{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T13:37:44Z","timestamp":1768743464265,"version":"3.49.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology: Drugs and Drug Resistance"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1016\/j.ijpddr.2020.10.008","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T07:57:00Z","timestamp":1604044620000},"page":"188-200","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Development and application of a high-throughput screening assay for identification of small molecule inhibitors of the P. falciparum reticulocyte binding-like homologue 5 protein"],"prefix":"10.1016","volume":"14","author":[{"given":"Brad E.","family":"Sleebs","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate E.","family":"Jarman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8673-3437","authenticated-orcid":false,"given":"Sonja","family":"Frolich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilson","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Healer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiwen","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle S.","family":"Lucet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny W.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan F.","family":"Cowman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijpddr.2020.10.008_bib1","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.cell.2019.05.025","article-title":"Human antibodies that slow erythrocyte invasion potentiate malaria-neutralizing antibodies","volume":"178","author":"Alanine","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib2","doi-asserted-by":"crossref","first-page":"10526","DOI":"10.1021\/acs.jmedchem.9b00761","article-title":"The development process for discovery and clinical advancement of modern antimalarials","volume":"62","author":"Ashton","year":"2019","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib3","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.ijpara.2008.10.006","article-title":"Reticulocyte-binding protein homologue 5 - an essential adhesin involved in invasion of human erythrocytes by Plasmodium falciparum","volume":"39","author":"Baum","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib4","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S1473-3099(13)70005-7","article-title":"Efficacy of RTS,S malaria vaccines: individual-participant pooled analysis of phase 2 data","volume":"13","author":"Bejon","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib5","doi-asserted-by":"crossref","first-page":"14378","DOI":"10.1073\/pnas.1009198107","article-title":"Isolation of viable Plasmodium falciparum merozoites to define erythrocyte invasion events and advance vaccine and drug development","volume":"107","author":"Boyle","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib6","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1093\/femsre\/fuz005","article-title":"Targeting malaria parasite invasion of red blood cells as an antimalarial strategy","volume":"43","author":"Burns","year":"2019","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib7","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.vaccine.2012.10.106","article-title":"A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitory antibodies that are effective across common PfRH5 genetic variants","volume":"31","author":"Bustamante","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib8","author":"Calculator Plugins were used for structure property prediction and calculation"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002199","article-title":"An EGF-like protein forms a complex with PfRh5 and is required for invasion of human erythrocytes by Plasmodium falciparum","volume":"7","author":"Chen","year":"2011","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib10","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.04187","article-title":"Crystal structure of PfRh5, an essential P. falciparum ligand for invasion of human erythrocytes","volume":"3","author":"Chen","year":"2014","journal-title":"Elife"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib11","doi-asserted-by":"crossref","first-page":"314","DOI":"10.3389\/fmicb.2014.00314","article-title":"Association of antibodies to Plasmodium falciparum reticulocyte binding protein homologue 5 with protection from clinical malaria","volume":"5","author":"Chiu","year":"2014","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib12","doi-asserted-by":"crossref","first-page":"3222","DOI":"10.1529\/biophysj.108.134973","article-title":"A quantitative model of thermal stabilization and destabilization of proteins by ligands","volume":"95","author":"Cimmperman","year":"2008","journal-title":"Biophys. J."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib13","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1021\/jm801605r","article-title":"Promiscuous aggregate-based inhibitors promote enzyme unfolding","volume":"52","author":"Coan","year":"2009","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib14","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.pt.2013.03.003","article-title":"Plasmodium rhoptry proteins: why order is important","volume":"29","author":"Counihan","year":"2013","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib15","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1083\/jcb.201206112","article-title":"The cellular and molecular basis for malaria parasite invasion of the human red blood cell","volume":"198","author":"Cowman","year":"2012","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib16","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/nature10606","article-title":"Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum","volume":"480","author":"Crosnier","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib17","doi-asserted-by":"crossref","first-page":"4259","DOI":"10.1021\/jm100254w","article-title":"Colloid formation by drugs in simulated intestinal fluid","volume":"53","author":"Doak","year":"2010","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib18","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.chom.2014.11.017","article-title":"A PfRH5-based vaccine is efficacious against Heterologous strain blood-stage Plasmodium falciparum infection in aotus monkeys","volume":"17","author":"Douglas","year":"2015","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib19","doi-asserted-by":"crossref","first-page":"245","DOI":"10.4049\/jimmunol.1302045","article-title":"Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5","volume":"192","author":"Douglas","year":"2014","journal-title":"J. Immunol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib20","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.pt.2015.02.001","article-title":"PfRH5 as a candidate vaccine for Plasmodium falciparum malaria","volume":"31","author":"Drew","year":"2015","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib21","unstructured":"Gagaring, K., Borboa, R., Francek, C., Chen, Z., Buenviaje, J., Plouffe, D., Winzeler, E., Brinker, A., Diagana, T., Taylor, J., Glynne, R., Chatterjee, A., Kuhen, K., Novartis-GNF Malaria Box. Genomics Institute of the Novartis Research Foundation (GNF), 10675 John Jay Hopkins Drive, San Diego CA 92121, USA and Novartis Institute for Tropical Disease, 10 Biopolis Road, Chromos # 05-01, 138 670 Singapore."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib22","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/nature09107","article-title":"Thousands of chemical starting points for antimalarial lead identification","volume":"465","author":"Gamo","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib23","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1080\/00397919708003052","article-title":"A direct and high yielding route to 2-(5-tetrazolyl) substituted benzopyran-4-ones: synthesis of pranlukast","volume":"27","author":"Geen","year":"2006","journal-title":"Synth. Commun."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib24","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nature09099","article-title":"Chemical genetics of Plasmodium falciparum","volume":"465","author":"Guiguemde","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib25","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.chom.2008.06.001","article-title":"Erythrocyte binding protein PfRH5 polymorphisms determine species-specific pathways of Plasmodium falciparum invasion","volume":"4","author":"Hayton","year":"2008","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib26","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.13030","article-title":"Neutralising antibodies block the function of Rh5\/Ripr\/CyRPA complex during invasion of Plasmodium falciparum into human erythrocytes","volume":"21","author":"Healer","year":"2019","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib27","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1038\/nchembio.1246","article-title":"Structure-guided design of a selective BCL-X(L) inhibitor","volume":"9","author":"Lessene","year":"2013","journal-title":"Nat. Chem. Biol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib28","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1365-2141.2012.09044.x","article-title":"Human erythrocyte remodelling during Plasmodium falciparum malaria parasite growth and egress","volume":"157","author":"Mbengue","year":"2012","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib29","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.immuni.2013.06.018","article-title":"The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism","volume":"39","author":"Murphy","year":"2013","journal-title":"Immunity"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib30","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1042\/BJ20131270","article-title":"Insights into the evolution of divergent nucleotide-binding mechanisms among pseudokinases revealed by crystal structures of human and mouse MLKL","volume":"457","author":"Murphy","year":"2014","journal-title":"Biochem. J."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib31","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1042\/BJ20131174","article-title":"A robust methodology to subclassify pseudokinases based on their nucleotide-binding properties","volume":"457","author":"Murphy","year":"2014","journal-title":"Biochem. J."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib32","doi-asserted-by":"crossref","first-page":"6902","DOI":"10.1021\/jm800669s","article-title":"Discovery of an orally bioavailable small molecule inhibitor of prosurvival B-cell lymphoma 2 proteins","volume":"51","author":"Park","year":"2008","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib33","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/j.str.2016.07.008","article-title":"Biochemical and structural insights into doublecortin-like kinase domain 1","volume":"24","author":"Patel","year":"2016","journal-title":"Structure"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib34","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.96381","article-title":"Human vaccination against RH5 induces neutralizing antimalarial antibodies that inhibit RH5 invasion complex interactions","volume":"2","author":"Payne","year":"2017","journal-title":"JCI Insight"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib35","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.ejmech.2014.05.019","article-title":"Design, synthesis and molecular docking studies of novel N-benzenesulfonyl-1,2,3,4-tetrahydroisoquinoline-based triazoles with potential anticancer activity","volume":"81","author":"Pingaew","year":"2014","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib36","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.pt.2020.04.003","article-title":"The RH5-CyRPA-Ripr complex as a malaria vaccine target","volume":"36","author":"Ragotte","year":"2020","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib37","doi-asserted-by":"crossref","first-page":"12323","DOI":"10.1016\/0040-4020(96)00719-3","article-title":"Malonic acid receptors with decarboxylative activity","volume":"52","author":"Raposo","year":"1996","journal-title":"Tetrahedron"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib38","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1073\/pnas.1415466112","article-title":"Multiprotein complex between the GPI-anchored CyRPA with PfRH5 and PfRipr is crucial for Plasmodium falciparum erythrocyte invasion","volume":"112","author":"Reddy","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib39","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1128\/IAI.00970-13","article-title":"Bacterially expressed full-length recombinant Plasmodium falciparum RH5 protein binds erythrocytes and elicits potent strain-transcending parasite-neutralizing antibodies","volume":"82","author":"Reddy","year":"2014","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib40","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1056\/NEJMoa1208394","article-title":"A phase 3 trial of RTS,S\/AS01 malaria vaccine in african infants","volume":"367","author":"RTS","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib41","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1038\/nm.3048","article-title":"ABT-199, a potent and selective BCL-2 inhibitor, achieves antitumor activity while sparing platelets","volume":"19","author":"Souers","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib42","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0062906","article-title":"The open access malaria box: a drug discovery catalyst for neglected diseases","volume":"8","author":"Spangenberg","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib43","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"Trager","year":"1976","journal-title":"Science"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib44","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1093\/infdis\/jit553","article-title":"Naturally acquired antibodies specific for Plasmodium falciparum reticulocyte-binding protein homologue 5 inhibit parasite growth and predict protection from malaria","volume":"209","author":"Tran","year":"2014","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib45","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.chom.2016.06.004","article-title":"Essential role of the PfRh5\/PfRipr\/CyRPA complex during Plasmodium falciparum invasion of erythrocytes","volume":"20","author":"Volz","year":"2016","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib46","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004670","article-title":"Revealing the sequence and resulting cellular morphology of receptor-ligand interactions during Plasmodium falciparum invasion of erythrocytes","volume":"11","author":"Weiss","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib47","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/1475-2875-9-152","article-title":"Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays","volume":"9","author":"Wilson","year":"2010","journal-title":"Malar. J."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib48","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s12915-015-0162-0","article-title":"Macrolides rapidly inhibit red blood cell invasion by the human malaria parasite, Plasmodium falciparum","volume":"13","author":"Wilson","year":"2015","journal-title":"BMC Biol."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib49","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1128\/AAC.01881-12","article-title":"Defining the timing of action of antimalarial drugs against Plasmodium falciparum","volume":"57","author":"Wilson","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib50","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/s41586-018-0779-6","article-title":"Structure of Plasmodium falciparum Rh5-CyRPA-Ripr invasion complex","volume":"565","author":"Wong","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.ijpddr.2020.10.008_bib51","doi-asserted-by":"crossref","first-page":"21214","DOI":"10.1074\/jbc.C110.129502","article-title":"Crystal structure of the entire ectodomain of gp130: insights into the molecular assembly of the tall cytokine receptor complexes","volume":"285","author":"Xu","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ijpddr.2020.10.008_bib52","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/978-1-4939-3673-1_5","article-title":"AlphaScreen-based assays: ultra-high-throughput screening for small-molecule inhibitors of challenging enzymes and protein-protein interactions","volume":"1439","author":"Yasgar","year":"2016","journal-title":"Methods Mol. Biol."}],"container-title":["International Journal for Parasitology: Drugs and Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211320720300397?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211320720300397?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:49:11Z","timestamp":1761922151000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211320720300397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":52,"alternative-id":["S2211320720300397"],"URL":"https:\/\/doi.org\/10.1016\/j.ijpddr.2020.10.008","relation":{},"ISSN":["2211-3207"],"issn-type":[{"value":"2211-3207","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and application of a high-throughput screening assay for identification of small molecule inhibitors of the P. falciparum reticulocyte binding-like homologue 5 protein","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology: Drugs and Drug Resistance","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijpddr.2020.10.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.","name":"copyright","label":"Copyright"}]}}