{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T08:29:05Z","timestamp":1793780945822,"version":"4.6.1"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002141","name":"Public Health England","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004167","name":"Medicines for Malaria Venture","doi-asserted-by":"publisher","award":["MMV RD\/15\/0017"],"award-info":[{"award-number":["MMV RD\/15\/0017"]}],"id":[{"id":"10.13039\/501100004167","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000278","name":"Department for International Development, UK Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000278","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/M021157\/1"],"award-info":[{"award-number":["MR\/M021157\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["International Journal for Parasitology: Drugs and Drug Resistance"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1016\/j.ijpddr.2021.07.002","type":"journal-article","created":{"date-parts":[[2021,7,24]],"date-time":"2021-07-24T03:30:47Z","timestamp":1627097447000},"page":"5-11","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"special_numbering":"C","title":["Ex vivo susceptibility to new antimalarial agents differs among human-infecting Plasmodium species"],"prefix":"10.1016","volume":"17","author":[{"given":"Donelly A.","family":"van Schalkwyk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Moon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u00eblle","family":"Duffey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Leroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin J.","family":"Sutherland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ijpddr.2021.07.002_bib1","doi-asserted-by":"crossref","first-page":"4361","DOI":"10.1128\/AAC.02948-15","article-title":"Adaptation of the [3H]hypoxanthine uptake assay for in vitro-cultured Plasmodium knowlesi malaria parasites","volume":"60","author":"Arnold","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib2","doi-asserted-by":"crossref","first-page":"e1038","DOI":"10.1016\/S2214-109X(17)30333-9","article-title":"Outbreak of human malaria caused by Plasmodium simium in the Atlantic Forest in Rio de Janeiro: a molecular epidemiological investigation","volume":"5","author":"Brasil","year":"2017","journal-title":"Lancet Glob Health"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib3","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1550-7408.1985.tb03016.x","article-title":"Observation on complete schizogony of Plasmodium vivax in vitro","volume":"32","author":"Brockelman","year":"1985","journal-title":"J. Protozool."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib4","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1186\/s12936-017-2034-2","article-title":"Measuring ex vivo drug susceptibility in Plasmodium vivax isolates from Cambodia","volume":"16","author":"Chaorattanakawee","year":"2017","journal-title":"Malar. J."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib5","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/S0035-9203(01)90113-0","article-title":"Ex-vivo short-term culture and developmental assessment of Plasmodium vivax","volume":"95","author":"Chotivanich","year":"2001","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib6","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1128\/AAC.16.6.710","article-title":"Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique","volume":"16","author":"Desjardins","year":"1979","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib7","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1186\/1475-2875-12-425","article-title":"Susceptibility of human Plasmodium knowlesi infections to anti-malarials","volume":"12","author":"Fatih","year":"2013","journal-title":"Malar. J."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib8","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/nature09107","article-title":"Thousands of chemical starting points for antimalarial lead identification","volume":"465","author":"Gamo","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib9","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1111\/cmi.12275","article-title":"Human red blood cell-adapted Plasmodium knowlesi parasites: a new model system for malaria research","volume":"16","author":"Gruring","year":"2014","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib10","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nature09099","article-title":"Chemical genetics of Plasmodium falciparum","volume":"465","author":"Guiguemde","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib11","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1128\/AAC.01103-10","article-title":"The presence of leukocytes in ex vivo assays significantly increases the 50-percent inhibitory concentrations of artesunate and chloroquine against Plasmodium vivax and Plasmodium falciparum","volume":"55","author":"Kaewpongsri","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib12","doi-asserted-by":"crossref","first-page":"5060","DOI":"10.1128\/AAC.02727-13","article-title":"KAF156 is an antimalarial clinical candidate with potential for use in prophylaxis, treatment, and prevention of disease transmission","volume":"58","author":"Kuhen","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib13","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1016\/j.ebiom.2015.07.033","article-title":"Natural infection of Plasmodium brasilianum in humans: man and monkey share quartan malaria parasites in the Venezuelan Amazon","volume":"2","author":"Lalremruata","year":"2015","journal-title":"EBioMedicine"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib14","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1038\/ncomms2612","article-title":"Expansion of host cellular niche can drive adaptation of a zoonotic malaria parasite to humans","volume":"4","author":"Lim","year":"2013","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib15","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1016\/S1473-3099(18)30309-8","article-title":"Antimalarial activity of single-dose DSM265, a novel plasmodium dihydroorotate dehydrogenase inhibitor, in patients with uncomplicated Plasmodium falciparum or Plasmodium vivax malaria infection: a proof-of-concept, open-label, phase 2a study","volume":"18","author":"Llanos-Cuentas","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib16","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1186\/s12936-017-1897-6","article-title":"Ex vivo susceptibility and genotyping of Plasmodium falciparum isolates from Pikine, Senegal","volume":"16","author":"Mbaye","year":"2017","journal-title":"Malar. J."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib17","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1073\/pnas.1216457110","article-title":"Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes","volume":"110","author":"Moon","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib18","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.ijpara.2011.01.004","article-title":"Plasmodium ovale curtisi and Plasmodium ovale wallikeri circulate simultaneously in African communities","volume":"41","author":"Oguike","year":"2011","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib19","doi-asserted-by":"crossref","first-page":"296ra111","DOI":"10.1126\/scitranslmed.aaa6645","article-title":"A long-duration dihydroorotate dehydrogenase inhibitor (DSM265) for prevention and treatment of malaria","volume":"7","author":"Phillips","year":"2015","journal-title":"Sci. Transl. Med."},{"issue":"12","key":"10.1016\/j.ijpddr.2021.07.002_bib20","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1021\/acsinfecdis.6b00144","article-title":"A triazolopyrimidine-based dihydroorotate dehydrogenase inhibitor with improved drug-like properties for treatment and prevention of malaria","volume":"2","author":"Phillips","year":"2016","journal-title":"ACS Infect. Dis."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib21","doi-asserted-by":"crossref","first-page":"9059","DOI":"10.1073\/pnas.0802982105","article-title":"In silico activity profiling reveals the mechanism of action of antimalarials discovered in a high-throughput screen","volume":"105","author":"Plouffe","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0016957","article-title":"The human serum metabolome","volume":"6","author":"Psychogios","year":"2011","journal-title":"PloS One"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib23","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.3201\/eid2608.200343","article-title":"Naturally acquired human Plasmodium cynomolgi and P. knowlesi infections, Malaysian borneo","volume":"26","author":"Raja","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib24","doi-asserted-by":"crossref","first-page":"e02519","DOI":"10.1128\/AAC.02519-17","article-title":"Enhanced ex vivo Plasmodium vivax intraerythrocytic enrichment and maturation for rapid and sensitive parasite growth assays","volume":"62","author":"Rangel","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib25","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1126\/science.1193225","article-title":"Spiroindolones, a potent compound class for the treatment of malaria","volume":"329","author":"Rottmann","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib26","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.ijpara.2012.08.010","article-title":"Human ex vivo studies on asexual Plasmodium vivax: the best way forward","volume":"42","author":"Russell","year":"2012","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib27","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1128\/AAC.47.1.170-173.2003","article-title":"Simple in vitro assay for determining the sensitivity of Plasmodium vivax isolates from fresh human blood to antimalarials in areas where P. vivax is endemic","volume":"47","author":"Russell","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib28","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/nature21038","article-title":"Plasmodium malariae and P. ovale genomes provide insights into malaria parasite evolution","volume":"542","author":"Rutledge","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib29","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/1475-2875-7-94","article-title":"Plasmodium vivax trophozoites insensitive to chloroquine","volume":"7","author":"Sharrock","year":"2008","journal-title":"Malar. J."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib30","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/S0140-6736(04)15836-4","article-title":"A large focus of naturally acquired Plasmodium knowlesi infections in human beings","volume":"363","author":"Singh","year":"2004","journal-title":"Lancet"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib31","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1093\/jac\/dkm104","article-title":"Chloroquine-resistant Plasmodium falciparum infections among UK travellers returning with malaria after chloroquine prophylaxis","volume":"59","author":"Sutherland","year":"2007","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib32","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1086\/652240","article-title":"Two nonrecombining sympatric forms of the human malaria parasite Plasmodium ovale occur globally","volume":"201","author":"Sutherland","year":"2010","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib33","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/1475-2875-13-68","article-title":"First case of a naturally acquired human infection with Plasmodium cynomolgi","volume":"13","author":"Ta","year":"2014","journal-title":"Malar. J."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib34","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"Trager","year":"1976","journal-title":"Science"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib35","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ijpddr.2019.02.004","article-title":"Plasmodium knowlesi exhibits distinct in vitro drug susceptibility profiles from those of Plasmodium falciparum","volume":"9","author":"van Schalkwyk","year":"2019","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"10.1016\/j.ijpddr.2021.07.002_bib36","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1186\/1475-2875-12-320","article-title":"Culture-adapted Plasmodium falciparum isolates from UK travellers: in vitro drug sensitivity, clonality and drug resistance markers","volume":"12","author":"van Schalkwyk","year":"2013","journal-title":"Malar. J."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib37","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.1093\/jac\/dkx279","article-title":"Comparison of the susceptibility of Plasmodium knowlesi and Plasmodium falciparum to antimalarial agents","volume":"72","author":"van Schalkwyk","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib38","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.02549-19","article-title":"Novel endochin-like quinolones exhibit potent in vitro activity against Plasmodium knowlesi but do not synergize with proguanil","volume":"64","author":"van Schalkwyk","year":"2020","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ijpddr.2021.07.002_bib39","series-title":"World Malaria Report 2020: 20 Years of Global Progress and Challenges","author":"World Health Organization","year":"2020"}],"container-title":["International Journal for Parasitology: Drugs and Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211320721000336?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2211320721000336?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T11:40:29Z","timestamp":1778845229000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2211320721000336"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":39,"alternative-id":["S2211320721000336"],"URL":"https:\/\/doi.org\/10.1016\/j.ijpddr.2021.07.002","relation":{},"ISSN":["2211-3207"],"issn-type":[{"value":"2211-3207","type":"print"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ex vivo susceptibility to new antimalarial agents differs among human-infecting Plasmodium species","name":"articletitle","label":"Article Title"},{"value":"International Journal for Parasitology: Drugs and Drug Resistance","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ijpddr.2021.07.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.","name":"copyright","label":"Copyright"}]}}