and single-dose in vivo therapeutic studies of Cry5B anthelmintic action against hookworms","volume":"6","author":"Hu","year":"2012","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"18","key":"10.1016\/j.ijpara.2023.05.001_b0070","doi-asserted-by":"crossref","first-page":"5527","DOI":"10.1128\/AEM.01854-13","article-title":"Bacillus subtilis strain engineered for treatment of soil-transmitted helminth diseases","volume":"79","author":"Hu","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"issue":"5","key":"10.1016\/j.ijpara.2023.05.001_b0075","doi-asserted-by":"crossref","first-page":"e0006506","DOI":"10.1371\/journal.pntd.0006506","article-title":"In Vivo and in Vitro Studies of Cry5B and Nicotinic Acetylcholine Receptor Agonist Anthelmintics Reveal a Powerful and Unique Combination Therapy against Intestinal Nematode Parasites","volume":"12","author":"Hu","year":"2018","journal-title":"PLoS Negl. Trop. Dis."},{"issue":"2","key":"10.1016\/j.ijpara.2023.05.001_b0080","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.ijpddr.2018.05.001","article-title":"Bacillus thuringiensis Cry5B Protein as a New Pan-Hookworm Cure","volume":"8","author":"Hu","year":"2018","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"10.1016\/j.ijpara.2023.05.001_b0085","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ijpddr.2020.04.003","article-title":"Efficacy Evaluation of Anthelmintic Products against an Infection with the Canine Hookworm (Ancylostoma caninum) Isolate Worthy 4.1F3P in Dogs","volume":"13","author":"Jimenez Castro","year":"2020","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"10.1016\/j.ijpara.2023.05.001_b0090","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ijpddr.2021.08.005","article-title":"Multiple drug resistance in hookworms infecting greyhound dogs in the USA","volume":"17","author":"Jimenez Castro","year":"2021","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"issue":"10117","key":"10.1016\/j.ijpara.2023.05.001_b0095","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/S0140-6736(17)31930-X","article-title":"Soil-Transmitted helminth infections","volume":"391","author":"Jourdan","year":"2018","journal-title":"Lancet"},{"issue":"3","key":"10.1016\/j.ijpara.2023.05.001_b0110","doi-asserted-by":"crossref","first-page":"e01469","DOI":"10.1128\/AAC.01469-20","article-title":"Recombinant paraprobiotics as a new paradigm for treating gastrointestinal nematode parasites of humans","volume":"65","author":"Li","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpara.2023.05.001_b0115","doi-asserted-by":"crossref","first-page":"16088","DOI":"10.1038\/nrdp.2016.88","article-title":"Hookworm Infection","volume":"2","author":"Loukas","year":"2016","journal-title":"Nature Rev. Dis. Primers"},{"issue":"13\u201314","key":"10.1016\/j.ijpara.2023.05.001_b0120","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/j.ijpara.2021.11.001","article-title":"The Yin and Yang of Human Soil-Transmitted Helminth Infections","volume":"51","author":"Loukas","year":"2021","journal-title":"Int. J. Parasitol."},{"issue":"10","key":"10.1016\/j.ijpara.2023.05.001_b0125","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1084\/jem.20091903","article-title":"Regulation of pathogenesis and immunity in helminth infections","volume":"206","author":"Maizels","year":"2009","journal-title":"J. Exp. Med."},{"issue":"3","key":"10.1016\/j.ijpara.2023.05.001_b0130","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/nprot.2007.56","article-title":"A simple, robust and semi-automated parasite egg isolation protocol","volume":"2","author":"Mes","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ijpara.2023.05.001_b0135","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.639573","article-title":"Rodent models for the study of soil-transmitted helminths: a proteomics approach","volume":"11","author":"Monta\u00f1o","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"2","key":"10.1016\/j.ijpara.2023.05.001_b0140","doi-asserted-by":"crossref","first-page":"e12798","DOI":"10.1111\/pim.12798","article-title":"Immunopathology and modulation induced by hookworms: from understanding to intervention","volume":"43","author":"Mour\u00e3o Dias Magalh\u00e3es","year":"2021","journal-title":"Parasite Immunol."},{"issue":"4","key":"10.1016\/j.ijpara.2023.05.001_b0145","doi-asserted-by":"crossref","first-page":"648","DOI":"10.2307\/3283205","article-title":"Cryopreservation of human hookworms","volume":"80","author":"Nolan","year":"1994","journal-title":"J. Parasitol."},{"issue":"4","key":"10.1016\/j.ijpara.2023.05.001_b0150","doi-asserted-by":"crossref","first-page":"e0007345","DOI":"10.1371\/journal.pntd.0007345","article-title":"A highly expressed intestinal cysteine protease of Ancylostoma ceylanicum protects vaccinated hamsters from hookworm infection","volume":"13","author":"Noon","year":"2019","journal-title":"PLOS Negl. Trop. Dis."},{"issue":"12","key":"10.1016\/j.ijpara.2023.05.001_b0155","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1038\/ng1472","article-title":"A transcriptomic analysis of the phylum nematoda","volume":"36","author":"Parkinson","year":"2004","journal-title":"Nature Genet."},{"key":"10.1016\/j.ijpara.2023.05.001_b0160","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1756-3305-7-37","article-title":"Global numbers of infection and disease burden of soil transmitted helminth infections in 2010","volume":"7","author":"Pullan","year":"2014","journal-title":"Parasites Vectors"},{"issue":"6","key":"10.1016\/j.ijpara.2023.05.001_b0165","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1007\/s00281-012-0347-3","article-title":"Immunity to the model intestinal helminth parasite Heligmosomoides polygyrus","volume":"34","author":"Reynolds","year":"2012","journal-title":"Sem. Immunopathol."},{"issue":"5","key":"10.1016\/j.ijpara.2023.05.001_b0170","doi-asserted-by":"crossref","first-page":"e1008508","DOI":"10.1371\/journal.ppat.1008508","article-title":"Harnessing helminth-driven immunoregulation in the search for novel therapeutic modalities","volume":"16","author":"Ryan","year":"2020","journal-title":"PLoS Pathog."},{"issue":"1","key":"10.1016\/j.ijpara.2023.05.001_b0175","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s13071-018-2621-2","article-title":"Of Dogs and Hookworms: Man\u2019s best friend and his parasites as a model for translational biomedical research","volume":"11","author":"Shepherd","year":"2018","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.ijpara.2023.05.001_b0180","series-title":"The Nematode Caenorhabditis Elegans","article-title":"Methods","author":"Sulston","year":"1988"},{"issue":"1","key":"10.1016\/j.ijpara.2023.05.001_b0185","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s13052-021-00981-1","article-title":"Hookworm infection in infants: a case report and review of literature","volume":"47","author":"Umbrello","year":"2021","journal-title":"Ital. J. Pediat."},{"key":"10.1016\/j.ijpara.2023.05.001_b9585","doi-asserted-by":"crossref","unstructured":"Gill,\u00a0J.H.,\u00a0Redwin,\u00a0J.M.,\u00a0van Wyk,\u00a0J.A.,\u00a0Lacey,\u00a0E.,\u00a01995.\u00a0Avermectin inhibition of larval development in haemonchus contortus\u2013effects of ivermectin resistance.\u00a0Int. J. Parasitol.\u00a025\u00a0(4),\u00a0463\u2013470.","DOI":"10.1016\/0020-7519(94)00087-5"},{"key":"10.1016\/j.ijpara.2023.05.001_b329856","doi-asserted-by":"crossref","unstructured":"Kotze,\u00a0A.C.,\u00a0Stein,\u00a0P.A.,\u00a0Dobson,\u00a0R.J.,\u00a01999.\u00a0Investigation of intestinal nematode responses to naphthalophos and pyrantel using a larval development assay.\u00a0Int. J. Parasitol.\u00a029\u00a0(7),\u00a01093\u20131099.","DOI":"10.1016\/S0020-7519(99)00064-8"},{"key":"10.1016\/j.ijpara.2023.05.001_b259865","doi-asserted-by":"crossref","unstructured":"Kotze,\u00a0A.C.,\u00a0Dobson,\u00a0R.J.,\u00a0Tyrrell,\u00a0K.L.,\u00a0Stein,\u00a0P.A.,\u00a02002.\u00a0High-Level ivermectin resistance in a field isolate of haemonchus contortus associated with a low level of resistance in the larval stage: implications for resistance detection.\u00a0Vet. Parasitol.\u00a0108\u00a0(3),\u00a0255\u2013263.","DOI":"10.1016\/S0304-4017(02)00200-5"}],"container-title":["International Journal for Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751923000930?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0020751923000930?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:58:33Z","timestamp":1777417113000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0020751923000930"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["S0020751923000930"],"URL":"https:\/\/doi.org\/10.1016\/j.ijpara.2023.05.001","relation":{},"ISSN":["0020-7519"],"issn-type":[{"value":"0020-7519","type":"print"}],"subject":[],"published":{"date-parts":[[2023,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An efficient method for viable cryopreservation and recovery of hookworms and other gastrointestinal nematodes in the laboratory","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijpara.2023.05.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Author(s). Published by Elsevier Ltd on behalf of Australian Society for Parasitology.","name":"copyright","label":"Copyright"}]}}